Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N805_RS23920 Genome accession   NZ_CP010979
Coordinates   5405810..5407078 (+) Length   422 a.a.
NCBI ID   WP_019472100.1    Uniprot ID   -
Organism   Pseudomonas putida S13.1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5400810..5412078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N805_RS23900 (N805_23905) - 5400815..5403265 (-) 2451 WP_371113227.1 penicillin-binding protein 1A -
  N805_RS23905 (N805_23910) pilM 5403459..5404340 (+) 882 WP_019472097.1 type IV pilus biogenesis protein PilM -
  N805_RS23910 (N805_23915) - 5404337..5404873 (+) 537 WP_019472098.1 PilN domain-containing protein -
  N805_RS23915 (N805_23920) - 5404870..5405886 (+) 1017 WP_026034528.1 pilus assembly protein PilP -
  N805_RS23920 (N805_23925) pilQ 5405810..5407078 (+) 1269 WP_019472100.1 type IV pilus secretin PilQ Machinery gene
  N805_RS23925 (N805_23930) aroK 5407083..5407601 (+) 519 WP_026034530.1 shikimate kinase AroK -
  N805_RS23930 (N805_23935) aroB 5407699..5408796 (+) 1098 WP_026034531.1 3-dehydroquinate synthase -
  N805_RS23935 (N805_23940) - 5408812..5410416 (+) 1605 WP_019472103.1 SPOR domain-containing protein -
  N805_RS30610 - 5410547..5410771 (+) 225 WP_132844974.1 hypothetical protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45829.56 Da        Isoelectric Point: 4.8368

>NTDB_id=141084 N805_RS23920 WP_019472100.1 5405810..5407078(+) (pilQ) [Pseudomonas putida S13.1.2]
MKNVMKWVLAALLPIPLASAAPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKGLARQQEGNVLLVAPAAAFAAQSFDAHLGLLPEVQLQPLRRELIPIHHANAADLVELLMTSLADDSTLAVRGSLGVDE
RTNTLVAHQPAERLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGENLRPGKELFIDLGVERAGSSV
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=141084 N805_RS23920 WP_019472100.1 5405810..5407078(+) (pilQ) [Pseudomonas putida S13.1.2]
ATGAAGAACGTGATGAAATGGGTGTTGGCAGCCCTGCTGCCGATCCCGCTGGCGTCGGCGGCCCCTTATCAGGGTGAGCC
CCTGTCATTGAACTTCCAGGATGTAGAAGTGCGCTCGGTGCTGCAGGTGCTGGCCGACTATGCGGGCGTCAACCTGGTTG
CCAGCGATGACGTGCAGGGCAACGTGACCTTGCGGTTGCAGGACGTGCCCTGGGACCAGGCCCTTGACCTGGTGCTGCGC
AGCAAGGGCCTTGCTCGGCAGCAGGAGGGCAATGTGCTGCTGGTGGCGCCTGCGGCAGCGTTTGCCGCGCAGTCGTTCGA
TGCTCATCTGGGCCTGTTGCCGGAGGTGCAGTTGCAGCCGTTACGCCGTGAATTGATACCGATCCACCATGCCAACGCTG
CCGATCTGGTTGAGCTGTTGATGACCAGTCTGGCGGATGACAGTACCCTCGCCGTTCGTGGCAGCCTGGGTGTCGATGAG
CGCACCAACACCTTGGTGGCGCACCAGCCCGCCGAGCGCCTGGCTGAATTGCGGCAACTGGTTGCGCAGCTGGATGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTACGAAAAAAGCCTGGGGGTGCGCTGGGGCG
GGCCGCTGTACGGTGAAAACCTGCGGCCGGGCAAGGAACTGTTCATCGACCTGGGCGTAGAGCGGGCGGGCAGCAGCGTC
GGCCTGGGCCTTCTGCGCGGCGACGTGCTGCTCGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCTTACCAGGAAACCA
GTAAGAGCGGCGCCACCTCGGTCGCCTTTCGCGAAGCCTCGCTGTCGCTGGAGGTGACCCCGCAAATTACCCCGGACAAC
AAGGTGATCATGGCGGTCAGGGTGACCAAGGATGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAACGCCAAGGTTCGTGTGGCGGACGGTGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTGGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(50-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.998

100

0.614
  comQ Acinetobacter baylyi ADP1

42.444

100

0.453
  pilQ Acinetobacter baumannii strain A118

41.266

100

0.448
  pilQ Acinetobacter baumannii D1279779

41.266

100

0.448
  pilQ Vibrio campbellii strain DS40M4

40.972

100

0.419
  pilQ Legionella pneumophila strain Lp02

42.344

99.052

0.419
  pilQ Legionella pneumophila strain ERS1305867

42.344

99.052

0.419
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.587

98.578

0.41
  pilQ Vibrio cholerae strain A1552

41.587

98.578

0.41
  pilQ Neisseria gonorrhoeae strain FA1090

35.681

100

0.36

Multiple sequence alignment