Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CY96_RS18835 Genome accession   NZ_CP007512
Coordinates   3747637..3748071 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus bombysepticus str. Wang     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3742637..3753071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CY96_RS18815 (CY96_19095) - 3743815..3744372 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  CY96_RS18825 (CY96_19100) metE 3744884..3747172 (+) 2289 WP_001007613.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CY96_RS18830 (CY96_19105) comJ 3747211..3747600 (-) 390 WP_000424030.1 competence protein ComJ -
  CY96_RS18835 (CY96_19110) nucA/comI 3747637..3748071 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  CY96_RS18840 (CY96_19115) - 3748298..3749047 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  CY96_RS18845 (CY96_19120) kinB 3749086..3750360 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  CY96_RS18850 (CY96_19125) - 3750671..3750871 (+) 201 WP_000929280.1 hypothetical protein -
  CY96_RS18855 (CY96_19130) - 3750994..3752157 (+) 1164 WP_000434600.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=120214 CY96_RS18835 WP_000811494.1 3747637..3748071(-) (nucA/comI) [Bacillus bombysepticus str. Wang]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=120214 CY96_RS18835 WP_000811494.1 3747637..3748071(-) (nucA/comI) [Bacillus bombysepticus str. Wang]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCGCCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment