Detailed information NTG0125682

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ID09_RS00700
• Genome accession: NZ_CP008921
• Coordinates: 104934..105368 (+)
• Length: 144 a.a.
• NCBI ID: WP_024382121.1
• Uniprot ID: A0A075SNP0
• Organism: Streptococcus suis 6407
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99934..110368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ID09_RS00665 (ID09_00680)	-	100676..101041 (+)	366	WP_002936568.1	DUF1033 family protein	-
ID09_RS00670 (ID09_00685)	-	101076..102026 (-)	951	WP_024382126.1	S66 peptidase family protein	-
ID09_RS00675 (ID09_00690)	comYA	102112..103062 (+)	951	WP_024382125.1	competence type IV pilus ATPase ComGA	Machinery gene
ID09_RS00680 (ID09_00695)	comYB	102974..104011 (+)	1038	WP_255195823.1	competence type IV pilus assembly protein ComGB	Machinery gene
ID09_RS00685 (ID09_00700)	comYC	104013..104294 (+)	282	WP_024382124.1	competence type IV pilus major pilin ComGC	Machinery gene
ID09_RS00690 (ID09_00705)	comGD	104275..104682 (+)	408	WP_024382123.1	competence type IV pilus minor pilin ComGD	-
ID09_RS00695 (ID09_00710)	comYE	104654..104947 (+)	294	WP_024382122.1	competence type IV pilus minor pilin ComGE	Machinery gene
ID09_RS00700 (ID09_00715)	comGF/cglF	104934..105368 (+)	435	WP_024382121.1	competence type IV pilus minor pilin ComGF	Machinery gene
ID09_RS00705 (ID09_00720)	comGG	105346..105756 (+)	411	WP_024382120.1	competence type IV pilus minor pilin ComGG	-
ID09_RS00710 (ID09_00725)	comYH	105813..106766 (+)	954	WP_024382119.1	class I SAM-dependent methyltransferase	Machinery gene
ID09_RS00715 (ID09_00730)	-	106816..108003 (+)	1188	WP_038425630.1	acetate kinase	-
ID09_RS00720 (ID09_00735)	-	108318..108869 (+)	552	WP_024382117.1	folate family ECF transporter S component	-
ID09_RS00725 (ID09_00740)	-	108925..110181 (+)	1257	WP_024390768.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16461.82 Da
• Isoelectric Point: 6.9832

>NTDB_id=125682 ID09_RS00700 WP_024382121.1 104934..105368(+) (comGF/cglF) [Streptococcus suis 6407]
MLKSKIPAFTLLECLVALIILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALVFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=125682 ID09_RS00700 WP_024382121.1 104934..105368(+) (comGF/cglF) [Streptococcus suis 6407]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGTCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCTATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAGAACCAGCTGGTGCGCATCGACTTCAGCTTTAAAAACGGAGAGGAGC
GAACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: A0A075SNP0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae TIGR4	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae Rx1	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae D39	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae R6	53.191	97.917	0.521
comGF/cglF	Streptococcus mitis NCTC 12261	53.731	93.056	0.5
comYF	Streptococcus mutans UA140	49.306	100	0.493
comYF	Streptococcus mutans UA159	48.611	100	0.486
comGF/cglF	Streptococcus mitis SK321	51.493	93.056	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	46.809	97.917	0.458