Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   V428_RS20655 Genome accession   NZ_CP007566
Coordinates   4615537..4616778 (+) Length   413 a.a.
NCBI ID   WP_016352169.1    Uniprot ID   -
Organism   Aeromonas hydrophila subsp. hydrophila AL09-71 isolate 4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4610537..4621778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V428_RS23710 (V428_21355) ampD 4610910..4611482 (-) 573 WP_016352163.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V428_RS20635 (V428_21360) - 4611630..4612100 (+) 471 WP_024944772.1 retropepsin-like aspartic protease -
  V428_RS20640 (V428_21365) nadC 4612106..4612963 (+) 858 WP_016352165.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V428_RS23720 tapA 4613286..4613708 (+) 423 WP_016352167.1 type IVa pilus major pilin TapA -
  V428_RS20650 (V428_21375) pilB 4613715..4615421 (+) 1707 WP_016352168.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  V428_RS20655 (V428_21380) pilC 4615537..4616778 (+) 1242 WP_016352169.1 type II secretion system F family protein Machinery gene
  V428_RS20660 (V428_21385) pilD 4616801..4617673 (+) 873 WP_016352170.1 A24 family peptidase Machinery gene
  V428_RS20665 (V428_21390) coaE 4617693..4618307 (+) 615 WP_016352171.1 dephospho-CoA kinase -
  V428_RS20670 (V428_21395) zapD 4618345..4619067 (+) 723 WP_016352172.1 cell division protein ZapD -
  V428_RS20675 (V428_21400) yacG 4619077..4619271 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  V428_RS20680 (V428_21405) mutT 4619332..4619745 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  V428_RS20685 (V428_21410) - 4619754..4620938 (-) 1185 WP_016352174.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45426.54 Da        Isoelectric Point: 9.8734

>NTDB_id=120993 V428_RS20655 WP_016352169.1 4615537..4616778(+) (pilC) [Aeromonas hydrophila subsp. hydrophila AL09-71 isolate 4]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPAFTQFVIGISRFMQNWWYMIFGGVA
LAIFLYVRAWRASQKVRDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=120993 V428_RS20655 WP_016352169.1 4615537..4616778(+) (pilC) [Aeromonas hydrophila subsp. hydrophila AL09-71 isolate 4]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGCGGGGTAAATCGCAAGGGCCA
GAAGGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAACAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCCCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTCCAAAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCGGC
CATGCGCGAGCTCATGGGGCAGATTGCCGCCGATGTGGAGACCGGCACGCCTATGTCCGAGGCACTGCGCCGCCATCCCC
GTCACTTTGACGATCTCTACTGCGATCTGGTGGAGGCTGGCGAACAGTCCGGCGCTCTGGAGACCATCTACGACCGCATC
GCCACCTATCGCGAAAAGTCCGAAGCCCTCAAATCCAAAATCAAGAAAGCCATGTTCTACCCCACCATGGTCATCCTGGT
GGCCATTGTCGTCACCTCCATTCTGCTGCTGTTCGTCATTCCCCAGTTCGAGGACATCTTCAAGAGTTTCGGCGCCGAGC
TGCCCGCTTTTACCCAGTTCGTCATCGGTATCTCCCGCTTCATGCAGAACTGGTGGTATATGATCTTTGGCGGGGTTGCA
CTCGCCATCTTTCTCTATGTGCGCGCCTGGCGCGCCTCACAGAAAGTCAGAGACAACACCGACAAGTTCATACTAACCAT
CCCCGTGGTTGGCATGATATTGCACAAGGCCGCCATGGCCCGTTTCGCCCGTACCCTCTCCACTACCTTCTCCGCCGGTA
TTCCACTGGTGGATGCCTTGGTCTCTGCGGCAGGCGCCTCCGGCAACTATGTCTACCGCACTGCCGTCATGGCCATTCGT
AACGAGGTGGTGGCAGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGAGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAGCAGGAGGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTGGAGCCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGCGGCATGGTGGTCGCT
ATGTATCTACCCATCTTCAAACTGGGGTCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.576

95.884

0.552
  pilC Acinetobacter baumannii D1279779

54.25

96.852

0.525
  pilC Legionella pneumophila strain ERS1305867

51.605

98.063

0.506
  pilC Acinetobacter baylyi ADP1

52.393

96.126

0.504
  pilC Vibrio cholerae strain A1552

47.99

96.368

0.462
  pilC Vibrio campbellii strain DS40M4

47.355

96.126

0.455
  pilG Neisseria meningitidis 44/76-A

40.494

98.063

0.397
  pilG Neisseria gonorrhoeae MS11

40.494

98.063

0.397
  pilC Thermus thermophilus HB27

38

96.852

0.368

Multiple sequence alignment