Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VU14_RS02355 Genome accession   NZ_CP011100
Coordinates   527859..529565 (-) Length   568 a.a.
NCBI ID   WP_045525681.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AH10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522859..534565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VU14_RS02325 (VU14_02325) mutT 523535..523948 (+) 414 WP_196299318.1 8-oxo-dGTP diphosphatase MutT -
  VU14_RS02330 (VU14_02330) yacG 524009..524203 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  VU14_RS02335 (VU14_02335) zapD 524213..524935 (-) 723 WP_024945431.1 cell division protein ZapD -
  VU14_RS02340 (VU14_02340) coaE 524973..525587 (-) 615 WP_045788903.1 dephospho-CoA kinase -
  VU14_RS02345 (VU14_02345) pilD 525607..526479 (-) 873 WP_045788904.1 prepilin peptidase Machinery gene
  VU14_RS02350 (VU14_02350) pilC 526502..527743 (-) 1242 WP_045788905.1 type II secretion system F family protein Machinery gene
  VU14_RS02355 (VU14_02355) pilB 527859..529565 (-) 1707 WP_045525681.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  VU14_RS22245 (VU14_02360) - 529569..530033 (-) 465 WP_045788906.1 pilin -
  VU14_RS02365 (VU14_02365) nadC 530356..531213 (-) 858 WP_045788907.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VU14_RS02370 (VU14_02370) - 531219..531689 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  VU14_RS02375 (VU14_02375) ampD 531837..532409 (+) 573 WP_045788908.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VU14_RS02380 (VU14_02380) pdhR 532685..533449 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62497.87 Da        Isoelectric Point: 5.4342

>NTDB_id=142127 VU14_RS02355 WP_045525681.1 527859..529565(-) (pilB) [Aeromonas hydrophila strain AH10]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=142127 VU14_RS02355 WP_045525681.1 527859..529565(-) (pilB) [Aeromonas hydrophila strain AH10]
ATGACATCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGTTA
TCTGAGCCAAGCCAAGGCGCAGCGTAAACCCTTTGTGACCTTCCTGATCGAAAATGAAATTCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCATGTCCTGCCCATTTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGATGAATCCGAGATC
TCCGAATTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAAAGCGTCAATACGGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGCCAGAAGGCGCAATATCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCGCTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACTTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTAGGTGAAATCCGGGAT
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCCGAGGCTGAACTGCTTGAGCTGGGATTTACC
CAGCAGCAGCTGGCCGCAGGCTTGAGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment