Detailed information NTG1171297

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DYB47_RS01365
• Genome accession: NZ_UHFV01000002
• Coordinates: 235696..236127 (+)
• Length: 143 a.a.
• NCBI ID: WP_003079210.1
• Uniprot ID: G5JWB2
• Organism: Streptococcus macacae NCTC 11558
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 230696..241127

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DYB47_RS01335 (NCTC11558_00270)	-	232360..232740 (+)	381	WP_003081066.1	DUF1033 family protein	-
DYB47_RS01340 (NCTC11558_00271)	comYA	232834..233775 (+)	942	WP_003079951.1	competence type IV pilus ATPase ComGA	Machinery gene
DYB47_RS01345 (NCTC11558_00272)	comYB	233699..234742 (+)	1044	WP_081479323.1	competence type IV pilus assembly protein ComGB	Machinery gene
DYB47_RS01350 (NCTC11558_00273)	comYC	234742..235056 (+)	315	WP_003080278.1	competence type IV pilus major pilin ComGC	Machinery gene
DYB47_RS01355 (NCTC11558_00274)	comYD	235043..235462 (+)	420	WP_003082165.1	competence type IV pilus minor pilin ComGD	Machinery gene
DYB47_RS01360	comYE	235416..235715 (+)	300	WP_040841662.1	competence type IV pilus minor pilin ComGE	Machinery gene
DYB47_RS01365 (NCTC11558_00276)	comYF	235696..236127 (+)	432	WP_003079210.1	competence type IV pilus minor pilin ComGF	Machinery gene
DYB47_RS09855 (NCTC11558_00277)	comYG	236108..236485 (+)	378	WP_040841664.1	competence type IV pilus minor pilin ComGG	Machinery gene
DYB47_RS01375 (NCTC11558_00278)	comYH	236544..237503 (+)	960	WP_003080448.1	class I SAM-dependent methyltransferase	Machinery gene
DYB47_RS01380 (NCTC11558_00279)	-	237558..238754 (+)	1197	WP_003081585.1	acetate kinase	-
DYB47_RS01385 (NCTC11558_00280)	-	238981..239658 (+)	678	WP_003080765.1	CPBP family intramembrane glutamic endopeptidase	-
DYB47_RS01390 (NCTC11558_00281)	proC	239749..240519 (-)	771	WP_003080000.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 143 a.a.
• Molecular weight: 16376.75 Da
• Isoelectric Point: 6.9560

>NTDB_id=1171297 DYB47_RS01365 WP_003079210.1 235696..236127(+) (comYF) [Streptococcus macacae NCTC 11558]
MLKRSLKAFTLLECLVALLTIAGSISIYNGLTKIVSSNVHYLSNNEESDWLLFCQQFRSELEETTFQRIEDGKLYVKKDN
KELAFGKSRSTDFRKTNADGRGYQPMLLGIKEATISQSAKIVRVDIVFENGLERTFMYAFEEN

Nucleotide

• Length: 432 bp

>NTDB_id=1171297 DYB47_RS01365 WP_003079210.1 235696..236127(+) (comYF) [Streptococcus macacae NCTC 11558]
TTGTTAAAAAGATCCCTTAAAGCCTTCACACTTTTAGAATGCTTAGTTGCTTTGTTAACAATAGCAGGAAGTATTAGTAT
TTATAATGGATTGACAAAAATAGTTAGCAGTAATGTACATTACCTATCTAATAATGAAGAAAGTGATTGGCTGCTATTTT
GCCAACAATTTCGTTCAGAACTTGAAGAGACAACTTTTCAAAGAATAGAGGATGGAAAGCTATATGTCAAAAAAGATAAT
AAGGAGCTGGCGTTTGGTAAGTCAAGGTCAACAGATTTTAGGAAAACAAATGCTGATGGAAGGGGGTATCAACCTATGTT
ATTAGGGATAAAAGAGGCAACTATCAGTCAATCAGCTAAAATTGTGAGAGTTGACATTGTATTTGAAAATGGATTAGAGA
GGACCTTTATGTATGCTTTTGAGGAAAATTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (36-133)

3D structure

• AlphaFold DB: G5JWB2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	69.718	99.301	0.692
comYF	Streptococcus mutans UA159	69.014	99.301	0.685
comGF/cglF	Streptococcus mitis NCTC 12261	45.985	95.804	0.441
comGF/cglF	Streptococcus pneumoniae Rx1	45.255	95.804	0.434
comGF/cglF	Streptococcus pneumoniae D39	45.255	95.804	0.434
comGF/cglF	Streptococcus pneumoniae R6	45.255	95.804	0.434
comGF/cglF	Streptococcus pneumoniae TIGR4	45.255	95.804	0.434
comGF/cglF	Streptococcus mitis SK321	44.526	95.804	0.427
comGF	Lactococcus lactis subsp. cremoris KW2	43.262	98.601	0.427