Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V428_RS20650 Genome accession   NZ_CP007566
Coordinates   4613715..4615421 (+) Length   568 a.a.
NCBI ID   WP_016352168.1    Uniprot ID   -
Organism   Aeromonas hydrophila subsp. hydrophila AL09-71 isolate 4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4608715..4620421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V428_RS20625 (V428_21350) pdhR 4609870..4610634 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  V428_RS23710 (V428_21355) ampD 4610910..4611482 (-) 573 WP_016352163.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V428_RS20635 (V428_21360) - 4611630..4612100 (+) 471 WP_024944772.1 retropepsin-like aspartic protease -
  V428_RS20640 (V428_21365) nadC 4612106..4612963 (+) 858 WP_016352165.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V428_RS23720 tapA 4613286..4613708 (+) 423 WP_016352167.1 type IVa pilus major pilin TapA -
  V428_RS20650 (V428_21375) pilB 4613715..4615421 (+) 1707 WP_016352168.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  V428_RS20655 (V428_21380) pilC 4615537..4616778 (+) 1242 WP_016352169.1 type II secretion system F family protein Machinery gene
  V428_RS20660 (V428_21385) pilD 4616801..4617673 (+) 873 WP_016352170.1 A24 family peptidase Machinery gene
  V428_RS20665 (V428_21390) coaE 4617693..4618307 (+) 615 WP_016352171.1 dephospho-CoA kinase -
  V428_RS20670 (V428_21395) zapD 4618345..4619067 (+) 723 WP_016352172.1 cell division protein ZapD -
  V428_RS20675 (V428_21400) yacG 4619077..4619271 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  V428_RS20680 (V428_21405) mutT 4619332..4619745 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62541.93 Da        Isoelectric Point: 5.4342

>NTDB_id=120992 V428_RS20650 WP_016352168.1 4613715..4615421(+) (pilB) [Aeromonas hydrophila subsp. hydrophila AL09-71 isolate 4]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLTEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=120992 V428_RS20650 WP_016352168.1 4613715..4615421(+) (pilB) [Aeromonas hydrophila subsp. hydrophila AL09-71 isolate 4]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCATCTTCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
TCTGAGCCAGGCCAAGGCACAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CGGACTTCTGCGAGCTGGAGTACGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGACCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTCCCCATCTACACCCAGGGACATACCCTCTATATCGCAATGTCGGA
TCCCACAAATGTGTCCGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AGCTCACCACGGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCTGACGATGACGCACCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGTATTCTGCATGAAATAGCGACGCCACCGGTCAATCTGGCCAATCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCACAGGATGGCCGCATCAAGCTGAAACTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGACTCCTCAG
CGGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATACCTGCGTGCCCTCTCGAAGCCTCAA
GGCATGATCCTGGTGACCGGCCCTACCGGCTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACAGCCGAAGATCCGGTGGAAATCAACCTACCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTACGCTCTTTCTTGCGACAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTAGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAAGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCAGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAATATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTCGAGAAGGCCCGCCTAGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment