Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   DI45_RS00675 Genome accession   NZ_CP007560
Coordinates   104800..105234 (+) Length   144 a.a.
NCBI ID   WP_002987783.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS743     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99800..110234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DI45_RS00645 (DI45_00665) - 101469..101834 (+) 366 WP_002986560.1 DUF1033 family protein -
  DI45_RS00650 (DI45_00670) comYA 101927..102865 (+) 939 WP_009880361.1 competence type IV pilus ATPase ComGA Machinery gene
  DI45_RS00655 (DI45_00675) comYB 102801..103835 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DI45_RS00660 (DI45_00680) comGC 103837..104163 (+) 327 WP_012560386.1 competence type IV pilus major pilin ComGC Machinery gene
  DI45_RS00665 (DI45_00685) comGD 104138..104566 (+) 429 WP_047235100.1 competence type IV pilus minor pilin ComGD -
  DI45_RS00670 (DI45_00690) comGE 104523..104807 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  DI45_RS00675 (DI45_00695) comYF 104800..105234 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  DI45_RS00680 (DI45_00700) comGG 105218..105544 (+) 327 WP_009880358.1 competence type IV pilus minor pilin ComGG -
  DI45_RS00685 (DI45_00705) comYH 105642..106595 (+) 954 WP_009880357.1 class I SAM-dependent methyltransferase Machinery gene
  DI45_RS00690 (DI45_00710) - 106654..107850 (+) 1197 WP_030126459.1 acetate kinase -
  DI45_RS00695 (DI45_00715) - 108036..108344 (+) 309 Protein_91 hypothetical protein -
  DI45_RS00700 (DI45_00720) proC 108427..109197 (-) 771 WP_009880354.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16585.23 Da        Isoelectric Point: 10.2064

>NTDB_id=120816 DI45_RS00675 WP_002987783.1 104800..105234(+) (comYF) [Streptococcus pyogenes strain NGAS743]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFCKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=120816 DI45_RS00675 WP_002987783.1 104800..105234(+) (comYF) [Streptococcus pyogenes strain NGAS743]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTACCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTGCAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGATATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment