Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SanJ4211_RS01235 Genome accession   NZ_CP012805
Coordinates   225616..226557 (+) Length   313 a.a.
NCBI ID   WP_003027001.1    Uniprot ID   -
Organism   Streptococcus anginosus strain J4211     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220616..231557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SanJ4211_RS01210 (SanJ4211_0213) - 220927..222126 (+) 1200 WP_057489559.1 phosphoglycerate kinase -
  SanJ4211_RS01215 (SanJ4211_0214) - 222641..223171 (+) 531 WP_003032280.1 FUSC family protein -
  SanJ4211_RS01220 (SanJ4211_0215) - 223248..223607 (+) 360 WP_003028467.1 MerR family transcriptional regulator -
  SanJ4211_RS01225 (SanJ4211_0216) glnA 223646..224992 (+) 1347 WP_003027006.1 type I glutamate--ammonia ligase -
  SanJ4211_RS01230 (SanJ4211_0217) - 225181..225546 (+) 366 WP_003027003.1 DUF1033 family protein -
  SanJ4211_RS01235 (SanJ4211_0218) comGA 225616..226557 (+) 942 WP_003027001.1 competence type IV pilus ATPase ComGA Machinery gene
  SanJ4211_RS01240 (SanJ4211_0219) comGB 226499..227521 (+) 1023 WP_041783985.1 competence type IV pilus assembly protein ComGB Machinery gene
  SanJ4211_RS01245 (SanJ4211_0220) comGC 227518..227835 (+) 318 WP_003028473.1 competence type IV pilus major pilin ComGC Machinery gene
  SanJ4211_RS01250 (SanJ4211_0221) comGD 227795..228223 (+) 429 WP_021001232.1 competence type IV pilus minor pilin ComGD Machinery gene
  SanJ4211_RS01255 (SanJ4211_0222) comGE/cglE 228195..228488 (+) 294 WP_021001233.1 competence type IV pilus minor pilin ComGE Machinery gene
  SanJ4211_RS01260 (SanJ4211_0223) comGF/cglF 228472..228909 (+) 438 WP_041783987.1 competence type IV pilus minor pilin ComGF Machinery gene
  SanJ4211_RS01265 comGG/cglG 228890..229216 (+) 327 WP_021001234.1 competence type IV pilus minor pilin ComGG Machinery gene
  SanJ4211_RS01270 (SanJ4211_0225) comGH 229288..230241 (+) 954 WP_021001235.1 class I SAM-dependent methyltransferase Machinery gene
  SanJ4211_RS01275 (SanJ4211_0226) - 230288..231487 (+) 1200 WP_057489560.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35907.12 Da        Isoelectric Point: 6.3128

>NTDB_id=136473 SanJ4211_RS01235 WP_003027001.1 225616..226557(+) (comGA) [Streptococcus anginosus strain J4211]
MVQNIAQAIICQAKKEQAQDIYFVPRENMYELYMRIGDERRFIQQYAFEEMAAVISHFKFTAGMNVGEKRRSQLGSCDYQ
YGDKKTSLRLSTVGDYRGYESLVIRLLHDEEAELKFWFAQIPELKERFKQRGLYLFSGPVGSGKTTLMHQLAQERFADQQ
IMSIEDPVEIKQAGMLQLQLNEAIGLTYESLIKLSLRHRPDLLIIGEIRDAETARAVVRASLTGVTVFSTIHAKSVRGVY
ERLLELGVTEAELKIVLQGICYQRLIAKGGIVDFVSERYQEHEPSDWNKQIDQLYAAGHVTFEQAETEKIIHS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=136473 SanJ4211_RS01235 WP_003027001.1 225616..226557(+) (comGA) [Streptococcus anginosus strain J4211]
ATGGTTCAAAACATTGCACAGGCTATTATTTGCCAAGCTAAAAAAGAGCAGGCTCAGGACATTTATTTTGTTCCAAGGGA
AAATATGTATGAGCTATACATGCGAATTGGAGATGAACGGCGCTTTATCCAACAGTATGCATTTGAAGAGATGGCAGCAG
TAATTAGTCATTTTAAGTTTACCGCTGGGATGAATGTCGGCGAAAAACGTAGAAGCCAGTTAGGATCTTGTGATTATCAA
TATGGTGATAAGAAAACGTCATTACGACTGTCAACAGTAGGGGATTATCGTGGATACGAAAGTCTGGTGATTCGTTTATT
GCATGATGAAGAGGCAGAGTTGAAGTTTTGGTTTGCTCAAATCCCAGAGTTAAAAGAACGCTTCAAACAACGAGGGCTTT
ATCTTTTTTCGGGACCGGTAGGAAGTGGTAAAACTACTCTCATGCACCAGTTGGCTCAAGAGCGTTTTGCCGATCAGCAA
ATCATGTCAATTGAAGATCCGGTTGAAATCAAACAAGCAGGGATGTTACAGCTGCAGCTGAACGAAGCGATTGGTTTAAC
TTATGAAAGTTTGATTAAACTTTCTCTTCGGCATCGACCAGATTTATTGATTATCGGAGAGATTCGTGATGCGGAGACAG
CAAGGGCAGTTGTTCGAGCTAGCTTAACGGGAGTGACTGTTTTTTCAACTATTCATGCTAAAAGCGTACGAGGTGTCTAT
GAACGGTTGCTGGAGTTGGGAGTGACAGAAGCAGAGCTGAAAATTGTTTTGCAAGGAATTTGCTACCAACGGTTAATTGC
GAAAGGAGGAATAGTGGATTTTGTCAGTGAAAGGTATCAAGAGCATGAGCCAAGTGACTGGAATAAGCAGATTGATCAGC
TTTATGCAGCAGGACATGTCACTTTTGAGCAGGCAGAAACGGAAAAAATTATCCATAGTTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Streptococcus gordonii str. Challis substr. CH1

82.258

99.042

0.815
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

75.719

100

0.757
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae D39

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae R6

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

75.399

100

0.754
  comGA Streptococcus mutans UA159

64.309

99.361

0.639
  comGA Streptococcus mutans UA140

64.309

99.361

0.639
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

54.341

99.361

0.54
  comGA Latilactobacillus sakei subsp. sakei 23K

42.349

89.776

0.38