Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NTHIC486_RS03445 Genome accession   NZ_CP007471
Coordinates   675844..677241 (+) Length   465 a.a.
NCBI ID   WP_005686724.1    Uniprot ID   -
Organism   Haemophilus influenzae strain C486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 670844..682241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHIC486_RS03420 (NTHIC486_00684) rsmE 670862..671599 (-) 738 WP_044345403.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NTHIC486_RS03425 (NTHIC486_00685) lnt 671649..673178 (-) 1530 WP_080334644.1 apolipoprotein N-acyltransferase -
  NTHIC486_RS03430 (NTHIC486_00686) corC 673201..674100 (-) 900 WP_044345407.1 CNNM family magnesium/cobalt transport protein CorC -
  NTHIC486_RS03435 (NTHIC486_00687) ampD 674723..675283 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NTHIC486_RS03440 (NTHIC486_00688) pilA 675398..675847 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NTHIC486_RS03445 (NTHIC486_00689) pilB 675844..677241 (+) 1398 WP_005686724.1 GspE/PulE family protein Machinery gene
  NTHIC486_RS03450 (NTHIC486_00690) pilC 677238..678455 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  NTHIC486_RS03455 (NTHIC486_00691) pilD 678452..679144 (+) 693 WP_005686720.1 prepilin peptidase Machinery gene
  NTHIC486_RS03460 (NTHIC486_00692) rho 679198..680460 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  NTHIC486_RS03465 (NTHIC486_00693) metJ 680708..681025 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  NTHIC486_RS03470 (NTHIC486_00694) cueR 681039..681425 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  NTHIC486_RS03475 (NTHIC486_00695) - 681502..681708 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03480 (NTHIC486_00696) - 681794..682000 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53061.39 Da        Isoelectric Point: 5.6399

>NTDB_id=119745 NTHIC486_RS03445 WP_005686724.1 675844..677241(+) (pilB) [Haemophilus influenzae strain C486]
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RAFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=119745 NTHIC486_RS03445 WP_005686724.1 675844..677241(+) (pilB) [Haemophilus influenzae strain C486]
ATGACGAGTTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGTCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTTCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGAGGAAGAGCAAGTTAAATATTA
CCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTG
CCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTT
TTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATAT
TAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAA
CCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTG
GGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCCAC
AGGAAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACACCTGATAAACATATTATGACTGCTGAGG
ATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTG
CGCGCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGCATTACG
TGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGTTTACAACAAC
TCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAG
TGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTG
GCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATG
AGAAAGAAATTGAACGTGTGTTAGGTAAAAACTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-98)

Type II/IV secretion system protein (T2SSE)

(129-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.198

99.785

0.97
  pilB Haemophilus influenzae Rd KW20

95.269

100

0.953
  pilB Glaesserella parasuis strain SC1401

58.297

98.495

0.574
  pilB Vibrio cholerae strain A1552

40.607

100

0.46
  pilB Vibrio campbellii strain DS40M4

40.9

100

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

40.58

100

0.422
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.415
  pilB Acinetobacter baylyi ADP1

38.384

100

0.409
  pilB Acinetobacter baumannii D1279779

38.763

100

0.404
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.953

100

0.383
  pilF Thermus thermophilus HB27

36

100

0.368

Multiple sequence alignment