Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VU14_RS02350 Genome accession   NZ_CP011100
Coordinates   526502..527743 (-) Length   413 a.a.
NCBI ID   WP_045788905.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 521502..532743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VU14_RS24090 (VU14_02320) - 522343..523526 (+) 1184 Protein_445 tetratricopeptide repeat protein -
  VU14_RS02325 (VU14_02325) mutT 523535..523948 (+) 414 WP_196299318.1 8-oxo-dGTP diphosphatase MutT -
  VU14_RS02330 (VU14_02330) yacG 524009..524203 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  VU14_RS02335 (VU14_02335) zapD 524213..524935 (-) 723 WP_024945431.1 cell division protein ZapD -
  VU14_RS02340 (VU14_02340) coaE 524973..525587 (-) 615 WP_045788903.1 dephospho-CoA kinase -
  VU14_RS02345 (VU14_02345) pilD 525607..526479 (-) 873 WP_045788904.1 prepilin peptidase Machinery gene
  VU14_RS02350 (VU14_02350) pilC 526502..527743 (-) 1242 WP_045788905.1 type II secretion system F family protein Machinery gene
  VU14_RS02355 (VU14_02355) pilB 527859..529565 (-) 1707 WP_045525681.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  VU14_RS22245 (VU14_02360) - 529569..530033 (-) 465 WP_045788906.1 pilin -
  VU14_RS02365 (VU14_02365) nadC 530356..531213 (-) 858 WP_045788907.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VU14_RS02370 (VU14_02370) - 531219..531689 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  VU14_RS02375 (VU14_02375) ampD 531837..532409 (+) 573 WP_045788908.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45436.56 Da        Isoelectric Point: 9.8734

>NTDB_id=142126 VU14_RS02350 WP_045788905.1 526502..527743(-) (pilC) [Aeromonas hydrophila strain AH10]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIA
LAIFLYVRAWRASQRVKDNTDKFVLTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=142126 VU14_RS02350 WP_045788905.1 526502..527743(-) (pilC) [Aeromonas hydrophila strain AH10]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGCGGGGTGAACCGCAAGGGCCA
GAAGGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAACAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATTGCCGTCGTCTCCCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTCCAAAGCCTGCAAATCATCGCTCGCAGCCACGAGAAGGCGGC
CATGCGCGAGCTCATGGGCCAGATTGCCGCCGATGTGGAGACCGGCACGCCCATGTCCGAGGCGCTGCGCCGCCATCCCC
GCCACTTTGACGATCTTTATTGCGATCTGGTGGAGGCTGGCGAGCAGTCCGGCGCTCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCGGAAGCACTCAAGTCCAAGATCAAGAAAGCCATGTTCTACCCCACCATGGTCATCCTGGT
GGCCATTGTCGTTACCTCCATCCTGCTGCTGTTTGTCATTCCCCAGTTCGAGGACATCTTCAAGAGTTTTGGCGCTGAGC
TGCCCATCTTCACCCAGTTCGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTATGTGATCTTTGGCGGCATAGCG
CTCGCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAGGGTCAAGGACAACACCGACAAGTTCGTACTGACCAT
CCCCGTGGTCGGCATGATATTGCACAAGGCCGCCATGGCCCGCTTCGCCCGCACCCTCTCCACGACCTTCTCTGCCGGTA
TCCCGCTGGTGGATGCCTTGGTTTCTGCAGCAGGCGCCTCTGGCAACTATGTCTATCGCACTGCCGTCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAAATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTTGCCACCATCTTCGAGCAGGAAGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTGGAGCCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGTGGCATGGTGGTCGCC
ATGTATCTGCCCATCTTCAAACTGGGGTCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.071

95.884

0.547
  pilC Acinetobacter baumannii D1279779

53.75

96.852

0.521
  pilC Acinetobacter baylyi ADP1

52.645

96.126

0.506
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Vibrio cholerae strain A1552

47.739

96.368

0.46
  pilC Vibrio campbellii strain DS40M4

46.65

97.579

0.455
  pilG Neisseria gonorrhoeae MS11

40

98.063

0.392
  pilG Neisseria meningitidis 44/76-A

39.753

98.063

0.39
  pilC Thermus thermophilus HB27

37.75

96.852

0.366

Multiple sequence alignment