Detailed information NTG0148628

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FORC16_RS11935
• Genome accession: NZ_CP011775
• Coordinates: 2647323..2648192 (+)
• Length: 289 a.a.
• NCBI ID: WP_013571138.1
• Uniprot ID: B7U4R6
• Organism: Vibrio vulnificus strain FORC_016
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2642323..2653192

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC16_RS11915 (FORC16_2371)	nadC	2642705..2643592 (+)	888	WP_060530792.1	carboxylating nicotinate-nucleotide diphosphorylase	-
FORC16_RS11920 (FORC16_2372)	-	2643812..2644264 (+)	453	WP_060530791.1	pilin	-
FORC16_RS11925 (FORC16_2373)	pilB	2644269..2645954 (+)	1686	WP_060530787.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FORC16_RS11930 (FORC16_2374)	pilC	2646003..2647229 (+)	1227	WP_045618629.1	type II secretion system F family protein	Machinery gene
FORC16_RS11935 (FORC16_2375)	pilD	2647323..2648192 (+)	870	WP_013571138.1	prepilin peptidase	Machinery gene
FORC16_RS11940 (FORC16_2376)	coaE	2648194..2648802 (+)	609	WP_060530785.1	dephospho-CoA kinase	-
FORC16_RS11945 (FORC16_2377)	zapD	2648835..2649575 (+)	741	WP_011079546.1	cell division protein ZapD	-
FORC16_RS11950 (FORC16_2378)	yacG	2649638..2649832 (+)	195	WP_060530783.1	DNA gyrase inhibitor YacG	-
FORC16_RS11955 (FORC16_2379)	rplS	2650072..2650425 (-)	354	WP_060530781.1	50S ribosomal protein L19	-
FORC16_RS11960 (FORC16_2380)	trmD	2650467..2651216 (-)	750	WP_011079543.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
FORC16_RS11965 (FORC16_2381)	rimM	2651244..2651792 (-)	549	WP_011079542.1	ribosome maturation factor RimM	-
FORC16_RS11970 (FORC16_2382)	rpsP	2651808..2652056 (-)	249	WP_011079541.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32249.29 Da
• Isoelectric Point: 6.4630

>NTDB_id=148628 FORC16_RS11935 WP_013571138.1 2647323..2648192(+) (pilD) [Vibrio vulnificus strain FORC_016]
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALLEISPVSLQDSVIGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLMGA

Nucleotide

• Length: 870 bp

>NTDB_id=148628 FORC16_RS11935 WP_013571138.1 2647323..2648192(+) (pilD) [Vibrio vulnificus strain FORC_016]
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTACCGAAAATTATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGCTCAAGTTGTCCTTCGTGCCACACCCCAATACGAGTTCGA
GATAATATTCCCGTCTTTAGTTGGTTAGCACTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGGTATCC
TTTTGTTGAAGCACTGAGCGCCTTTTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCGCTGA
TTGGTTTTACTTTGGTGTTGATCGCCGCCACCTTCATCGACTTAGATACCATGCTACTGCCTGATCAGCTCACGCTGCCG
CTGACGTGGGCAGGCATCGCGCTAGCGTTACTAGAGATAAGTCCAGTATCTCTGCAAGATTCAGTGATCGGCGCGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCCGATTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATATCTACCGATGATCATCTTACTCTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCAGATTGCAAAAGCAAGGCATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTGATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATGGGGGCATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: B7U4R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.163	100	0.772
pilD	Vibrio cholerae strain A1552	71.875	99.654	0.716
pilD	Acinetobacter nosocomialis M2	54.348	95.502	0.519
pilD	Acinetobacter baumannii D1279779	54.348	95.502	0.519
pilD	Neisseria gonorrhoeae MS11	48	95.156	0.457