Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DI45_RS00650 Genome accession   NZ_CP007560
Coordinates   101927..102865 (+) Length   312 a.a.
NCBI ID   WP_009880361.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS743     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96927..107865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DI45_RS00640 (DI45_00660) rpoC 97676..101317 (+) 3642 WP_011284419.1 DNA-directed RNA polymerase subunit beta' -
  DI45_RS00645 (DI45_00665) - 101469..101834 (+) 366 WP_002986560.1 DUF1033 family protein -
  DI45_RS00650 (DI45_00670) comYA 101927..102865 (+) 939 WP_009880361.1 competence type IV pilus ATPase ComGA Machinery gene
  DI45_RS00655 (DI45_00675) comYB 102801..103835 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DI45_RS00660 (DI45_00680) comGC 103837..104163 (+) 327 WP_012560386.1 competence type IV pilus major pilin ComGC Machinery gene
  DI45_RS00665 (DI45_00685) comGD 104138..104566 (+) 429 WP_047235100.1 competence type IV pilus minor pilin ComGD -
  DI45_RS00670 (DI45_00690) comGE 104523..104807 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  DI45_RS00675 (DI45_00695) comYF 104800..105234 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  DI45_RS00680 (DI45_00700) comGG 105218..105544 (+) 327 WP_009880358.1 competence type IV pilus minor pilin ComGG -
  DI45_RS00685 (DI45_00705) comYH 105642..106595 (+) 954 WP_009880357.1 class I SAM-dependent methyltransferase Machinery gene
  DI45_RS00690 (DI45_00710) - 106654..107850 (+) 1197 WP_030126459.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35017.21 Da        Isoelectric Point: 8.2452

>NTDB_id=120813 DI45_RS00650 WP_009880361.1 101927..102865(+) (comYA) [Streptococcus pyogenes strain NGAS743]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLVSHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHQPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=120813 DI45_RS00650 WP_009880361.1 101927..102865(+) (comYA) [Streptococcus pyogenes strain NGAS743]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTGTTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTCAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCAACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.093

99.679

0.609
  comYA Streptococcus mutans UA140

61.093

99.679

0.609
  comYA Streptococcus gordonii str. Challis substr. CH1

60.129

99.679

0.599
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60

99.359

0.596
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.199

99.679

0.58
  comGA Lactococcus lactis subsp. cremoris KW2

50.161

99.679

0.5
  comGA Latilactobacillus sakei subsp. sakei 23K

39.31

92.949

0.365

Multiple sequence alignment