Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   RP72_RS19100 Genome accession   NZ_CP010314
Coordinates   3621662..3623053 (-) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3616662..3628053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP72_RS19065 (RP72_19065) flgL 3616833..3617729 (-) 897 WP_003228004.1 flagellar hook-associated protein FlgL -
  RP72_RS19070 (RP72_19070) flgK 3617740..3619263 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  RP72_RS19075 (RP72_19075) flgN 3619282..3619764 (-) 483 WP_003227999.1 flagellar protein FlgN -
  RP72_RS19080 (RP72_19080) flgM 3619780..3620046 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  RP72_RS19085 (RP72_19085) yvyF 3620127..3620546 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  RP72_RS19090 (RP72_19090) comFC 3620620..3621342 (-) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  RP72_RS19095 (RP72_19095) comFB 3621306..3621602 (-) 297 WP_003227989.1 late competence protein ComFB -
  RP72_RS19100 (RP72_19100) comFA 3621662..3623053 (-) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  RP72_RS19105 (RP72_19105) fakBA 3623159..3624004 (-) 846 WP_003244125.1 DegV family protein -
  RP72_RS19110 (RP72_19110) degU 3624102..3624791 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  RP72_RS19115 (RP72_19115) degS 3624874..3626031 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  RP72_RS19120 (RP72_19120) yvyE 3626248..3626901 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=136306 RP72_RS19100 WP_003243962.1 3621662..3623053(-) (comFA) [Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=136306 RP72_RS19100 WP_003243962.1 3621662..3623053(-) (comFA) [Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment