Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OM33_RS13895 Genome accession   NZ_CP009888
Coordinates   3009244..3010479 (+) Length   411 a.a.
NCBI ID   WP_038642565.1    Uniprot ID   A0A0A7EHI9
Organism   Pseudoalteromonas piratica strain OCN003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3004244..3015479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM33_RS13875 (OM33_13875) ampD 3004973..3005518 (-) 546 WP_052141016.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OM33_RS13880 (OM33_13880) nadC 3005714..3006568 (+) 855 WP_038642561.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OM33_RS23055 - 3006957..3007082 (+) 126 Protein_2681 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OM33_RS13890 (OM33_13890) pilB 3007463..3009151 (+) 1689 WP_038642563.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM33_RS13895 (OM33_13895) pilC 3009244..3010479 (+) 1236 WP_038642565.1 type II secretion system F family protein Machinery gene
  OM33_RS13900 (OM33_13900) pilD 3010486..3011370 (+) 885 WP_038642567.1 A24 family peptidase Machinery gene
  OM33_RS13905 (OM33_13905) coaE 3011372..3011962 (+) 591 WP_038642569.1 dephospho-CoA kinase -
  OM33_RS13910 (OM33_13910) pilB 3012094..3013776 (+) 1683 WP_038642571.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM33_RS13915 (OM33_13915) yacG 3013860..3014093 (+) 234 WP_038642573.1 DNA gyrase inhibitor YacG -
  OM33_RS13920 (OM33_13920) - 3014153..3014602 (-) 450 WP_038642575.1 Spy/CpxP family protein refolding chaperone -
  OM33_RS13925 (OM33_13925) - 3014758..3015441 (+) 684 WP_038642577.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44396.06 Da        Isoelectric Point: 9.4569

>NTDB_id=132725 OM33_RS13895 WP_038642565.1 3009244..3010479(+) (pilC) [Pseudoalteromonas piratica strain OCN003]
MAKNENNKGLTTFSWVGVNARGKRMEGELNGPNIAIVKAQLRKQGITPSKVKKKAQPLFGMNGDKPIKPKDMAVFTRQLA
TMLLAGVSLIQALDMIANGSENKSLQKLITKIADEVKAGDPLSKALRANPKYFDELYCDLVESGEQSGALDRIFDRVALY
KEKAEALKSKIKKAMFYPVAVLTIAFIVTVILLIFVVPQFEQVFAGFGAELPAFTQFVIGISEFMQAYWWIVFGIIGAGF
MLFKRAHQSSKQVRDNTDKAILKLPVVGLILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRDAILDIKNEV
SSGNQMNFAMKNSGIFPDMVVQMVAIGEESGSLDEMLGKVASIYESEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGKIVGG

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=132725 OM33_RS13895 WP_038642565.1 3009244..3010479(+) (pilC) [Pseudoalteromonas piratica strain OCN003]
ATGGCAAAAAATGAAAATAACAAAGGCCTAACCACATTTTCCTGGGTCGGCGTTAACGCTCGGGGTAAACGTATGGAAGG
TGAGCTTAACGGTCCAAACATTGCGATTGTTAAGGCTCAGTTACGAAAACAAGGTATTACACCTTCCAAAGTTAAAAAGA
AAGCACAACCTTTATTTGGAATGAATGGTGATAAGCCTATCAAACCCAAAGATATGGCTGTTTTCACTCGCCAATTAGCC
ACAATGCTATTGGCAGGTGTATCACTTATTCAAGCGCTGGATATGATAGCCAATGGTTCAGAGAATAAATCATTACAAAA
GCTCATAACTAAAATTGCTGATGAAGTTAAAGCGGGTGATCCCTTAAGCAAAGCCCTCAGAGCAAATCCAAAATATTTTG
ATGAACTATACTGTGATTTGGTAGAATCCGGTGAACAGTCTGGTGCTTTGGACAGAATTTTTGACCGAGTAGCGCTTTAT
AAAGAAAAGGCTGAAGCACTGAAGTCAAAGATTAAAAAAGCCATGTTTTACCCTGTTGCTGTTTTAACCATTGCGTTTAT
AGTAACTGTCATTCTCCTTATCTTTGTTGTACCACAATTTGAACAAGTATTTGCCGGTTTTGGGGCAGAGCTTCCAGCCT
TCACTCAGTTTGTTATTGGCATTTCGGAATTTATGCAAGCCTATTGGTGGATTGTATTTGGTATTATTGGTGCAGGCTTC
ATGCTGTTTAAGCGCGCCCACCAATCAAGCAAACAAGTCCGAGACAACACTGATAAAGCTATCCTCAAATTGCCTGTTGT
AGGACTAATACTTAATAAAGCTGCTGTTGCCCGTTACGCACGCACTCTTTCAACAACCTTTGCCGCTGGTGTCCCCTTGG
TAGACGCTTTAGATTCAGCTGCTGGCGCTTCAGGTAATGCAGTTTATCGCGATGCCATTTTAGATATTAAAAATGAAGTA
AGTTCAGGTAATCAAATGAACTTTGCTATGAAGAATTCTGGCATCTTTCCTGATATGGTTGTTCAAATGGTAGCTATTGG
TGAAGAGTCTGGTTCACTTGACGAAATGCTGGGTAAAGTTGCAAGTATCTACGAATCTGAAGTCGATGATGCTGTAGATG
GCTTATCAAGTCTTTTAGAACCGCTTATTATGGCTGTGTTAGGTGTATTAGTTGGTGGTTTAATTGTTGCTATGTATCTT
CCTATCTTCCAGTTAGGTAAGATTGTTGGTGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7EHI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.25

97.324

0.596
  pilC Legionella pneumophila strain ERS1305867

55.172

98.783

0.545
  pilC Acinetobacter baylyi ADP1

54.75

97.324

0.533
  pilC Acinetobacter baumannii D1279779

53.25

97.324

0.518
  pilC Vibrio cholerae strain A1552

44.389

97.567

0.433
  pilC Vibrio campbellii strain DS40M4

42.469

98.54

0.418
  pilG Neisseria meningitidis 44/76-A

42.092

95.377

0.401
  pilG Neisseria gonorrhoeae MS11

41.944

95.134

0.399
  pilC Thermus thermophilus HB27

37.164

99.513

0.37

Multiple sequence alignment