Detailed information NTG0140788

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B723_RS00220
• Genome accession: NZ_CP010945
• Coordinates: 49178..50050 (+)
• Length: 290 a.a.
• NCBI ID: WP_017340819.1
• Uniprot ID: A0A0K1QHG7
• Organism: Pseudomonas fluorescens NCIMB 11764
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 44178..55050

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B723_RS00195 (B723_00195)	-	44424..44720 (+)	297	WP_017340814.1	DUF2845 domain-containing protein	-
B723_RS00200 (B723_00200)	-	44957..45313 (-)	357	WP_017340815.1	BON domain-containing protein	-
B723_RS00205 (B723_00205)	-	45620..46030 (-)	411	WP_017340816.1	pilin	-
B723_RS00210 (B723_00210)	pilB	46256..47956 (+)	1701	WP_017340817.1	type IV-A pilus assembly ATPase PilB	Machinery gene
B723_RS00215 (B723_00215)	pilC	47959..49176 (+)	1218	WP_017340818.1	type II secretion system F family protein	Machinery gene
B723_RS00220 (B723_00220)	pilD	49178..50050 (+)	873	WP_017340819.1	A24 family peptidase	Machinery gene
B723_RS00225 (B723_00225)	coaE	50047..50670 (+)	624	WP_017340820.1	dephospho-CoA kinase	-
B723_RS00230 (B723_00230)	yacG	50667..50867 (+)	201	WP_017340821.1	DNA gyrase inhibitor YacG	-
B723_RS00235 (B723_00235)	-	50877..51092 (-)	216	WP_017340822.1	hypothetical protein	-
B723_RS00240 (B723_00240)	-	51156..51845 (-)	690	WP_017340823.1	energy-coupling factor ABC transporter permease	-
B723_RS00245 (B723_00245)	-	52033..52275 (+)	243	WP_017340824.1	DUF2790 domain-containing protein	-
B723_RS00250 (B723_00250)	-	52294..52941 (-)	648	WP_017340825.1	methyl-accepting chemotaxis protein	-
B723_RS00255 (B723_00255)	-	52938..54206 (-)	1269	WP_017340826.1	OprD family porin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32003.40 Da
• Isoelectric Point: 8.0477

>NTDB_id=140788 B723_RS00220 WP_017340819.1 49178..50050(+) (pilD) [Pseudomonas fluorescens NCIMB 11764]
MPLNEILTLYPLAFVGIALVVGLLVGSFLNVVIWRLPKMLAREWRLQAYEILGLPGETPLPTYNLMLPHSQCPHCGHRIR
AWENIPLLSYVFLRGRCASCATPISKRYPLTELACGLLSAFIAWHFGFGGQAVMVLVLTWGLLAMSLIDAEHQLLPDVLV
LPLLWLGLIVNSFELFVPLTEALWGAIAGYMALWSVFWLFKLITGKDGIGHGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVIGVILLSLRNAKASTPIPFGPYLAIAGWIALLWGGQITDFYWQFVGLK

Nucleotide

• Length: 873 bp

>NTDB_id=140788 B723_RS00220 WP_017340819.1 49178..50050(+) (pilD) [Pseudomonas fluorescens NCIMB 11764]
ATGCCCCTGAACGAAATCCTGACCCTGTACCCGCTGGCGTTTGTCGGCATTGCACTGGTGGTCGGCTTGCTGGTCGGCAG
CTTCCTCAACGTAGTGATCTGGCGCCTGCCGAAAATGCTCGCGCGCGAATGGCGACTGCAGGCCTATGAAATTCTCGGCT
TGCCTGGCGAAACACCGCTCCCGACCTACAACCTGATGCTGCCCCATTCCCAATGCCCGCACTGTGGGCACCGGATCCGC
GCCTGGGAAAACATTCCGCTGCTCAGTTATGTGTTTTTACGTGGGCGATGCGCCAGCTGCGCAACCCCCATCAGCAAACG
CTATCCATTGACCGAACTGGCGTGCGGCCTGCTGTCAGCGTTCATCGCCTGGCATTTCGGTTTCGGTGGGCAGGCCGTGA
TGGTGCTGGTGTTGACCTGGGGTTTGTTGGCCATGAGCCTGATCGACGCCGAGCATCAGTTGTTGCCGGATGTACTGGTG
CTGCCACTGTTGTGGCTGGGATTGATCGTCAACAGCTTCGAGTTGTTCGTGCCATTGACCGAGGCACTGTGGGGCGCAAT
TGCTGGCTACATGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGATCACCGGCAAGGATGGCATCGGCCACGGCGATT
TCAAGCTCCTGGCCATGCTGGGCGCCTGGGGTGGCTGGCAGATTTTGCCGCTCACCATTCTGCTGTCGTCTCTGGTCGGT
GCCGTTATCGGCGTTATTTTGCTGAGCCTGCGCAACGCGAAAGCCTCGACGCCTATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGCTGGATTGCGTTGCTCTGGGGTGGTCAAATAACCGACTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A0K1QHG7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.128	97.241	0.507
pilD	Vibrio campbellii strain DS40M4	50.355	97.241	0.49
pilD	Acinetobacter baumannii D1279779	50	93.793	0.469
pilD	Acinetobacter nosocomialis M2	50	93.793	0.469
pilD	Neisseria gonorrhoeae MS11	50	93.103	0.466