Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RR42_RS17985 Genome accession   NZ_CP010536
Coordinates   3890808..3892073 (+) Length   421 a.a.
NCBI ID   WP_043349756.1    Uniprot ID   -
Organism   Cupriavidus basilensis strain 4G11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3885808..3897073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR42_RS17970 (RR42_m3658) - 3886687..3887076 (-) 390 WP_043349747.1 hypothetical protein -
  RR42_RS17975 (RR42_m3659) - 3887366..3888736 (+) 1371 WP_043349750.1 Fic family protein -
  RR42_RS17980 (RR42_m3660) pilF 3889023..3890777 (+) 1755 WP_043349753.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RR42_RS17985 (RR42_m3661) pilC 3890808..3892073 (+) 1266 WP_043349756.1 type II secretion system F family protein Machinery gene
  RR42_RS17990 (RR42_m3662) pilD 3892077..3892994 (+) 918 WP_236701932.1 prepilin peptidase Machinery gene
  RR42_RS17995 (RR42_m3663) coaE 3893055..3893666 (+) 612 WP_043349760.1 dephospho-CoA kinase -
  RR42_RS18000 (RR42_m3664) zapD 3893931..3894689 (+) 759 WP_043349763.1 cell division protein ZapD -
  RR42_RS18005 (RR42_m3665) - 3894694..3894879 (+) 186 WP_043349767.1 DNA gyrase inhibitor YacG -
  RR42_RS18010 (RR42_m3666) - 3894919..3895359 (-) 441 WP_043349769.1 NUDIX domain-containing protein -
  RR42_RS18015 (RR42_m3667) - 3895356..3896258 (-) 903 WP_043349772.1 ATP-binding protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46196.31 Da        Isoelectric Point: 10.2115

>NTDB_id=139156 RR42_RS17985 WP_043349756.1 3890808..3892073(+) (pilC) [Cupriavidus basilensis strain 4G11]
MATRAPAAAARKAAAPTGKPGRKAPTQYLFEWEGKDRKGKAFSGELRAESQAEVNATLRKQGLTVVKLKKRRAARGKKIT
QKDIAYFTRQLSTMLKAGIPLLQSIDIIARGHANPNFTQLLSEIRFDIESGSSMAQAFRRHPRYFDTLYCNLIDAGEQGG
ILDALLERLSLYMEKTIALKSQIKSAMIYPIAVLSVAFAVTVILMIFVIPAFKGVFSSFGANLPAPTLVVIAMSDFFVDN
WYLIIGAPIIGITMYIRGLKRSEKVQRASDRLLLKLPIFGSLFRKAVIARWTRTLATMFAAGTPLVESMESVAGAAGNWV
YYDATKEIEQSVRIGTSLTNAMQATHVFDSMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVAAISSLIEPLIIVVLG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=139156 RR42_RS17985 WP_043349756.1 3890808..3892073(+) (pilC) [Cupriavidus basilensis strain 4G11]
ATGGCGACACGCGCACCGGCCGCAGCCGCTCGCAAGGCGGCCGCGCCCACGGGGAAACCAGGGCGCAAGGCACCCACGCA
GTACCTGTTCGAGTGGGAAGGCAAGGACCGCAAGGGCAAGGCGTTCTCGGGCGAGCTGCGCGCGGAGAGTCAGGCCGAGG
TCAATGCCACGCTGCGAAAGCAGGGGCTGACGGTCGTCAAGCTGAAAAAGCGGCGGGCCGCGCGCGGGAAGAAGATTACC
CAGAAGGACATCGCGTACTTCACGCGCCAGCTGTCCACCATGCTCAAGGCGGGCATCCCGTTGCTGCAATCGATCGACAT
CATCGCGCGCGGGCATGCCAACCCCAACTTCACCCAGTTGCTGTCCGAGATCCGCTTTGACATCGAATCCGGCAGCAGCA
TGGCGCAGGCCTTCCGCCGCCATCCGCGCTACTTCGACACGCTTTACTGCAACCTGATCGATGCCGGCGAGCAGGGCGGT
ATCCTGGACGCCCTGCTGGAGCGCTTGTCGCTCTATATGGAAAAGACCATCGCGCTGAAGAGCCAGATCAAGTCAGCCAT
GATCTACCCGATCGCCGTGCTGAGCGTGGCCTTCGCGGTGACGGTCATCCTGATGATCTTCGTGATCCCGGCGTTCAAGG
GCGTGTTCTCCAGCTTCGGCGCCAACCTGCCGGCGCCGACCCTGGTAGTGATCGCCATGTCGGACTTCTTCGTCGACAAT
TGGTACCTGATCATCGGCGCGCCGATCATCGGCATCACGATGTACATACGCGGGCTAAAACGCTCGGAGAAGGTCCAGCG
CGCCAGTGACCGCCTGTTGCTCAAGCTGCCGATCTTCGGCTCGCTGTTCCGCAAAGCGGTGATCGCCCGCTGGACTCGCA
CGCTTGCCACCATGTTCGCCGCCGGCACGCCGCTGGTGGAATCGATGGAGTCGGTCGCCGGCGCCGCCGGCAACTGGGTC
TACTACGACGCCACCAAGGAAATCGAGCAATCGGTACGCATCGGTACCAGCCTGACCAACGCCATGCAGGCAACCCATGT
CTTCGATAGCATGGTGTTGCAGATGACGCAGATCGGCGAGGAATCCGGCGCGCTGGATAACATGCTGCTGAAGGTGGCGG
AGTTCTACGAGCGCGAGGTCGATGACGCTGTCGCCGCCATCTCCAGCCTGATCGAGCCGCTGATCATCGTGGTCCTGGGC
GTGCTGATTGGCGGCATGGTGGTGGCAATGTATCTTCCGATCTTCAAACTGGGACAGGTGGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

50.62

95.724

0.485
  pilG Neisseria gonorrhoeae MS11

50.127

93.349

0.468
  pilG Neisseria meningitidis 44/76-A

49.373

94.774

0.468
  pilC Acinetobacter baumannii D1279779

48.395

96.2

0.466
  pilC Legionella pneumophila strain ERS1305867

48.03

96.437

0.463
  pilC Acinetobacter baylyi ADP1

47.794

96.912

0.463
  pilC Vibrio campbellii strain DS40M4

39.461

96.912

0.382
  pilC Vibrio cholerae strain A1552

39.798

94.299

0.375
  pilC Thermus thermophilus HB27

37.5

98.812

0.371

Multiple sequence alignment