Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AB3226_RS10175 Genome accession   NZ_OZ061318
Coordinates   2273318..2274535 (+) Length   405 a.a.
NCBI ID   WP_367372982.1    Uniprot ID   -
Organism   Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2268318..2279535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3226_RS10155 - 2269640..2269936 (+) 297 WP_367372980.1 DUF2845 domain-containing protein -
  AB3226_RS10160 - 2270293..2270649 (-) 357 WP_007901973.1 BON domain-containing protein -
  AB3226_RS10165 - 2270964..2271389 (-) 426 WP_218398775.1 pilin -
  AB3226_RS10170 pilB 2271615..2273315 (+) 1701 WP_367372981.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3226_RS10175 pilC 2273318..2274535 (+) 1218 WP_367372982.1 type II secretion system F family protein Machinery gene
  AB3226_RS10180 pilD 2274537..2275409 (+) 873 WP_367372983.1 A24 family peptidase Machinery gene
  AB3226_RS10185 coaE 2275406..2276029 (+) 624 WP_367372984.1 dephospho-CoA kinase -
  AB3226_RS10190 yacG 2276026..2276226 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  AB3226_RS10195 - 2276236..2276451 (-) 216 WP_007938959.1 hypothetical protein -
  AB3226_RS10200 - 2276515..2277204 (-) 690 WP_367372985.1 energy-coupling factor ABC transporter permease -
  AB3226_RS10205 ppk2 2277545..2278369 (+) 825 WP_367372986.1 polyphosphate kinase 2 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43976.91 Da        Isoelectric Point: 9.9824

>NTDB_id=1165804 AB3226_RS10175 WP_367372982.1 2273318..2274535(+) (pilC) [Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854]
MAVKAAKISVYAWEGTDKKGTKMTGELTGQNPALIKAQLRKQGISPGKVRKKTASIFSAGKRIKALDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFSGFGAELPAFTVMVIGLSEFLQAWWWMVLGVLVGAFFGVR
HALKKSQGLRDRMDTWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFAMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1165804 AB3226_RS10175 WP_367372982.1 2273318..2274535(+) (pilC) [Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854]
ATGGCGGTCAAAGCAGCGAAAATCAGCGTATATGCCTGGGAAGGCACAGACAAAAAAGGCACAAAAATGACCGGCGAGTT
GACCGGCCAGAATCCCGCGCTGATCAAGGCGCAGTTACGCAAGCAAGGCATCAGCCCGGGCAAGGTGCGGAAAAAAACCG
CATCCATTTTCAGTGCCGGCAAACGCATCAAGGCCCTGGATATTGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGTTGCTGCAATCGTTCGACATTATCGGCGAGGGCTTCGATAACCCGAACATGCGCAAGCTGGTGGACGA
GGTTAAACAGGAAGTCGCGGCCGGTAACAGCTTCGCCGCGGCCCTGCGCAAAAAACCCCAGTATTTCGATGAGCTGTACT
GCAACCTTGTTGATGCCGGCGAACAGGCCGGCGCTCTGGATACGCTGCTGGAACGGGTGGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACGTATCCGCTGGCCGTGGTGTTCGTCGCGATCATCGTGACCGGGAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCGAGTCGGTGTTCTCGGGGTTTGGCGCCGAACTACCGGCGTTCACCGTGATGG
TCATCGGCTTGTCGGAGTTCCTGCAGGCCTGGTGGTGGATGGTGCTCGGCGTGCTGGTTGGAGCATTCTTCGGCGTACGT
CACGCCTTGAAAAAGTCCCAGGGTCTGCGGGACCGAATGGACACCTGGCTGCTGAAACTGCCGTTGGTTGGCGCGCTCAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCTTGTCGACCACATTTGCCGCCGGGGTGCCATTGGTCGAAGCTCTGG
ATTCAGTGGCGGGCGCGACGGGCAATATCGTGTTCAAGCGAGCGGTGTTACGTATCAAGCAGGACGTATCCACCGGCATG
CAGCTGAATTTTGCCATGCGCACCTCCGGGATCTTTCCGAACATGGCGATCCAGATGACGGCCATCGGCGAAGAGTCCGG
CGCACTGGACGATATGCTCGACAAGGTCGCGAGTTTCTACGAGGCGGAGGTGGACAACATGGTCGATAACCTGACCAGTC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTTGTCGTCGGCGGCCTGGTCGTTGCGATGTACCTGCCCATCTTCCAA
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

43.672

99.506

0.435
  pilG Neisseria meningitidis 44/76-A

43.176

99.506

0.43
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

41.133

100

0.412
  pilC Thermus thermophilus HB27

38.806

99.259

0.385

Multiple sequence alignment