Detailed information NTG0132726

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OM33_RS13900
• Genome accession: NZ_CP009888
• Coordinates: 3010486..3011370 (+)
• Length: 294 a.a.
• NCBI ID: WP_038642567.1
• Uniprot ID: A0A0A7EHG1
• Organism: Pseudoalteromonas piratica strain OCN003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3005486..3016370

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OM33_RS13880 (OM33_13880)	nadC	3005714..3006568 (+)	855	WP_038642561.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OM33_RS23055	-	3006957..3007082 (+)	126	Protein_2681	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
OM33_RS13890 (OM33_13890)	pilB	3007463..3009151 (+)	1689	WP_038642563.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OM33_RS13895 (OM33_13895)	pilC	3009244..3010479 (+)	1236	WP_038642565.1	type II secretion system F family protein	Machinery gene
OM33_RS13900 (OM33_13900)	pilD	3010486..3011370 (+)	885	WP_038642567.1	A24 family peptidase	Machinery gene
OM33_RS13905 (OM33_13905)	coaE	3011372..3011962 (+)	591	WP_038642569.1	dephospho-CoA kinase	-
OM33_RS13910 (OM33_13910)	pilB	3012094..3013776 (+)	1683	WP_038642571.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OM33_RS13915 (OM33_13915)	yacG	3013860..3014093 (+)	234	WP_038642573.1	DNA gyrase inhibitor YacG	-
OM33_RS13920 (OM33_13920)	-	3014153..3014602 (-)	450	WP_038642575.1	Spy/CpxP family protein refolding chaperone	-
OM33_RS13925 (OM33_13925)	-	3014758..3015441 (+)	684	WP_038642577.1	response regulator transcription factor	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32633.84 Da
• Isoelectric Point: 5.7447

>NTDB_id=132726 OM33_RS13900 WP_038642567.1 3010486..3011370(+) (pilD) [Pseudoalteromonas piratica strain OCN003]
MSSILETFSTTPWFYYLTIFLASICIGSFLNVVIYRLPKMMENEWKAECSLLLADNLKTPYEASSQAFNLITPSSTCPHC
NTKIKPWYNLPVFGWLFLRGKAACCGEKISVRYPSIELLTGLSGLVIAYYFGVTEQALLYLLLTYALITLIFIDIDHMLL
PDQITLPLLWLVLLASVMGLTIDPAQAIIGAVTGYLVFWTVYWVFKLLTGKEGMGYGDFKLMAVFGALLGWQALPMIVLM
SSLVGAVIGITVLSIQGKDKATPIPFGPYIAIAGWISLIWGESIQSTYFNLITP

Nucleotide

• Length: 885 bp

>NTDB_id=132726 OM33_RS13900 WP_038642567.1 3010486..3011370(+) (pilD) [Pseudoalteromonas piratica strain OCN003]
ATGAGCAGTATTTTAGAGACTTTTTCAACAACACCCTGGTTTTATTACCTCACCATATTTTTAGCAAGCATTTGCATTGG
TAGCTTTTTAAATGTAGTGATTTACCGCTTACCTAAAATGATGGAAAACGAATGGAAAGCCGAGTGCAGTTTACTGCTTG
CCGACAACTTAAAAACTCCTTATGAGGCAAGCAGTCAAGCATTTAATTTAATTACACCAAGCTCTACCTGCCCGCATTGC
AATACTAAAATAAAGCCGTGGTATAACTTACCTGTATTTGGCTGGTTATTTTTACGCGGCAAAGCGGCCTGCTGTGGCGA
AAAAATATCAGTGCGCTACCCTAGTATTGAGCTTTTAACTGGCTTATCAGGTTTAGTGATTGCTTATTATTTTGGTGTAA
CTGAACAAGCGCTGCTTTATTTACTACTGACGTATGCCCTAATTACGCTTATCTTCATTGATATAGACCATATGTTACTA
CCAGACCAAATCACCCTACCGTTACTTTGGTTAGTATTATTAGCTTCTGTTATGGGGCTAACGATTGACCCAGCGCAAGC
TATTATTGGCGCTGTAACCGGTTATTTAGTGTTTTGGACTGTTTACTGGGTATTTAAATTACTGACTGGTAAAGAAGGCA
TGGGCTATGGTGATTTTAAGCTAATGGCGGTATTTGGTGCACTTTTAGGCTGGCAAGCGCTACCTATGATTGTGCTGATG
TCGAGCTTAGTCGGTGCCGTGATAGGTATTACAGTGCTTAGTATTCAAGGTAAAGATAAAGCCACTCCCATTCCATTTGG
CCCTTACATTGCCATTGCTGGGTGGATATCACTTATCTGGGGTGAATCGATTCAGTCAACCTATTTTAATTTGATTACCC
CCTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-131)

• Type IV leader peptidase family (Peptidase_A24) (142-250)

3D structure

• AlphaFold DB: A0A0A7EHG1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.523	97.619	0.493
pilD	Acinetobacter baumannii D1279779	49.135	98.299	0.483
pilD	Acinetobacter nosocomialis M2	48.276	98.639	0.476
pilD	Vibrio campbellii strain DS40M4	48.097	98.299	0.473
pilD	Neisseria gonorrhoeae MS11	45.385	88.435	0.401