Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbB/cilA   Type   Machinery gene
Locus tag   ACLV7W_RS08225 Genome accession   NZ_OZ217346
Coordinates   1705501..1705896 (-) Length   131 a.a.
NCBI ID   WP_173875848.1    Uniprot ID   A0A6M9F861
Organism   Streptococcus mitis isolate S. mitis D22     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1701201..1749173 1705501..1705896 within 0


Gene organization within MGE regions


Location: 1701201..1749173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7W_RS08205 (SMID22_16910) - 1701348..1702121 (-) 774 WP_045612826.1 class I SAM-dependent methyltransferase -
  ACLV7W_RS08210 (SMID22_16920) - 1702521..1703288 (-) 768 WP_020900987.1 epoxyqueuosine reductase QueH -
  ACLV7W_RS08215 (SMID22_16930) groL 1703423..1705045 (-) 1623 WP_411865769.1 chaperonin GroEL -
  ACLV7W_RS08220 (SMID22_16940) groES 1705061..1705345 (-) 285 WP_000917335.1 co-chaperone GroES -
  ACLV7W_RS08225 (SMID22_16950) ssbB/cilA 1705501..1705896 (-) 396 WP_173875848.1 single-stranded DNA-binding protein Machinery gene
  ACLV7W_RS08230 (SMID22_16960) - 1705974..1706735 (-) 762 WP_173875847.1 SDR family NAD(P)-dependent oxidoreductase -
  ACLV7W_RS08235 (SMID22_16970) ytpR 1706777..1707403 (-) 627 WP_094753572.1 YtpR family tRNA-binding protein -
  ACLV7W_RS08240 (SMID22_16980) - 1707419..1707736 (-) 318 WP_094753573.1 thioredoxin family protein -
  ACLV7W_RS08245 (SMID22_16990) - 1707733..1708032 (-) 300 WP_153209849.1 DUF4651 domain-containing protein -
  ACLV7W_RS08250 (SMID22_17000) - 1708123..1709196 (-) 1074 WP_411865770.1 N-acetylmuramoyl-L-alanine amidase family protein -
  ACLV7W_RS08255 (SMID22_17010) - 1709316..1710923 (-) 1608 WP_049493610.1 CTP synthase -
  ACLV7W_RS08260 (SMID22_17020) - 1711502..1712839 (-) 1338 WP_411865771.1 ISLre2 family transposase -
  ACLV7W_RS08265 (SMID22_17030) - 1712950..1713279 (-) 330 WP_411865772.1 hypothetical protein -
  ACLV7W_RS08270 (SMID22_17040) - 1713297..1713677 (-) 381 WP_411865773.1 replication initiator protein A -
  ACLV7W_RS08275 (SMID22_17050) - 1713677..1713865 (-) 189 WP_049539904.1 hypothetical protein -
  ACLV7W_RS08280 (SMID22_17060) - 1713892..1714164 (-) 273 WP_411865774.1 hypothetical protein -
  ACLV7W_RS08285 (SMID22_17070) - 1714161..1714979 (-) 819 WP_411865775.1 ParA family protein -
  ACLV7W_RS08290 (SMID22_17080) - 1715103..1715693 (-) 591 WP_411865776.1 hypothetical protein -
  ACLV7W_RS08295 (SMID22_17090) - 1715708..1717048 (-) 1341 WP_411865777.1 CHAP domain-containing protein -
  ACLV7W_RS08300 (SMID22_17100) - 1717064..1717315 (-) 252 WP_411865778.1 hypothetical protein -
  ACLV7W_RS08305 (SMID22_17110) - 1717335..1719284 (-) 1950 WP_411865779.1 virulence factor -
  ACLV7W_RS08310 (SMID22_17120) - 1719295..1719942 (-) 648 WP_411865780.1 hypothetical protein -
  ACLV7W_RS08315 (SMID22_17130) - 1719942..1720250 (-) 309 WP_049538583.1 DUF5592 family protein -
  ACLV7W_RS08320 (SMID22_17140) - 1720414..1721544 (-) 1131 WP_261085707.1 helix-turn-helix domain-containing protein -
  ACLV7W_RS08325 (SMID22_17150) - 1721525..1722196 (-) 672 WP_411865781.1 hypothetical protein -
  ACLV7W_RS08330 (SMID22_17160) - 1722302..1722583 (-) 282 WP_411865782.1 BRCT domain-containing protein -
  ACLV7W_RS08335 (SMID22_17170) - 1722592..1724913 (-) 2322 WP_411865941.1 pLS20_p028 family conjugation system transmembrane protein -
  ACLV7W_RS08340 (SMID22_17180) - 1725035..1725532 (-) 498 WP_411865783.1 lipoprotein -
  ACLV7W_RS08345 (SMID22_17190) - 1725758..1726255 (-) 498 WP_411865784.1 hypothetical protein -
  ACLV7W_RS08350 (SMID22_17200) - 1726341..1729469 (-) 3129 WP_411865785.1 VirD4-like conjugal transfer protein, CD1115 family -
  ACLV7W_RS08355 (SMID22_17210) - 1729474..1729719 (-) 246 WP_411865786.1 hypothetical protein -
  ACLV7W_RS08360 (SMID22_17220) - 1729891..1730328 (-) 438 WP_411865787.1 single-stranded DNA-binding protein -
  ACLV7W_RS08365 (SMID22_17230) - 1730405..1733467 (-) 3063 WP_411865788.1 SspB-related isopeptide-forming adhesin -
  ACLV7W_RS08370 (SMID22_17240) - 1733491..1734303 (-) 813 WP_411865789.1 LPXTG cell wall anchor domain-containing protein -
  ACLV7W_RS08375 (SMID22_17250) - 1734320..1735393 (-) 1074 WP_411865790.1 hypothetical protein -
  ACLV7W_RS08380 (SMID22_17260) - 1735406..1735744 (-) 339 WP_125447545.1 conjugal transfer protein TrbC -
  ACLV7W_RS08385 (SMID22_17270) - 1735835..1737685 (-) 1851 WP_411865791.1 AAA family ATPase -
  ACLV7W_RS08390 (SMID22_17280) - 1737688..1739433 (-) 1746 WP_411865792.1 type IA DNA topoisomerase -
  ACLV7W_RS08395 (SMID22_17290) - 1739595..1744448 (-) 4854 WP_411865793.1 PBECR4 domain-containing protein -
  ACLV7W_RS08400 (SMID22_17300) - 1744460..1744720 (-) 261 WP_411865794.1 hypothetical protein -
  ACLV7W_RS08405 (SMID22_17310) mobP2 1744838..1747207 (-) 2370 WP_411865795.1 MobP2 family relaxase -
  ACLV7W_RS08410 (SMID22_17320) - 1747226..1747786 (-) 561 WP_411865796.1 hypothetical protein -
  ACLV7W_RS08415 (SMID22_17330) - 1747797..1748246 (-) 450 WP_125447557.1 hypothetical protein -
  ACLV7W_RS08420 (SMID22_17340) - 1748257..1749045 (-) 789 WP_411865797.1 DNA/RNA non-specific endonuclease -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14925.93 Da        Isoelectric Point: 7.0191

