Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RN69_RS17715 Genome accession   NZ_CP010313
Coordinates   3791345..3793042 (+) Length   565 a.a.
NCBI ID   WP_014493806.1    Uniprot ID   -
Organism   Bradyrhizobium japonicum strain E109     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3786345..3798042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN69_RS17700 (RN69_17825) - 3787539..3788000 (+) 462 WP_014493803.1 tetratricopeptide repeat protein -
  RN69_RS17705 (RN69_17830) - 3788134..3788670 (-) 537 WP_202556875.1 prepilin peptidase -
  RN69_RS17710 (RN69_17835) gspD 3788838..3791117 (-) 2280 WP_014493805.1 type II secretion system secretin GspD -
  RN69_RS17715 (RN69_17840) pilB 3791345..3793042 (+) 1698 WP_014493806.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  RN69_RS17720 (RN69_17845) - 3793046..3794257 (+) 1212 WP_014493807.1 type II secretion system F family protein -
  RN69_RS17725 (RN69_17850) gspG 3794281..3794736 (+) 456 WP_014493808.1 type II secretion system major pseudopilin GspG -
  RN69_RS17730 (RN69_17855) - 3794699..3795223 (+) 525 WP_014493809.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RN69_RS17735 (RN69_17860) - 3795192..3795596 (+) 405 WP_240537981.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RN69_RS17740 (RN69_17865) - 3795623..3796327 (+) 705 WP_225894834.1 general secretion pathway protein GspJ -
  RN69_RS17745 (RN69_17870) - 3796324..3797238 (+) 915 WP_014493812.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61344.46 Da        Isoelectric Point: 6.4564

>NTDB_id=119746 RN69_RS17715 WP_014493806.1 3791345..3793042(+) (pilB) [Bradyrhizobium japonicum strain E109]
MNAMRDRSADSFRQHLLEKYSLPLRAHAHVDKSANPAITRPLRELWEATDLSAADFADEVSDYFALPRLSLPQLLAATPR
LDGFSRRFLRESTIFPFSATSDVFRLAVADPSDTAAIRAAEIVFGTPVDVVVASYEDITTVLDQRTEAGDAKAEDGVRSI
AQQSDDDIESLRDLASGAPVVRALNDLLERAVELRASDIHVEPFRAGLTVRMRVDGLLRALPSPHGIPPQALISRIKILA
SLNIAERRLPQDGAARVRVGRGEIDVRVATMPTQHGESAVIRLLPRDRGLLEMGKLGLAAHDERAMTRLLAMPHGMIVIT
GPTGSGKTTTLATMLSILNEPTRKILTIEDPVEYEIPGINQSQVKPSIGLTFASAMRAFVRQDPDVIMVGEVRDAETAHI
AIHAALTGHLVLTTLHTETAAAAVPRLIDLGIEGFLLKSTLRAVVAQRLVRMLCDRCKVPHTLTEADLAKDPRFAVIGFK
CGEVVHEAGGCERCGGTGYRGRNGVFEILEMSDEVRTLIGPQTDSHSIDAAAMRGGMTTMLEDAVAKCRAGLTTVPEVFR
VTTVR

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=119746 RN69_RS17715 WP_014493806.1 3791345..3793042(+) (pilB) [Bradyrhizobium japonicum strain E109]
GTGAATGCGATGCGCGACCGCTCCGCGGATAGCTTCCGGCAGCACCTCCTGGAAAAATATTCACTGCCGCTGCGGGCGCA
CGCCCATGTGGACAAGTCAGCCAATCCCGCGATTACGCGCCCCTTGCGCGAATTGTGGGAGGCCACCGACCTTTCCGCGG
CCGATTTCGCCGATGAAGTATCCGACTATTTTGCACTGCCGCGGCTGAGCCTTCCGCAACTGCTCGCGGCTACACCTCGT
CTCGACGGCTTTTCGCGCCGTTTCCTGCGTGAATCCACGATCTTTCCCTTCAGTGCGACGAGTGACGTTTTCAGATTGGC
TGTTGCCGATCCTTCCGACACCGCTGCCATTCGCGCGGCCGAAATCGTCTTCGGAACGCCGGTCGATGTCGTCGTGGCGT
CATATGAGGACATTACGACGGTACTCGATCAGCGCACCGAGGCGGGCGATGCGAAGGCCGAGGACGGCGTGCGCAGCATC
GCGCAGCAGTCCGACGACGACATCGAGAGCCTGCGCGATCTCGCCAGCGGTGCGCCGGTGGTGCGCGCGCTCAACGATCT
CCTCGAGCGTGCGGTGGAATTGCGCGCCAGCGACATTCATGTCGAGCCGTTCCGCGCCGGGCTCACGGTGCGCATGCGCG
TGGATGGCCTGTTGCGCGCGCTGCCGTCGCCGCACGGCATTCCGCCGCAGGCGCTGATCTCGCGCATCAAGATTCTCGCC
AGCCTCAACATCGCGGAACGGCGGCTGCCGCAGGACGGCGCCGCGCGCGTGCGCGTCGGGCGCGGCGAGATCGACGTCCG
CGTCGCGACCATGCCGACGCAGCATGGCGAGAGCGCCGTCATCCGCCTGCTGCCGCGCGACCGCGGCCTGCTGGAAATGG
GCAAGCTCGGCCTTGCCGCGCACGACGAGAGGGCGATGACGCGGCTGCTCGCGATGCCGCACGGCATGATAGTGATCACC
GGCCCGACCGGCAGCGGCAAGACCACGACGCTCGCCACCATGCTGTCGATCCTCAACGAACCGACGCGCAAGATCCTGAC
CATCGAGGATCCCGTCGAATACGAGATCCCCGGCATCAACCAGTCCCAGGTCAAGCCGTCGATCGGACTGACCTTCGCCT
CCGCCATGCGCGCCTTCGTGCGCCAGGACCCCGACGTCATCATGGTCGGCGAGGTCCGCGACGCCGAGACCGCTCATATC
GCGATCCATGCCGCGCTGACCGGCCATCTCGTGCTGACGACGCTGCACACCGAGACCGCGGCCGCCGCCGTGCCGCGCCT
GATCGATCTCGGCATCGAGGGCTTCCTGCTCAAGTCCACGCTGCGCGCGGTGGTCGCGCAGCGCCTGGTACGCATGCTCT
GCGACCGCTGCAAGGTGCCGCACACGCTGACCGAGGCCGATCTCGCCAAGGACCCGCGCTTTGCGGTGATCGGATTCAAG
TGCGGCGAGGTCGTGCACGAGGCCGGCGGCTGCGAACGCTGCGGCGGCACCGGCTATCGCGGGCGCAACGGCGTGTTCGA
GATCCTGGAGATGTCCGACGAGGTGCGCACGCTGATCGGCCCGCAGACCGACTCCCATTCGATCGATGCCGCCGCGATGC
GCGGCGGCATGACGACGATGCTGGAGGATGCGGTCGCCAAATGCCGGGCCGGGCTGACGACGGTCCCCGAAGTCTTCCGC
GTCACGACGGTGCGCTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(187-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

39.286

94.159

0.37

Multiple sequence alignment