Detailed information NTG0136992

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Achr_RS15350
• Genome accession: NZ_CP010415
• Coordinates: 3421045..3421914 (+)
• Length: 289 a.a.
• NCBI ID: WP_039805683.1
• Uniprot ID: A0A0C4WV24
• Organism: Azotobacter chroococcum NCIMB 8003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	3422702..3423769	3421045..3421914	flank	788

Gene organization within MGE regions

Location: 3421045..3423769

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Achr_RS15350 (Achr_30490)	pilD	3421045..3421914 (+)	870	WP_039805683.1	prepilin peptidase	Machinery gene
Achr_RS15355 (Achr_30500)	coaE	3422063..3422671 (+)	609	WP_039805684.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31610.56 Da
• Isoelectric Point: 6.8942

>NTDB_id=136992 Achr_RS15350 WP_039805683.1 3421045..3421914(+) (pilD) [Azotobacter chroococcum NCIMB 8003]
MSIHDLLAGPGPAFAFWAGLLGLVVGSFLNVVIHRLPKMMLRDWQAQAREVLELPGEVPGEPFNLLLPHSHCPQCGHEIK
PWENIPLLGWLALRGRCSACKARIGARYPLVELACGLLSAHVAWHYGFGWQAGAMLLLGWGLLAMSLIDYDHQLLPDSLV
LPLLWLGLILNNFGLFVSLEDALWGAVAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGILILRLRKAESGTPIPFGPFLALAGWIALLWGEQITGSYLQFAGL

Nucleotide

• Length: 870 bp

>NTDB_id=136992 Achr_RS15350 WP_039805683.1 3421045..3421914(+) (pilD) [Azotobacter chroococcum NCIMB 8003]
ATGTCCATTCATGATCTTCTCGCCGGACCCGGGCCGGCCTTCGCTTTCTGGGCGGGACTGCTGGGCCTGGTGGTCGGCAG
CTTTCTCAACGTGGTGATCCATCGCCTGCCGAAGATGATGCTGCGCGACTGGCAGGCGCAGGCGCGGGAAGTGCTGGAAC
TGCCTGGCGAAGTGCCGGGTGAGCCCTTCAACCTGCTGCTGCCCCACTCGCATTGCCCGCAGTGCGGGCACGAGATCAAG
CCCTGGGAGAACATCCCGCTGCTCGGCTGGCTGGCCCTGCGCGGCCGCTGCTCGGCCTGCAAGGCGCGGATCGGTGCGCG
CTATCCGCTGGTGGAGCTGGCCTGCGGGCTGCTCTCGGCTCATGTCGCCTGGCATTACGGCTTCGGCTGGCAGGCCGGCG
CCATGCTGCTGCTCGGCTGGGGCCTCCTGGCCATGAGCCTGATCGACTACGATCATCAGCTGCTGCCGGACTCGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCCTCAACAACTTCGGGCTGTTCGTTTCCCTGGAGGACGCCCTCTGGGGCGCGGT
GGCCGGCTATCTCAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCTACGGCGATT
TCAAGCTGCTGGCGATGCTCGGCGCCTGGGGCGGCTGGCAGATCCTGCCACTGACCATCCTGCTCTCCTCGCTGGTCGGC
GCCGTGCTGGGAATCCTCATCCTGCGCCTGCGCAAGGCCGAAAGCGGCACGCCGATCCCCTTCGGCCCCTTCCTCGCCCT
CGCCGGCTGGATTGCCCTGCTCTGGGGCGAGCAGATCACCGGCAGCTATCTGCAGTTCGCCGGTTTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A0C4WV24

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.569	94.81	0.536
pilD	Vibrio campbellii strain DS40M4	53.48	94.464	0.505
pilD	Acinetobacter nosocomialis M2	49.814	93.08	0.464
pilD	Acinetobacter baumannii D1279779	49.814	93.08	0.464
pilD	Neisseria gonorrhoeae MS11	49.81	91.003	0.453