>NTDB_id=1170455 ACLV7W_RS08225 WP_173875848.1 1705501..1705896(-) (ssbB/cilA) [Streptococcus mitis isolate S. mitis D22]
MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVNMVLWGRLAETLASYATKGSLISVDGELRTR
RFEKNGQMNYVTEVLVTGFQLLESRAQRAMRENNAGKDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=1170455 ACLV7W_RS08225 WP_173875848.1 1705501..1705896(-) (ssbB/cilA) [Streptococcus mitis isolate S. mitis D22]
ATGTATAATAAAGTTATCATGATTGGACGTTTAACGTCTACACCAGAATTGCACAAAACCAATAATGACAAGTCAGTAGC
GCGCGCAACGATCGCTGTGAACCGTCGTTACAAAGACCAAAACGGTGAACGCGAAGCTGATTTTGTTAATATGGTTTTAT
GGGGTAGACTAGCAGAAACTTTGGCAAGCTACGCAACCAAAGGTAGTCTTATTTCTGTGGATGGAGAATTACGTACCCGT
CGTTTTGAGAAAAACGGTCAGATGAACTACGTGACCGAAGTCCTTGTCACAGGATTCCAACTCTTGGAAAGTCGTGCCCA
ACGTGCTATGCGAGAAAATAACGCAGGGAAGGATTTGGCAGATTTGGTTTTGGAAGAGGAAGAATTGCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M9F861

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus pneumoniae D39

99.237

100

0.992
  ssbB/cilA Streptococcus pneumoniae Rx1

99.237

100

0.992
  ssbB/cilA Streptococcus pneumoniae R6

99.237

100

0.992
  ssbB/cilA Streptococcus pneumoniae TIGR4

98.473

100

0.985
  ssbB/cilA Streptococcus mitis SK321

97.71

100

0.977
  ssbB/cilA Streptococcus mitis NCTC 12261

97.71

100

0.977
  ssbA Streptococcus mutans UA159

74.809

100

0.748
  ssbB Streptococcus sobrinus strain NIDR 6715-7

70.229

100

0.702
  ssbB Lactococcus lactis subsp. cremoris KW2

59.821

85.496

0.511
  ssbA Bacillus subtilis subsp. subtilis str. 168

50.943

80.916

0.412
  ssb Latilactobacillus sakei subsp. sakei 23K

46.903

86.26

0.405

Multiple sequence alignment