Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VU14_RS02345 Genome accession   NZ_CP011100
Coordinates   525607..526479 (-) Length   290 a.a.
NCBI ID   WP_045788904.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520607..531479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VU14_RS24090 (VU14_02320) - 522343..523526 (+) 1184 Protein_445 tetratricopeptide repeat protein -
  VU14_RS02325 (VU14_02325) mutT 523535..523948 (+) 414 WP_196299318.1 8-oxo-dGTP diphosphatase MutT -
  VU14_RS02330 (VU14_02330) yacG 524009..524203 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  VU14_RS02335 (VU14_02335) zapD 524213..524935 (-) 723 WP_024945431.1 cell division protein ZapD -
  VU14_RS02340 (VU14_02340) coaE 524973..525587 (-) 615 WP_045788903.1 dephospho-CoA kinase -
  VU14_RS02345 (VU14_02345) pilD 525607..526479 (-) 873 WP_045788904.1 prepilin peptidase Machinery gene
  VU14_RS02350 (VU14_02350) pilC 526502..527743 (-) 1242 WP_045788905.1 type II secretion system F family protein Machinery gene
  VU14_RS02355 (VU14_02355) pilB 527859..529565 (-) 1707 WP_045525681.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  VU14_RS22245 (VU14_02360) - 529569..530033 (-) 465 WP_045788906.1 pilin -
  VU14_RS02365 (VU14_02365) nadC 530356..531213 (-) 858 WP_045788907.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32461.73 Da        Isoelectric Point: 6.5955

>NTDB_id=142125 VU14_RS02345 WP_045788904.1 525607..526479(-) (pilD) [Aeromonas hydrophila strain AH10]
MLLITDVFHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYLGYFNPETQPQQEERYNLMVPRSACPHCGHA
ITAMENIPLLSWLWLKGRCRECQAPISARYPLVELLTALLSLVVAATFPPGWGLLAALLLTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLAGGFAPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
VGAFIGIGLILLRNHHQNKPIPFGPYLAIAGWIALLWGDTITHWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=142125 VU14_RS02345 WP_045788904.1 525607..526479(-) (pilD) [Aeromonas hydrophila strain AH10]
ATGTTGCTGATCACTGACGTTTTTCACAGCCTGCCCTGGCTCTATTTCTCACTGGTATTCCTCTTTTCTCTGATGATCGG
CAGTTTTCTCAACGTGGTCATCCATCGTCTGCCCATCATGCTGGAGCGGGAGTGGCAGGCCGAATATCTCGGCTATTTCA
ATCCAGAGACACAGCCGCAGCAGGAAGAGCGCTACAACCTGATGGTGCCGCGTTCTGCCTGTCCTCATTGCGGCCACGCC
ATCACGGCCATGGAGAACATCCCACTGCTCAGCTGGCTCTGGCTCAAGGGGCGCTGCCGTGAATGCCAGGCCCCCATCTC
GGCCCGTTACCCCTTGGTGGAGCTGTTGACGGCCCTGCTCTCGCTGGTCGTCGCCGCAACATTCCCACCGGGTTGGGGCT
TACTGGCCGCCCTGTTGCTTACCTGGGTACTGGTGGCGCTCACTTTCATCGATCTGGACAAGATGCTGCTGCCGGATCAG
CTGACCCTGCCACTGCTGTGGGGTGGCCTGCTGTTCAACCTTGCAGGGGGATTTGCGCCGCTGGCCGATGCGGTGATCGG
TGCCATGGCCGGCTATCTGGTGCTCTGGAGCCTCTACTGGGCCTTCAAGTTGCTGACCGGCAAGGAAGGGATGGGCTATG
GCGACTTCAAGCTGTTGGCGGCGCTGGGTGCCTGGCTCGGCTGGCAGGCACTGCCCATCGTGTTGCTGCTCTCGTCGCTG
GTTGGCGCTTTCATCGGGATTGGTCTCATTCTGCTGCGCAACCATCATCAGAACAAACCCATCCCCTTCGGCCCCTATCT
GGCCATCGCCGGCTGGATCGCGCTGCTGTGGGGCGATACCATCACCCACTGGTACCTCACGACGTTTCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.672

98.966

0.61
  pilD Vibrio campbellii strain DS40M4

55.944

98.621

0.552
  pilD Acinetobacter baumannii D1279779

54.225

97.931

0.531
  pilD Acinetobacter nosocomialis M2

54.225

97.931

0.531
  pilD Neisseria gonorrhoeae MS11

48.352

94.138

0.455

Multiple sequence alignment