Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   RR42_RS17980 Genome accession   NZ_CP010536
Coordinates   3889023..3890777 (+) Length   584 a.a.
NCBI ID   WP_043349753.1    Uniprot ID   -
Organism   Cupriavidus basilensis strain 4G11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3884023..3895777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR42_RS17950 (RR42_m3653) rpmA 3884147..3884407 (-) 261 WP_006162509.1 50S ribosomal protein L27 -
  RR42_RS17955 (RR42_m3654) rplU 3884477..3884788 (-) 312 WP_006162510.1 50S ribosomal protein L21 -
  RR42_RS17960 (RR42_m3656) ispB 3885361..3886290 (+) 930 WP_052494691.1 octaprenyl diphosphate synthase -
  RR42_RS17970 (RR42_m3658) - 3886687..3887076 (-) 390 WP_043349747.1 hypothetical protein -
  RR42_RS17975 (RR42_m3659) - 3887366..3888736 (+) 1371 WP_043349750.1 Fic family protein -
  RR42_RS17980 (RR42_m3660) pilF 3889023..3890777 (+) 1755 WP_043349753.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RR42_RS17985 (RR42_m3661) pilC 3890808..3892073 (+) 1266 WP_043349756.1 type II secretion system F family protein Machinery gene
  RR42_RS17990 (RR42_m3662) pilD 3892077..3892994 (+) 918 WP_236701932.1 prepilin peptidase Machinery gene
  RR42_RS17995 (RR42_m3663) coaE 3893055..3893666 (+) 612 WP_043349760.1 dephospho-CoA kinase -
  RR42_RS18000 (RR42_m3664) zapD 3893931..3894689 (+) 759 WP_043349763.1 cell division protein ZapD -
  RR42_RS18005 (RR42_m3665) - 3894694..3894879 (+) 186 WP_043349767.1 DNA gyrase inhibitor YacG -
  RR42_RS18010 (RR42_m3666) - 3894919..3895359 (-) 441 WP_043349769.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 64228.78 Da        Isoelectric Point: 7.0953

>NTDB_id=139155 RR42_RS17980 WP_043349753.1 3889023..3890777(+) (pilF) [Cupriavidus basilensis strain 4G11]
MTLGLALAQSRRIAPALLAQLEQSAREKKSQLIDEIIASGTMTAHDVAIFAADKYQLPLIDLNQYNLNKVPPALAGNREF
HAHRLLPLGRRENRLVLALSDPGNQAGLDAIKQKYNVPVEPVVVEHDKLMQYVRSASDDSSGSIKSIGPGHVERKMIDYD
PVASAGALRNKTAADIDDAPVVRFLHKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPVDIRDKISTRIKVL
SRLDISEKRVPQDGRMKLLIALPKEKGGDKDGKDSKEPKETVEKAIDFRVSTLPTLFGEKIVIRILESSSDKLDIDQLGY
EPAQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNEKAGLTFSAALRS
FLRQDPDIIMVGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTCKR
PGELAREVLADAGFQDEDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEPMQQIILAHGTALEIAEQARKDGVLSL
REAGLAKVKQGLTSLEEVLATTNM

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=139155 RR42_RS17980 WP_043349753.1 3889023..3890777(+) (pilF) [Cupriavidus basilensis strain 4G11]
ATGACACTTGGTCTAGCTCTTGCACAGAGCCGGCGTATCGCGCCGGCCCTGCTCGCCCAGCTTGAACAGTCGGCACGAGA
AAAAAAGTCGCAGCTGATCGACGAGATCATTGCCAGCGGCACCATGACTGCACATGATGTGGCGATCTTCGCGGCTGATA
AGTATCAGCTGCCGCTGATCGATCTGAATCAGTACAACCTCAACAAGGTGCCGCCGGCGCTGGCGGGCAATCGCGAGTTC
CACGCGCACCGCCTGCTGCCACTGGGCCGGCGCGAGAACCGCCTGGTGCTGGCGTTGTCCGACCCGGGCAACCAGGCCGG
GCTGGACGCCATCAAGCAGAAATACAACGTGCCGGTGGAACCCGTCGTGGTCGAGCACGACAAGCTGATGCAATATGTGC
GCTCGGCTTCCGACGACAGCAGCGGCTCGATCAAGTCGATCGGTCCCGGCCATGTCGAGCGCAAGATGATCGACTACGAC
CCGGTGGCGTCCGCCGGGGCGCTGCGCAACAAGACCGCGGCCGACATCGACGACGCGCCAGTGGTTCGCTTCCTGCACAA
GCTGCTGACGGAAGCCTTCCATCGCGGCGCCTCCGATCTTCACTTCGAGCCGTTCGAGACCTTCTACCGCATCCGCTTCC
GGGTGGATGGCGTGTTGCAGGAAGTGGCACGGCCGCCAGTGGACATCCGCGACAAGATCTCCACCCGGATCAAGGTGCTG
TCGCGCCTGGACATCTCGGAAAAGCGCGTGCCGCAAGATGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGAAAAGGG
CGGCGACAAAGACGGCAAGGACAGCAAGGAGCCCAAGGAAACCGTGGAGAAGGCGATCGACTTCCGCGTCTCCACGCTGC
CGACGCTGTTCGGCGAGAAGATCGTGATCCGGATCCTGGAGTCATCGTCCGACAAGCTCGACATCGACCAGCTCGGCTAC
GAGCCGGCGCAAAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAG
CGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCGGCTGAACCAGGGCGACATCAATATCTCGACCGCCGAGGATCCGG
CGGAAATCCAGTTGCCCGGCATCAACCAGGTCAACGTCAACGAAAAAGCCGGCCTGACTTTCTCCGCCGCGCTGCGCTCC
TTCCTGCGCCAGGATCCCGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGACATCTCGATCAAGGCTGC
ACAGACTGGCCACCTGGTGTTCTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCCTGATGAACATGGGTG
TCGCGCCGTTCAATATCGCGTCGAGCGTGCTGATGATCACCGCGCAGCGGCTTGCGCGGCGGCTGTGCACCTGCAAGCGC
CCCGGCGAGCTGGCGCGCGAGGTGCTGGCCGACGCCGGCTTCCAGGACGAGGACCTCGACGGCAGCTGGCAGCCTTACCA
CCCGGTCGGGTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGCATCTACCAGGTGATGCCGATCACCGAGC
CGATGCAGCAGATCATCCTCGCGCATGGCACGGCGCTGGAAATCGCCGAACAAGCGCGTAAAGATGGCGTGCTATCGTTG
CGCGAAGCTGGCCTGGCCAAGGTGAAGCAGGGTCTCACCTCGCTCGAAGAAGTGCTGGCCACAACAAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-260)

Type II/IV secretion system protein (T2SSE)

(281-474)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.169

100

0.509
  pilB Acinetobacter baumannii D1279779

48.801

100

0.488
  pilB Acinetobacter baylyi ADP1

48.858

97.432

0.476
  pilB Legionella pneumophila strain ERS1305867

47.007

97.26

0.457
  pilB Vibrio cholerae strain A1552

43.739

99.829

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

45.554

94.349

0.43
  pilB Vibrio campbellii strain DS40M4

44.485

94.692

0.421
  pilF Thermus thermophilus HB27

37.329

100

0.373

Multiple sequence alignment