Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B723_RS00215 Genome accession   NZ_CP010945
Coordinates   47959..49176 (+) Length   405 a.a.
NCBI ID   WP_017340818.1    Uniprot ID   A0A0K1QGJ9
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42959..54176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS00195 (B723_00195) - 44424..44720 (+) 297 WP_017340814.1 DUF2845 domain-containing protein -
  B723_RS00200 (B723_00200) - 44957..45313 (-) 357 WP_017340815.1 BON domain-containing protein -
  B723_RS00205 (B723_00205) - 45620..46030 (-) 411 WP_017340816.1 pilin -
  B723_RS00210 (B723_00210) pilB 46256..47956 (+) 1701 WP_017340817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B723_RS00215 (B723_00215) pilC 47959..49176 (+) 1218 WP_017340818.1 type II secretion system F family protein Machinery gene
  B723_RS00220 (B723_00220) pilD 49178..50050 (+) 873 WP_017340819.1 A24 family peptidase Machinery gene
  B723_RS00225 (B723_00225) coaE 50047..50670 (+) 624 WP_017340820.1 dephospho-CoA kinase -
  B723_RS00230 (B723_00230) yacG 50667..50867 (+) 201 WP_017340821.1 DNA gyrase inhibitor YacG -
  B723_RS00235 (B723_00235) - 50877..51092 (-) 216 WP_017340822.1 hypothetical protein -
  B723_RS00240 (B723_00240) - 51156..51845 (-) 690 WP_017340823.1 energy-coupling factor ABC transporter permease -
  B723_RS00245 (B723_00245) - 52033..52275 (+) 243 WP_017340824.1 DUF2790 domain-containing protein -
  B723_RS00250 (B723_00250) - 52294..52941 (-) 648 WP_017340825.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44141.98 Da        Isoelectric Point: 10.0024

>NTDB_id=140787 B723_RS00215 WP_017340818.1 47959..49176(+) (pilC) [Pseudomonas fluorescens NCIMB 11764]
MAVKAAKISVYAWEGTDKKGTKMTGELSGQNPALIKAQLRKQGINPGKVRKKSAAIFSLGQRIKAHDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDDVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFKGLGAQLPAFTVMVIGLSQFMQDWWWVMLGALIAAIFGAR
HAFRTSQGFRDRTDTWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVIAMYLPIFQ
LGNAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=140787 B723_RS00215 WP_017340818.1 47959..49176(+) (pilC) [Pseudomonas fluorescens NCIMB 11764]
ATGGCGGTCAAAGCAGCGAAAATCAGCGTTTACGCCTGGGAAGGCACAGATAAAAAAGGCACGAAAATGACCGGCGAACT
CAGCGGTCAGAACCCGGCGCTGATCAAGGCGCAATTGCGCAAGCAAGGCATCAATCCCGGCAAGGTGCGCAAGAAGTCCG
CCGCGATTTTCAGCCTCGGCCAACGCATCAAGGCGCATGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGGGTGCCGCTGTTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGATAACCCGGCCATGCGCAAACTGGTGGACGA
CGTGAAACAGGAGGTCGCGGCGGGCAACAGTTTCGCCGCCGCCCTGCGTAAAAAACCGCAGTATTTCGATGAGCTCTACT
GCAACCTGGTGGATGCCGGCGAACAGGCCGGCGCGCTGGACACCCTGTTGGAGCGCGTCGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACGTATCCCCTGGCCGTGGTGTTCGTCGCGATCATCGTCACCGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCGAGTCGGTGTTCAAAGGATTAGGTGCGCAATTGCCGGCGTTCACGGTGATGG
TCATCGGTCTGTCGCAGTTCATGCAGGATTGGTGGTGGGTGATGCTGGGGGCTTTGATTGCTGCGATATTCGGTGCGCGC
CACGCCTTCAGGACATCACAAGGCTTTCGCGACCGGACCGACACCTGGCTGTTGAAACTGCCGCTGATTGGCACGCTGAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACACTGTCGACCACCTTTGCCGCTGGCGTGCCGCTGGTCGAGGCGCTGG
AATCGGTGGCGGGCGCGACCGGCAACATCGTGTTCAAGCGTGCGGTGCTGCGCATCAAGCAGGATGTGTCGACAGGGATG
CAGCTGAATTTCTCCATGCGCACCTCCGGCATCTTTCCGAACATGGCGATCCAGATGACCGCCATTGGCGAAGAGTCCGG
CGCACTGGACGACATGCTCGACAAGGTCGCGAGTTTCTATGAGGCCGAAGTCGACAACATGGTCGATAACCTCACCAGTC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTTATCGTCGGCGGCCTGGTGATTGCAATGTACCTGCCCATTTTCCAA
CTCGGCAATGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1QGJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

72.346

100

0.723
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilC Vibrio cholerae strain A1552

42.857

96.79

0.415
  pilG Neisseria meningitidis 44/76-A

41.542

99.259

0.412
  pilC Vibrio campbellii strain DS40M4

40.547

99.259

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment