Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACG06_RS25520 Genome accession   NZ_CP011857
Coordinates   5486528..5486992 (-) Length   154 a.a.
NCBI ID   WP_058195419.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ATCC 27853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5481528..5491992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG06_RS25505 (ACG06_25525) nadC 5484038..5484886 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACG06_RS25515 (ACG06_25535) - 5485067..5486452 (-) 1386 WP_003122080.1 O-antigen ligase family protein -
  ACG06_RS25520 (ACG06_25540) pilA 5486528..5486992 (-) 465 WP_058195419.1 pilin Machinery gene
  ACG06_RS25525 (ACG06_25545) pilB 5487223..5488923 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG06_RS25530 (ACG06_25550) pilC 5488927..5490144 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  ACG06_RS25535 (ACG06_25555) pilD 5490145..5491017 (+) 873 WP_016852118.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACG06_RS25540 (ACG06_25560) coaE 5491014..5491625 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ACG06_RS25545 (ACG06_25565) yacG 5491622..5491822 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16261.71 Da        Isoelectric Point: 9.4108

>NTDB_id=129452 ACG06_RS25520 WP_058195419.1 5486528..5486992(-) (pilA) [Pseudomonas aeruginosa strain ATCC 27853]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKVTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=129452 ACG06_RS25520 WP_058195419.1 5486528..5486992(-) (pilA) [Pseudomonas aeruginosa strain ATCC 27853]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGTTACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.484

99.351

0.422
  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409
  pilA Vibrio parahaemolyticus RIMD 2210633

39.355

100

0.396
  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37
  pilA Vibrio cholerae C6706

36.774

100

0.37
  pilA Vibrio cholerae strain A1552

36.774

100

0.37
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.37