Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B723_RS00210 Genome accession   NZ_CP010945
Coordinates   46256..47956 (+) Length   566 a.a.
NCBI ID   WP_017340817.1    Uniprot ID   -
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 41256..52956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS00190 (B723_00190) - 41473..44424 (+) 2952 WP_017340813.1 DUF748 domain-containing protein -
  B723_RS00195 (B723_00195) - 44424..44720 (+) 297 WP_017340814.1 DUF2845 domain-containing protein -
  B723_RS00200 (B723_00200) - 44957..45313 (-) 357 WP_017340815.1 BON domain-containing protein -
  B723_RS00205 (B723_00205) - 45620..46030 (-) 411 WP_017340816.1 pilin -
  B723_RS00210 (B723_00210) pilB 46256..47956 (+) 1701 WP_017340817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B723_RS00215 (B723_00215) pilC 47959..49176 (+) 1218 WP_017340818.1 type II secretion system F family protein Machinery gene
  B723_RS00220 (B723_00220) pilD 49178..50050 (+) 873 WP_017340819.1 A24 family peptidase Machinery gene
  B723_RS00225 (B723_00225) coaE 50047..50670 (+) 624 WP_017340820.1 dephospho-CoA kinase -
  B723_RS00230 (B723_00230) yacG 50667..50867 (+) 201 WP_017340821.1 DNA gyrase inhibitor YacG -
  B723_RS00235 (B723_00235) - 50877..51092 (-) 216 WP_017340822.1 hypothetical protein -
  B723_RS00240 (B723_00240) - 51156..51845 (-) 690 WP_017340823.1 energy-coupling factor ABC transporter permease -
  B723_RS00245 (B723_00245) - 52033..52275 (+) 243 WP_017340824.1 DUF2790 domain-containing protein -
  B723_RS00250 (B723_00250) - 52294..52941 (-) 648 WP_017340825.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62804.95 Da        Isoelectric Point: 5.8830

>NTDB_id=140786 B723_RS00210 WP_017340817.1 46256..47956(+) (pilB) [Pseudomonas fluorescens NCIMB 11764]
MNDIALSGLAKQLVMAELLTDKSAQQAYQQAQRQKLSLVSYLVQNKLVKSRQIAEIASEHFGMALLDLHCLDKESQPKGL
VSEKLLRQHNALPLWRRGNKLFVGVSDPTNYQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDSHNSGLEDMADVDLDG
VDIESIDDRNQDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLGFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKRPIDIPREALIKEGFPE
ERIGSFTIYEPVGCDQCNVGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=140786 B723_RS00210 WP_017340817.1 46256..47956(+) (pilB) [Pseudomonas fluorescens NCIMB 11764]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTCATGGCCGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACGCCAGAAACTCTCCCTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAAAGCCGTCAGATCG
CCGAGATCGCCTCCGAGCATTTCGGCATGGCGCTGCTGGACCTCCACTGCCTGGACAAAGAGTCCCAACCCAAAGGGCTG
GTCAGCGAAAAACTGCTTCGCCAGCACAACGCCCTGCCCCTCTGGCGGCGCGGCAACAAGTTGTTCGTCGGCGTTTCCGA
CCCGACCAATTACCAGGCTATCAACGATATCCAGTTCAGCACCGGGCTGAACACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAGAAATTTTTCGACAGCCACAATTCCGGCCTCGAAGACATGGCTGACGTCGACCTCGATGGC
GTCGACATCGAGTCGATCGACGACCGCAACCAGGACGCCATCGCCGGGCAAGACGCCGACGACGCGCCGGTGGTGCGCTT
TGTCCACAAGATGTTGCTCGACGCAATCAAGAGCGGCTCATCCGACCTGCACTTCGAACCCTACGAGAAAACCTACCGCG
TGCGGATGCGCACCGACGGCATGTTGCGTGAAGTCGCCAAGCCACCGATTCAACTGGCCAACCGCATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAATACCCTGCCGACCCTCTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CCCAAATGGGCATCGACGCCCTCGGGTACGAGCCGGACCAGAAAGACCTGTACATGGCCGCACTGAAGCAGCCACAAGGG
ATGATCCTGGTGACCGGGCCTACCGGTTCGGGCAAGACCGTGTCGCTCTATACCGGGCTGAATATCCTCAACACCGTGGA
CATCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAACCCCAAGCAGG
GACTGGGCTTTGCCCAGGCGCTGCGTTCGTTTTTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGTGACCTG
GAAACCGCGGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGGTTCAACATCGCCACCTCGGTCAGTCTGATCATCGCCCAGCGCC
TTGCGCGCAAACTGTGCAGCCATTGCAAGAGACCCATCGATATTCCCCGCGAGGCGTTGATCAAGGAAGGCTTCCCCGAG
GAACGCATTGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGACCAGTGCAACGTTGGCTACAAGGGGCGCGTGGGAAT
TTATGAAGTGGTGAAGAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGATATCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGTACTTCCGGGCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.511

99.647

0.583
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.96

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

50.528

100

0.507
  pilB Vibrio campbellii strain DS40M4

49.298

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.722

93.993

0.364

Multiple sequence alignment