Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OM33_RS13910 Genome accession   NZ_CP009888
Coordinates   3012094..3013776 (+) Length   560 a.a.
NCBI ID   WP_038642571.1    Uniprot ID   -
Organism   Pseudoalteromonas piratica strain OCN003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3007094..3018776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM33_RS13890 (OM33_13890) pilB 3007463..3009151 (+) 1689 WP_038642563.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM33_RS13895 (OM33_13895) pilC 3009244..3010479 (+) 1236 WP_038642565.1 type II secretion system F family protein Machinery gene
  OM33_RS13900 (OM33_13900) pilD 3010486..3011370 (+) 885 WP_038642567.1 A24 family peptidase Machinery gene
  OM33_RS13905 (OM33_13905) coaE 3011372..3011962 (+) 591 WP_038642569.1 dephospho-CoA kinase -
  OM33_RS13910 (OM33_13910) pilB 3012094..3013776 (+) 1683 WP_038642571.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM33_RS13915 (OM33_13915) yacG 3013860..3014093 (+) 234 WP_038642573.1 DNA gyrase inhibitor YacG -
  OM33_RS13920 (OM33_13920) - 3014153..3014602 (-) 450 WP_038642575.1 Spy/CpxP family protein refolding chaperone -
  OM33_RS13925 (OM33_13925) - 3014758..3015441 (+) 684 WP_038642577.1 response regulator transcription factor -
  OM33_RS13930 (OM33_13930) - 3015441..3016829 (+) 1389 WP_038642579.1 ATP-binding protein -
  OM33_RS13935 (OM33_13935) - 3016813..3017739 (+) 927 WP_038642581.1 alpha/beta fold hydrolase -
  OM33_RS13940 (OM33_13940) - 3017717..3018586 (-) 870 WP_038642583.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61691.59 Da        Isoelectric Point: 5.1180

>NTDB_id=132727 OM33_RS13910 WP_038642571.1 3012094..3013776(+) (pilB) [Pseudoalteromonas piratica strain OCN003]
MNVNSPLIRKCVSLGFIPSDKISTSATANSHTSEFILNNSKLSSKELAKYCSAWFNLPSLDLSKVDPSNVPLDNISEELV
KKHHLIPLCKRGRTLFLATSDPTDFDAFEDFEFNTGLQTEAIVVDYTSLEQFIESIFSKTDDLALSDEEFEQFGDIEVSD
TEANLSEDAAPDKDDAPIIVYINKILMDAIRKGASDLHFEPYEKSYRIRFRIDGILHEVASPPQMLSSRISARVKVMSRL
DIAEKRKPQDGRIKLKITAKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEVEQKAMYMDALSQPQGMILVTG
PTGSGKTVSLYTGLNILNKPEVNISTAEDPVEINLVGVNQVQINPKADMTFANALRAFLRQDPDIVMVGEIRDLETAEIA
IKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCNNCKTPEQLPVEELKSQGFSEQQIASI
TLYKAVGCEHCTEGYKGRVGIYEVMKITPQISQIIMSGGNSLDIAKASLESGFNNLRLSGLEKAAKGVTSLAEINRVTSY

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=132727 OM33_RS13910 WP_038642571.1 3012094..3013776(+) (pilB) [Pseudoalteromonas piratica strain OCN003]
ATGAATGTAAACTCTCCGCTTATTCGAAAGTGTGTTTCTCTTGGTTTTATTCCAAGCGATAAAATATCAACATCTGCAAC
AGCCAATAGCCACACCAGTGAGTTTATTCTTAATAACAGTAAGCTTTCCTCAAAAGAGTTAGCTAAATACTGCTCAGCTT
GGTTTAACCTGCCAAGCCTTGATTTATCAAAAGTCGATCCAAGTAATGTGCCCCTTGATAATATCAGCGAAGAGTTGGTT
AAAAAGCATCATCTTATCCCGCTTTGTAAACGCGGCCGCACGCTCTTTTTAGCCACCTCTGATCCCACCGATTTTGATGC
CTTTGAAGACTTTGAATTTAATACTGGCCTGCAAACCGAAGCCATAGTGGTTGATTACACCTCACTTGAGCAATTTATAG
AGTCAATATTTAGTAAAACCGATGACCTAGCCCTTTCTGATGAGGAGTTTGAACAGTTTGGTGATATTGAAGTAAGCGAT
ACCGAAGCAAACCTATCTGAGGATGCTGCGCCAGACAAAGATGATGCGCCAATTATTGTTTATATCAATAAAATCCTAAT
GGATGCAATTCGCAAAGGCGCATCCGATTTACATTTTGAACCTTATGAAAAATCTTATCGCATTCGCTTTCGTATAGACG
GTATTTTGCATGAAGTAGCATCCCCGCCACAAATGCTCTCGTCGCGTATATCAGCCCGTGTAAAGGTAATGTCACGACTC
GATATTGCCGAAAAACGTAAACCTCAAGATGGCCGCATTAAACTCAAAATAACCGCCAAAAAAAGTATCGATTTTCGTGT
ATCAACCTTGCCGACTCTTTGGGGCGAGAAGATTGTAATGCGTATATTAGATTCTAGTAGCGCCATGTTAGGCATTGATG
TATTAGGCTATGAAGTAGAACAAAAAGCCATGTACATGGATGCTCTGTCACAACCTCAGGGCATGATTTTAGTTACCGGC
CCAACAGGTTCAGGTAAAACAGTATCACTTTACACGGGTTTAAATATTTTAAACAAACCCGAAGTGAATATATCCACAGC
AGAGGATCCCGTCGAAATAAACTTAGTAGGTGTAAACCAAGTACAGATCAACCCCAAAGCTGATATGACATTTGCCAATG
CACTGCGTGCATTTTTACGTCAAGATCCTGATATTGTTATGGTTGGTGAGATCCGTGACTTAGAAACCGCCGAAATTGCC
ATAAAAGCTGCTCAAACCGGTCACTTAGTGCTAAGTACACTTCACACTAACTCAGCACCAGAAACACTTACACGTCTACT
TAATATGGGAGTTCCAGCATACAATGTTGCAAGTTCAATCAGTCTTATCATAGCGCAAAGACTAGCGCGTCGGCTTTGCA
ATAATTGTAAAACACCAGAACAGTTACCCGTGGAAGAGTTAAAGTCACAAGGTTTTTCTGAACAGCAAATTGCTTCTATA
ACCCTATATAAAGCTGTTGGCTGTGAGCACTGCACTGAAGGTTATAAAGGCCGTGTTGGTATCTATGAAGTAATGAAAAT
TACGCCCCAGATTTCACAAATTATTATGTCCGGAGGCAACTCTCTTGATATTGCAAAAGCATCACTTGAAAGTGGTTTTA
ACAATTTAAGATTGTCTGGCTTAGAAAAAGCAGCAAAAGGGGTCACGAGCCTAGCTGAAATCAATCGAGTGACTAGTTAT
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-139)

Type II/IV secretion system protein (T2SSE)

(184-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.704

96.429

0.566
  pilB Acinetobacter baylyi ADP1

56.506

100

0.566
  pilB Legionella pneumophila strain ERS1305867

56.604

94.643

0.536
  pilB Vibrio cholerae strain A1552

53.37

98.036

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

53.484

94.821

0.507
  pilB Vibrio campbellii strain DS40M4

52.166

94.821

0.495
  pilF Neisseria gonorrhoeae MS11

49.237

93.571

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

36.629

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.214

100

0.382
  pilF Thermus thermophilus HB27

36.525

100

0.368

Multiple sequence alignment