Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NTHIC486_RS03450 Genome accession   NZ_CP007471
Coordinates   677238..678455 (+) Length   405 a.a.
NCBI ID   WP_005686722.1    Uniprot ID   -
Organism   Haemophilus influenzae strain C486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672238..683455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHIC486_RS03430 (NTHIC486_00686) corC 673201..674100 (-) 900 WP_044345407.1 CNNM family magnesium/cobalt transport protein CorC -
  NTHIC486_RS03435 (NTHIC486_00687) ampD 674723..675283 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NTHIC486_RS03440 (NTHIC486_00688) pilA 675398..675847 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NTHIC486_RS03445 (NTHIC486_00689) pilB 675844..677241 (+) 1398 WP_005686724.1 GspE/PulE family protein Machinery gene
  NTHIC486_RS03450 (NTHIC486_00690) pilC 677238..678455 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  NTHIC486_RS03455 (NTHIC486_00691) pilD 678452..679144 (+) 693 WP_005686720.1 prepilin peptidase Machinery gene
  NTHIC486_RS03460 (NTHIC486_00692) rho 679198..680460 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  NTHIC486_RS03465 (NTHIC486_00693) metJ 680708..681025 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  NTHIC486_RS03470 (NTHIC486_00694) cueR 681039..681425 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  NTHIC486_RS03475 (NTHIC486_00695) - 681502..681708 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03480 (NTHIC486_00696) - 681794..682000 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03485 (NTHIC486_00697) - 682086..682292 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03490 (NTHIC486_00698) - 682378..682584 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 46122.32 Da        Isoelectric Point: 9.7232

>NTDB_id=119746 NTHIC486_RS03450 WP_005686722.1 677238..678455(+) (pilC) [Haemophilus influenzae strain C486]
MTKKLFYYQGSNALNQKQKGSIIADTKQQAHFQLISRELTHIKLQQNWQFGAKPKNSEISELLNQLATLLQSAIPLKNSL
QILQQNCTQIMLNEWLERLLQSIESGLAFSQAIEQQGKYLTQQEIQLIQVGEMTGKLAVVCKKIATHRSQSLALQRKLQK
IMLYPSMVLGISLLLTLALLLFIVPQFAEMYSGNNAELPTITAILLSISNFLKQNIGILLFFALSFVLFYYFYLKRQIWF
HQKKNQLISVTPIFGTIQKLSRLVNFSQSLQIMLQAGVPLNQALDSFLPRTQTWQTKTLVNDIILDKEVRSILQWVSQGY
AFSNSVSSDLFPMEAQQMLQIGEQSGKLALMLEHIADNYQEKLNHQIDLLSQMLEPLMMVIIGSLIGIIMMGMYLPIFNM
GSVIQ

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=119746 NTHIC486_RS03450 WP_005686722.1 677238..678455(+) (pilC) [Haemophilus influenzae strain C486]
ATGACTAAAAAACTCTTTTATTATCAAGGTAGTAACGCATTAAATCAGAAACAAAAAGGCTCAATTATTGCGGATACGAA
ACAACAAGCACACTTTCAGTTAATAAGCCGCGAGCTTACTCACATCAAATTACAACAAAACTGGCAATTTGGGGCAAAGC
CCAAAAATTCAGAAATCAGTGAATTACTCAATCAATTAGCGACATTGCTACAGTCCGCAATTCCGTTAAAAAACAGCCTG
CAAATTTTGCAACAAAATTGTACTCAAATTATGCTCAACGAATGGCTTGAACGACTGCTTCAATCCATTGAATCTGGCTT
AGCATTCTCACAAGCAATTGAACAACAAGGAAAATATCTCACACAACAAGAAATTCAACTGATTCAAGTGGGAGAAATGA
CAGGAAAACTTGCCGTAGTTTGTAAAAAAATAGCCACGCACCGTAGCCAATCTTTAGCATTACAACGCAAATTACAGAAA
ATTATGTTATATCCCTCAATGGTATTGGGAATTTCTCTATTATTGACACTCGCATTACTGCTTTTTATCGTGCCTCAATT
TGCTGAAATGTACAGTGGCAACAATGCCGAATTACCAACAATAACTGCAATATTGCTCTCTATATCCAATTTTCTTAAGC
AAAATATTGGCATTTTGCTATTTTTCGCTTTGAGTTTTGTTCTATTTTATTACTTCTATCTAAAACGCCAGATCTGGTTT
CATCAAAAGAAAAATCAACTTATCTCTGTCACGCCTATTTTTGGCACAATTCAAAAACTTTCTCGTTTAGTGAACTTTAG
TCAAAGTTTACAAATTATGTTGCAGGCCGGCGTACCGCTTAATCAAGCACTAGACAGTTTTCTTCCTCGCACACAAACGT
GGCAAACCAAGACGCTTGTAAATGACATCATATTAGATAAAGAAGTGCGGTCAATTTTACAATGGGTTTCTCAAGGCTAT
GCGTTTTCTAATAGCGTAAGTAGCGATCTTTTCCCGATGGAAGCACAACAAATGTTACAAATTGGCGAGCAAAGCGGAAA
ACTCGCTTTGATGCTAGAGCATATTGCGGATAATTACCAGGAAAAACTTAATCATCAAATTGACTTACTCTCACAAATGC
TAGAACCATTAATGATGGTAATTATCGGCAGTCTGATTGGAATTATTATGATGGGAATGTATTTACCTATCTTTAATATG
GGCTCTGTTATTCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(266-396)

Type II secretion system (T2SS), protein F (T2SSF)

(63-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Haemophilus influenzae 86-028NP

97.291

100

0.975
  pilC Haemophilus influenzae Rd KW20

97.044

100

0.973
  pilC Glaesserella parasuis strain SC1401

38.25

98.765

0.378

Multiple sequence alignment