Detailed information NTG1165825

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB3226_RS26540
• Genome accession: NZ_OZ061318
• Coordinates: 5804400..5805509 (+)
• Length: 369 a.a.
• NCBI ID: WP_367375278.1
• Uniprot ID: -
• Organism: Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5799400..5810509

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3226_RS26525	-	5800081..5801013 (+)	933	WP_367375276.1	sugar kinase	-
AB3226_RS26530	-	5801401..5801676 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
AB3226_RS26535	-	5801743..5804280 (-)	2538	WP_367375277.1	PAS domain-containing protein	-
AB3226_RS26540	pilU	5804400..5805509 (+)	1110	WP_367375278.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB3226_RS26545	-	5805678..5807633 (-)	1956	WP_367375279.1	acetoacetate--CoA ligase	-
AB3226_RS26550	hbdH	5807772..5808545 (-)	774	WP_367375280.1	3-hydroxybutyrate dehydrogenase	-
AB3226_RS26555	-	5808553..5809944 (-)	1392	WP_367375281.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40908.83 Da
• Isoelectric Point: 6.5716

>NTDB_id=1165825 AB3226_RS26540 WP_367375278.1 5804400..5805509(+) (pilU) [Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854]
MEIDALLQILASRNGSDLYLSTGAPPSAKFDGVLKALAEQPFKPGEIAAIAASIMDAEQRLEFDRELEMNLALSLAGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLILFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRTQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEIMLGTPTIGDLIRRNEFSELKGIMEKSSEAGMQTFDGAL
FELFAEGAIDEAEALKHADSVNNLRLRLKMHAEATPGPHTPPGEWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=1165825 AB3226_RS26540 WP_367375278.1 5804400..5805509(+) (pilU) [Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854]
ATGGAAATCGATGCACTGTTGCAAATTCTGGCCAGCCGCAATGGCTCCGATCTTTACCTGTCCACCGGCGCGCCACCCAG
CGCGAAATTTGACGGTGTGCTCAAAGCCTTGGCCGAGCAACCATTCAAGCCCGGAGAAATCGCGGCCATCGCGGCGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGCGAACTGGAAATGAACCTGGCGCTTTCCCTGGCCGGGGTCGGGCGC
TTTCGGCTCAATATCTTCAAGCAGCGCAACGATGTGTCCATCGTGGCGCGCAACATCAAACTCGACATTCCACGCTTCGA
AGACCTCAAACTGCCGCCGGTACTGCTCGAAACCGTGATGCTCAAACAAGGGCTGATATTGTTCGTCGGTTCGACCGGCT
CGGGCAAGTCGACATCGCTGGCGGCACTGATCGATTACCGCAATCGCCACAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTTATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTCGGCGTAGACACCCGCAGTTTCCATGC
TGCGTTGAAAAACACCCTGCGTCAGGCGCCGGACGTGGTGCTGATCGGCGAAATTCGCGATCGCGAGACTATGGAGCACG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTAATTTCCACTTTGCATGCGCACAACGCCAATCAGGCGCTGGACCGGGTG
ATCAACTTCTTCCCCGAAGAACGCCGGACGCAACTGCTCCATGACTTGGGTAATAACCTCAAGGCCTTTGTTTCCCAGCG
TTTGGTGCGTACCCGCGATGGGCAGCGCCGGGCGGCGGTGGAGATCATGCTGGGAACGCCGACCATCGGCGACCTGATCC
GGCGCAACGAATTTTCCGAGCTCAAGGGGATCATGGAGAAGTCTTCTGAGGCGGGGATGCAGACGTTTGATGGGGCGCTG
TTCGAGCTGTTTGCCGAGGGCGCGATCGATGAGGCAGAGGCGTTGAAACATGCGGACTCGGTGAACAACTTGCGCCTGCG
GCTGAAAATGCACGCTGAGGCTACGCCCGGGCCCCATACACCGCCGGGTGAGTGGGGATTAGTGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.201	100	0.542
pilU	Acinetobacter baylyi ADP1	53.09	96.477	0.512
pilU	Vibrio cholerae strain A1552	50.286	94.851	0.477
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	90.244	0.377
pilT	Vibrio cholerae strain A1552	41.742	90.244	0.377
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Pseudomonas aeruginosa PAK	40.116	93.225	0.374
pilT	Legionella pneumophila strain Lp02	40.719	90.515	0.369
pilT	Legionella pneumophila strain ERS1305867	40.719	90.515	0.369
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.118	91.87	0.369