Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   RR32_RS01670 Genome accession   NZ_CP010368
Coordinates   364295..364768 (+) Length   157 a.a.
NCBI ID   WP_002051636.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain 6411     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359295..369768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR32_RS01650 (RR32_01655) - 360687..361574 (+) 888 WP_002051771.1 metal-dependent hydrolase -
  RR32_RS01655 (RR32_01660) - 361707..362297 (+) 591 WP_002051712.1 LemA family protein -
  RR32_RS01660 (RR32_01665) - 362319..363401 (+) 1083 WP_004884716.1 TPM domain-containing protein -
  RR32_RS01665 (RR32_01670) - 363395..363955 (+) 561 WP_032011720.1 TPM domain-containing protein -
  RR32_RS01670 (RR32_01675) pilA2 364295..364768 (+) 474 WP_002051636.1 pilin Machinery gene
  RR32_RS01675 (RR32_01680) - 364870..365094 (+) 225 WP_002051650.1 hypothetical protein -
  RR32_RS01680 (RR32_01685) tfpZ 365123..365857 (+) 735 WP_002051776.1 TfpX/TfpZ family type IV pilin accessory protein -
  RR32_RS01685 (RR32_01690) - 365956..367584 (+) 1629 WP_039246139.1 PglL family O-oligosaccharyltransferase -
  RR32_RS01690 (RR32_01695) bfr 367625..368089 (-) 465 WP_039246140.1 heteropolymeric bacterioferritin subunit Bfr -
  RR32_RS01695 (RR32_01700) - 368333..368527 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  RR32_RS01700 (RR32_01705) - 368747..369130 (-) 384 WP_002051728.1 RidA family protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16245.55 Da        Isoelectric Point: 4.9619

>NTDB_id=120214 RR32_RS01670 WP_002051636.1 364295..364768(+) (pilA2) [Acinetobacter nosocomialis strain 6411]
MNAQKGFTLIELMIVVAIIGILAAIALPAYQTYMVKSKLVEATTDLDAAKVAVAEAYSTNDNQFPLTANSPIPPLGANAK
YVTALTYTGTANGPITIVATLGGTNNTKVDGKFIGLTGTGQADGTVSWSCSTFTSTTKAGADTELYPFLPTNCQKAS

Nucleotide


Download         Length: 474 bp        

>NTDB_id=120214 RR32_RS01670 WP_002051636.1 364295..364768(+) (pilA2) [Acinetobacter nosocomialis strain 6411]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTCATGATCGTTGTAGCGATTATTGGTATTTTGGCTGCGATTGCTTT
ACCAGCTTATCAAACTTATATGGTCAAATCTAAGTTGGTAGAGGCAACAACTGATTTAGATGCTGCAAAAGTAGCTGTTG
CAGAAGCTTACTCTACAAATGATAATCAATTTCCATTAACAGCAAATAGTCCTATCCCACCTTTAGGTGCTAATGCTAAA
TATGTTACGGCATTAACTTATACTGGAACAGCTAATGGTCCAATCACAATTGTTGCGACTCTTGGTGGTACAAACAATAC
AAAGGTTGATGGTAAATTTATTGGTTTGACTGGTACTGGTCAGGCTGATGGAACAGTTTCATGGTCATGTAGTACATTTA
CAAGTACAACGAAAGCAGGTGCAGACACAGAACTATATCCATTCTTACCAACTAATTGTCAAAAGGCAAGTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

40.964

100

0.433
  pilA2 Legionella pneumophila str. Paris

42.484

97.452

0.414
  pilA2 Legionella pneumophila strain ERS1305867

42.484

97.452

0.414
  comP Acinetobacter baylyi ADP1

37.423

100

0.389
  pilA Acinetobacter baumannii strain A118

37.975

100

0.382
  pilE Neisseria gonorrhoeae strain FA1090

36.585

100

0.382
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.165

88.535

0.382
  pilE Neisseria gonorrhoeae MS11

41.259

91.083

0.376
  pilA Ralstonia pseudosolanacearum GMI1000

35.583

100

0.369
  pilA Haemophilus influenzae 86-028NP

38.926

94.904

0.369

Multiple sequence alignment