Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C645_RS02015 Genome accession   NZ_CP008740
Coordinates   410039..411433 (-) Length   464 a.a.
NCBI ID   WP_005656265.1    Uniprot ID   A0A0H3PF78
Organism   Haemophilus influenzae 2019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 405039..416433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C645_RS01980 (C645_02005) - 405289..405495 (-) 207 WP_005660027.1 heavy-metal-associated domain-containing protein -
  C645_RS01985 (C645_02010) - 405569..405775 (-) 207 WP_005689491.1 heavy-metal-associated domain-containing protein -
  C645_RS01990 (C645_02015) cueR 405852..406238 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  C645_RS01995 (C645_02020) metJ 406252..406569 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  C645_RS02000 (C645_02025) rho 406817..408079 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  C645_RS02005 (C645_02030) pilD 408133..408825 (-) 693 WP_046067576.1 prepilin peptidase Machinery gene
  C645_RS02010 (C645_02035) pilC 408822..410042 (-) 1221 WP_046067577.1 type II secretion system F family protein Machinery gene
  C645_RS02015 (C645_02040) pilB 410039..411433 (-) 1395 WP_005656265.1 GspE/PulE family protein Machinery gene
  C645_RS02020 (C645_02045) pilA 411430..411879 (-) 450 WP_005656280.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  C645_RS02025 (C645_02050) ampD 411994..412554 (+) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C645_RS02030 (C645_02055) corC 413175..414074 (+) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  C645_RS02035 (C645_02060) lnt 414097..415626 (+) 1530 WP_079997635.1 apolipoprotein N-acyltransferase -
  C645_RS02040 (C645_02065) rsmE 415676..416413 (+) 738 WP_005689496.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52766.20 Da        Isoelectric Point: 6.1416

>NTDB_id=123948 C645_RS02015 WP_005656265.1 410039..411433(-) (pilB) [Haemophilus influenzae 2019]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKC
GGNLANSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDGKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=123948 C645_RS02015 WP_005656265.1 410039..411433(-) (pilB) [Haemophilus influenzae 2019]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTCTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAGCTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGATATTATTATGCTGGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCTATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTCCTACATTGGCA
GCAGAATGGTTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGTATAACAGATGGAA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PF78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

98.272

99.784

0.981
  pilB Haemophilus influenzae 86-028NP

96.983

100

0.97
  pilB Glaesserella parasuis strain SC1401

57.522

97.414

0.56
  pilB Vibrio cholerae strain A1552

39.848

100

0.453
  pilB Vibrio campbellii strain DS40M4

40.9

100

0.431
  pilB Legionella pneumophila strain ERS1305867

39.959

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Acinetobacter baylyi ADP1

39.113

100

0.418
  pilB Acinetobacter baumannii D1279779

39.709

100

0.412
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment