Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC16_RS11925 Genome accession   NZ_CP011775
Coordinates   2644269..2645954 (+) Length   561 a.a.
NCBI ID   WP_060530787.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2639269..2650954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC16_RS11905 (FORC16_2368) pdhR 2640884..2641651 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FORC16_RS11910 (FORC16_2370) ampD 2642040..2642612 (-) 573 WP_060530795.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC16_RS11915 (FORC16_2371) nadC 2642705..2643592 (+) 888 WP_060530792.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC16_RS11920 (FORC16_2372) - 2643812..2644264 (+) 453 WP_060530791.1 pilin -
  FORC16_RS11925 (FORC16_2373) pilB 2644269..2645954 (+) 1686 WP_060530787.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC16_RS11930 (FORC16_2374) pilC 2646003..2647229 (+) 1227 WP_045618629.1 type II secretion system F family protein Machinery gene
  FORC16_RS11935 (FORC16_2375) pilD 2647323..2648192 (+) 870 WP_013571138.1 prepilin peptidase Machinery gene
  FORC16_RS11940 (FORC16_2376) coaE 2648194..2648802 (+) 609 WP_060530785.1 dephospho-CoA kinase -
  FORC16_RS11945 (FORC16_2377) zapD 2648835..2649575 (+) 741 WP_011079546.1 cell division protein ZapD -
  FORC16_RS11950 (FORC16_2378) yacG 2649638..2649832 (+) 195 WP_060530783.1 DNA gyrase inhibitor YacG -
  FORC16_RS11955 (FORC16_2379) rplS 2650072..2650425 (-) 354 WP_060530781.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62280.26 Da        Isoelectric Point: 5.4002

>NTDB_id=148626 FORC16_RS11925 WP_060530787.1 2644269..2645954(+) (pilB) [Vibrio vulnificus strain FORC_016]
MTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPCTDLNQYDYQELCNQLGLREL
ITRHQALPLQKSNSGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELAN
LVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGMI
LMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADDL
IYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=148626 FORC16_RS11925 WP_060530787.1 2644269..2645954(+) (pilB) [Vibrio vulnificus strain FORC_016]
ATGACTAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAATACA
AGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAGTTAACCGAGCAGCTAAGCG
CGATTTTTGGCTTACCATGTACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTACGTGAGCTG
ATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACTCCGGTTTAATACTCGCGGTTGCAGACCCTACCAATATTCA
AGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGGCAA
TTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGTTGGCGAAT
TTGGTTGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCCCCCGTCAGTCGCTTTATCAA
CCAAATCCTGCTGGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGCGAT
TGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAGATC
CTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGCGAT
TGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGCAGCT
TGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATGATT
TTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGTGAA
TATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTTTTG
GTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAAACC
GCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAACCAT
TGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGGCTC
GTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGACTTG
ATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCATGCC
TTTTGATGAACAGCTTGCAGAGGCGATAGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAGGCA
TGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGTGTCCTCTAT
TTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

77.005

100

0.77
  pilB Vibrio parahaemolyticus RIMD 2210633

76.071

99.822

0.759
  pilB Vibrio cholerae strain A1552

74.555

100

0.747
  pilB Legionella pneumophila strain ERS1305867

52.827

91.444

0.483
  pilB Acinetobacter baumannii D1279779

51.533

93.048

0.48
  pilB Acinetobacter baylyi ADP1

50.478

93.226

0.471
  pilF Neisseria gonorrhoeae MS11

45.293

100

0.455
  pilF Thermus thermophilus HB27

40.787

99.643

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.474

0.364
  pilB Haemophilus influenzae Rd KW20

41.736

86.275

0.36

Multiple sequence alignment