Detailed information NTG0139157

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RR42_RS17990
• Genome accession: NZ_CP010536
• Coordinates: 3892077..3892994 (+)
• Length: 305 a.a.
• NCBI ID: WP_236701932.1
• Uniprot ID: A0A0C4Y6H3
• Organism: Cupriavidus basilensis strain 4G11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3887077..3897994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RR42_RS17975 (RR42_m3659)	-	3887366..3888736 (+)	1371	WP_043349750.1	Fic family protein	-
RR42_RS17980 (RR42_m3660)	pilF	3889023..3890777 (+)	1755	WP_043349753.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RR42_RS17985 (RR42_m3661)	pilC	3890808..3892073 (+)	1266	WP_043349756.1	type II secretion system F family protein	Machinery gene
RR42_RS17990 (RR42_m3662)	pilD	3892077..3892994 (+)	918	WP_236701932.1	prepilin peptidase	Machinery gene
RR42_RS17995 (RR42_m3663)	coaE	3893055..3893666 (+)	612	WP_043349760.1	dephospho-CoA kinase	-
RR42_RS18000 (RR42_m3664)	zapD	3893931..3894689 (+)	759	WP_043349763.1	cell division protein ZapD	-
RR42_RS18005 (RR42_m3665)	-	3894694..3894879 (+)	186	WP_043349767.1	DNA gyrase inhibitor YacG	-
RR42_RS18010 (RR42_m3666)	-	3894919..3895359 (-)	441	WP_043349769.1	NUDIX domain-containing protein	-
RR42_RS18015 (RR42_m3667)	-	3895356..3896258 (-)	903	WP_043349772.1	ATP-binding protein	-
RR42_RS18020 (RR42_m3668)	argJ	3896360..3897589 (-)	1230	WP_043349776.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 305 a.a.
• Molecular weight: 32323.19 Da
• Isoelectric Point: 6.3690

>NTDB_id=139157 RR42_RS17990 WP_236701932.1 3892077..3892994(+) (pilD) [Cupriavidus basilensis strain 4G11]
MWLFSADGASGAISSLAAPAQTIAELAALPPWFLVAAAALIGLLVGSFLNVVVHRLPRMMEHDEANYIASLRDDPIPHPE
PYNLMVPRSACPHCGHAIGALENIPVLSYLFLRGRCSSCAAPIGARYPLVEAGTALLTALATAHFGPTWQALAAIALIWA
LIALTLIDADTQLLPDQITLPLVWLGLLLNLGGLFAPLADAVIGAAAGYLLLWTVYWIFKLVRGKEGMGYGDFKLMGALG
AWFGWQALPALVLLSSVVGLVFALANIALRRQDRDTHFAFGPYIAGAGLLVLFFGPNVLPLGIVG

Nucleotide

• Length: 918 bp

>NTDB_id=139157 RR42_RS17990 WP_236701932.1 3892077..3892994(+) (pilD) [Cupriavidus basilensis strain 4G11]
ATGTGGCTGTTTTCCGCGGATGGCGCCAGTGGCGCGATTTCTTCACTGGCTGCCCCCGCGCAGACCATTGCCGAGCTGGC
GGCGCTGCCGCCCTGGTTCCTGGTCGCCGCCGCCGCGCTGATCGGCTTGCTGGTCGGCAGCTTCCTCAACGTGGTGGTGC
ACCGCCTGCCGCGCATGATGGAGCATGACGAAGCCAACTATATCGCGTCGCTGCGCGACGACCCGATCCCCCACCCCGAG
CCCTACAACCTGATGGTGCCGCGCTCGGCGTGCCCACACTGCGGACACGCCATTGGCGCCCTGGAGAACATTCCGGTACT
GAGCTACCTGTTCCTGCGCGGGCGCTGCTCATCGTGCGCCGCGCCCATCGGCGCGCGCTATCCCCTGGTGGAGGCGGGCA
CCGCGCTGCTCACCGCGCTGGCCACCGCGCACTTCGGCCCGACCTGGCAGGCTCTCGCTGCCATCGCCCTGATCTGGGCC
CTGATCGCCCTCACCCTGATCGACGCCGACACGCAGCTACTGCCCGACCAGATCACGCTGCCGCTGGTATGGCTGGGCCT
GCTGCTCAATCTCGGCGGGCTGTTCGCACCGCTGGCGGATGCCGTGATCGGCGCGGCCGCCGGCTACCTGCTGCTGTGGA
CGGTGTACTGGATCTTCAAGCTGGTGCGGGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGCTGATGGGCGCGCTGGGC
GCCTGGTTCGGCTGGCAGGCCCTGCCGGCGCTGGTGCTGCTCTCCTCCGTGGTGGGGCTGGTTTTCGCGCTTGCCAATAT
CGCGCTGCGCCGTCAGGATCGCGATACGCACTTCGCCTTCGGCCCTTATATCGCCGGAGCCGGGCTGCTTGTGCTGTTCT
TTGGGCCCAACGTGCTGCCGCTGGGCATTGTGGGCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (40-145)

• Type IV leader peptidase family (Peptidase_A24) (156-264)

3D structure

• AlphaFold DB: A0A0C4Y6H3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.763	91.475	0.492
pilD	Vibrio campbellii strain DS40M4	51.439	91.148	0.469
pilD	Neisseria gonorrhoeae MS11	48.529	89.18	0.433
pilD	Acinetobacter nosocomialis M2	46.715	89.836	0.42
pilD	Acinetobacter baumannii D1279779	47.037	88.525	0.416