Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Achr_RS15345 Genome accession   NZ_CP010415
Coordinates   3419825..3421042 (+) Length   405 a.a.
NCBI ID   WP_039805682.1    Uniprot ID   A0A0C4WPB5
Organism   Azotobacter chroococcum NCIMB 8003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3414825..3426042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS15320 (Achr_30430) - 3415004..3415249 (+) 246 WP_039805677.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  Achr_RS15325 (Achr_30440) - 3415246..3415632 (+) 387 WP_039805678.1 type II toxin-antitoxin system VapC family toxin -
  Achr_RS15330 (Achr_30450) - 3415738..3416280 (-) 543 WP_039805679.1 REP-associated tyrosine transposase -
  Achr_RS24295 (Achr_30460) - 3416541..3417053 (-) 513 WP_039805680.1 pilin -
  Achr_RS25245 - 3417231..3417473 (+) 243 WP_144411563.1 hypothetical protein -
  Achr_RS15340 (Achr_30470) pilB 3417599..3419299 (+) 1701 WP_039805681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Achr_RS15345 (Achr_30480) pilC 3419825..3421042 (+) 1218 WP_039805682.1 type II secretion system F family protein Machinery gene
  Achr_RS15350 (Achr_30490) pilD 3421045..3421914 (+) 870 WP_039805683.1 prepilin peptidase Machinery gene
  Achr_RS15355 (Achr_30500) coaE 3422063..3422671 (+) 609 WP_039805684.1 dephospho-CoA kinase -
  Achr_RS15360 (Achr_30510) - 3422702..3423847 (-) 1146 WP_039805685.1 RNA-guided endonuclease InsQ/TnpB family protein -
  Achr_RS15365 (Achr_30520) yacG 3423977..3424177 (+) 201 WP_039805686.1 DNA gyrase inhibitor YacG -
  Achr_RS15370 (Achr_30530) - 3424294..3424980 (-) 687 WP_039805687.1 energy-coupling factor ABC transporter permease -
  Achr_RS15375 (Achr_30540) - 3424977..3425444 (-) 468 WP_039805688.1 hypothetical protein -
  Achr_RS15380 (Achr_30550) - 3425441..3425866 (-) 426 WP_039805689.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44266.81 Da        Isoelectric Point: 9.8270

>NTDB_id=136991 Achr_RS15345 WP_039805682.1 3419825..3421042(+) (pilC) [Azotobacter chroococcum NCIMB 8003]
MAEKALKTSFFTWEGTDRRGAKVKGELSGQNPALVKAQLRKQGINPTRVRKKAASLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQAFDIISEGFDNPNMRTLVDEVKQGVAAGNSFASALRKKPQYFDDLYCNLVDSGEQSGALENLLDRVATYKEKT
EALKAKIKKAMTYPIAVVVVAIVVSAILLIKVVPQFESVFANFGAELPAFTQMVINLSETLQKSWYYVLFGMFVVAFTLK
QAHQRSEKFRNWTDRTLLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVEALDSVSGATGNVVFRTATEKVKADVTTGM
QLNFSMRTTGTFPTMAIQMTAIGEESGALDEMLDKVASFYEAEVDNMVDSLTSLMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=136991 Achr_RS15345 WP_039805682.1 3419825..3421042(+) (pilC) [Azotobacter chroococcum NCIMB 8003]
ATGGCGGAAAAAGCGTTAAAGACCAGTTTCTTCACCTGGGAGGGCACCGACCGGCGCGGTGCCAAGGTCAAGGGCGAACT
GAGCGGGCAGAATCCGGCGCTGGTCAAGGCGCAGCTGCGCAAGCAGGGCATCAACCCGACCAGGGTGCGCAAGAAGGCCG
CCTCGCTGTTCGGCGCCGGCAAGAAGATCAAGCCGATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCTGGCGTGCCGCTGCTGCAGGCCTTCGACATCATCAGCGAGGGCTTCGACAACCCGAACATGCGCACGCTGGTGGATGA
AGTGAAGCAGGGGGTGGCGGCCGGCAACAGCTTCGCCAGCGCCCTGCGCAAGAAGCCGCAGTACTTCGACGACCTCTACT
GCAACCTGGTGGACTCCGGCGAGCAGTCCGGCGCCCTGGAAAACCTGCTGGACCGGGTCGCCACCTACAAGGAGAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCGATGACCTACCCCATCGCGGTGGTGGTGGTGGCGATCGTCGTCTCGGCGAT
CCTGCTGATCAAGGTGGTGCCGCAGTTCGAATCGGTGTTCGCCAACTTCGGTGCCGAGCTGCCGGCCTTTACCCAGATGG
TCATCAACCTGTCGGAAACGCTGCAGAAATCCTGGTACTACGTGCTGTTCGGCATGTTCGTCGTGGCCTTCACCCTCAAG
CAGGCGCACCAGCGCTCGGAGAAGTTCCGCAACTGGACGGACCGCACCCTGCTCAAGCTGCCGATCGTCGGCGACATTCT
CTACAAATCGGCGGTGGCCCGCTTCGCCCGCACCCTGTCCACCACCTTCGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCGGTGTCCGGCGCCACCGGCAACGTGGTGTTCCGCACCGCCACCGAGAAGGTCAAGGCCGATGTCACCACCGGCATG
CAGCTGAACTTTTCCATGCGCACCACCGGCACCTTCCCGACCATGGCGATCCAGATGACCGCGATTGGCGAGGAATCCGG
CGCGCTGGACGAGATGCTCGACAAGGTGGCGAGCTTCTATGAAGCCGAGGTGGACAACATGGTGGACAGCCTGACCAGCC
TGATGGAGCCATTGATCATGTCGGTGCTTGGCGTGCTGGTCGGCGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTGGGCGCGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4WPB5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.716

100

0.827
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Acinetobacter baumannii D1279779

59.259

100

0.593
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

44.527

99.259

0.442
  pilG Neisseria meningitidis 44/76-A

44.527

99.259

0.442
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

42.677

97.778

0.417
  pilC Thermus thermophilus HB27

37.811

99.259

0.375

Multiple sequence alignment