Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Achr_RS15340 Genome accession   NZ_CP010415
Coordinates   3417599..3419299 (+) Length   566 a.a.
NCBI ID   WP_039805681.1    Uniprot ID   A0A0C4WS72
Organism   Azotobacter chroococcum NCIMB 8003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3412599..3424299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS15300 (Achr_30390) - 3413223..3413714 (-) 492 WP_052263967.1 GNAT family N-acetyltransferase -
  Achr_RS15305 (Achr_30400) - 3413711..3413971 (-) 261 WP_039805674.1 DUF1778 domain-containing protein -
  Achr_RS15310 (Achr_30410) vapC 3414098..3414505 (-) 408 WP_039805675.1 type II toxin-antitoxin system tRNA(fMet)-specific endonuclease VapC -
  Achr_RS15315 (Achr_30420) - 3414505..3414735 (-) 231 WP_039805676.1 antitoxin -
  Achr_RS15320 (Achr_30430) - 3415004..3415249 (+) 246 WP_039805677.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  Achr_RS15325 (Achr_30440) - 3415246..3415632 (+) 387 WP_039805678.1 type II toxin-antitoxin system VapC family toxin -
  Achr_RS15330 (Achr_30450) - 3415738..3416280 (-) 543 WP_039805679.1 REP-associated tyrosine transposase -
  Achr_RS24295 (Achr_30460) - 3416541..3417053 (-) 513 WP_039805680.1 pilin -
  Achr_RS25245 - 3417231..3417473 (+) 243 WP_144411563.1 hypothetical protein -
  Achr_RS15340 (Achr_30470) pilB 3417599..3419299 (+) 1701 WP_039805681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Achr_RS15345 (Achr_30480) pilC 3419825..3421042 (+) 1218 WP_039805682.1 type II secretion system F family protein Machinery gene
  Achr_RS15350 (Achr_30490) pilD 3421045..3421914 (+) 870 WP_039805683.1 prepilin peptidase Machinery gene
  Achr_RS15355 (Achr_30500) coaE 3422063..3422671 (+) 609 WP_039805684.1 dephospho-CoA kinase -
  Achr_RS15360 (Achr_30510) - 3422702..3423847 (-) 1146 WP_039805685.1 RNA-guided endonuclease InsQ/TnpB family protein -
  Achr_RS15365 (Achr_30520) yacG 3423977..3424177 (+) 201 WP_039805686.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62262.20 Da        Isoelectric Point: 5.4836

>NTDB_id=136990 Achr_RS15340 WP_039805681.1 3417599..3419299(+) (pilB) [Azotobacter chroococcum NCIMB 8003]
MSNDISLPGLARQMVLAGLIDEKTAQQAQQQAQRNQTPLVTWLVQNKLVKSRALAELAAEQFGIALFDLKTLDRESQPRD
LLSEKLIRQHRALPLWRRGNRLFVAISDPTNHEAIREIRFGTGLNTEAILVEDDRLGEAIEKYFEGTGTGLDDLADADLD
GLDTEAASQKDDEADPSGGAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKSYRVRFRTDGILHEAAKPPVQLAPKIAAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDLRVNTLPTLWGEKTVMRILDPSSAQMGIDALGYEAEQKALYLAALSQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCACKKAVDIPREALLEEGFPE
ERIGTFKLYAPVGCDNCNGGYKGRVGIYEVVKNTPALQRIIMEDGNSIEIARQMRADGFNDLRTSALLKAMQGVTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=136990 Achr_RS15340 WP_039805681.1 3417599..3419299(+) (pilB) [Azotobacter chroococcum NCIMB 8003]
ATGAGCAACGACATTTCCCTCCCCGGCCTGGCACGGCAGATGGTGCTGGCCGGGCTGATCGACGAGAAGACCGCTCAGCA
GGCCCAACAGCAGGCACAACGCAACCAGACCCCGCTGGTCACCTGGCTGGTGCAGAACAAGCTGGTCAAGAGCAGGGCGC
TGGCGGAGCTGGCTGCCGAGCAGTTCGGCATCGCCCTGTTCGACCTCAAGACGCTGGACAGGGAGAGCCAGCCCCGGGAT
CTGCTCAGCGAGAAGCTGATCCGCCAGCACCGTGCCCTGCCGCTGTGGCGGCGCGGCAACCGGCTGTTCGTGGCGATTTC
CGACCCTACCAATCACGAGGCGATCCGCGAAATCCGCTTCGGCACCGGGCTGAACACCGAGGCCATCCTCGTCGAGGACG
ACCGGCTGGGCGAGGCCATCGAGAAATACTTCGAGGGCACCGGCACCGGCCTGGACGACCTGGCCGATGCCGACCTGGAT
GGCCTGGACACCGAGGCGGCCTCCCAGAAGGACGACGAGGCCGACCCGAGCGGCGGCGCCGAGGATGCGCCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCGATCCGCGGCGGTTCCTCGGACCTGCACTTCGAACCCTACGAGAAGAGCTACC
GGGTGCGCTTTCGTACCGACGGCATCCTCCACGAGGCGGCCAAACCACCGGTGCAGCTGGCGCCGAAGATCGCCGCGCGG
CTGAAGGTGATGGCCGGGCTGGACATTTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAACTGTCGAAGAC
CAAGGCCATCGACTTACGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAGACGGTGATGCGGATTCTCGACCCGTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGCGGAACAGAAGGCGCTGTACCTGGCGGCGCTGAGCCAGCCGCAG
GGCATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTATCCCTGTACACCGGCCTGAACATCCTCAACACCCC
GGAGGTGAACATCTCCACCGCGGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCACAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCGCAACATGGGCGTGCCCTCCTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTGTGCGCCTGCAAGAAGGCGGTGGACATTCCCCGCGAGGCGCTGCTCGAGGAAGGCTTTCCGGAG
GAGCGCATCGGCACCTTCAAGCTCTACGCCCCGGTCGGCTGCGACAACTGCAACGGCGGCTACAAGGGCCGGGTCGGCAT
TTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGACGGCAACTCCATCGAAATCGCCCGGCAGA
TGCGCGCCGACGGCTTCAACGACCTGCGCACATCGGCCCTGCTGAAAGCCATGCAGGGCGTCACCAGCCTGGAAGAAGTC
AACCGCGTCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4WS72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.708

99.823

0.606
  pilB Acinetobacter baylyi ADP1

58.494

100

0.59
  pilB Legionella pneumophila strain ERS1305867

54.336

99.823

0.542
  pilB Vibrio cholerae strain A1552

50.719

98.233

0.498
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.638

97.527

0.484
  pilB Vibrio campbellii strain DS40M4

48.49

99.47

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.52

100

0.406
  pilF Thermus thermophilus HB27

38.121

99.647

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.377

93.64

0.378

Multiple sequence alignment