Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB3226_RS10170 Genome accession   NZ_OZ061318
Coordinates   2271615..2273315 (+) Length   566 a.a.
NCBI ID   WP_367372981.1    Uniprot ID   -
Organism   Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2266615..2278315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3226_RS10150 - 2266653..2269640 (+) 2988 WP_367372979.1 DUF748 domain-containing protein -
  AB3226_RS10155 - 2269640..2269936 (+) 297 WP_367372980.1 DUF2845 domain-containing protein -
  AB3226_RS10160 - 2270293..2270649 (-) 357 WP_007901973.1 BON domain-containing protein -
  AB3226_RS10165 - 2270964..2271389 (-) 426 WP_218398775.1 pilin -
  AB3226_RS10170 pilB 2271615..2273315 (+) 1701 WP_367372981.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3226_RS10175 pilC 2273318..2274535 (+) 1218 WP_367372982.1 type II secretion system F family protein Machinery gene
  AB3226_RS10180 pilD 2274537..2275409 (+) 873 WP_367372983.1 A24 family peptidase Machinery gene
  AB3226_RS10185 coaE 2275406..2276029 (+) 624 WP_367372984.1 dephospho-CoA kinase -
  AB3226_RS10190 yacG 2276026..2276226 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  AB3226_RS10195 - 2276236..2276451 (-) 216 WP_007938959.1 hypothetical protein -
  AB3226_RS10200 - 2276515..2277204 (-) 690 WP_367372985.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 63038.10 Da        Isoelectric Point: 5.6949

>NTDB_id=1165803 AB3226_RS10170 WP_367372981.1 2271615..2273315(+) (pilB) [Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRNRISLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLDKDTQPRGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEEDKLSDAIEKFFDNHSTGLEDMADVDLDG
LDIESVDEQKQDPIAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKIYRVRMRTDGMLREVARPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMHLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLRNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIDIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNNGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1165803 AB3226_RS10170 WP_367372981.1 2271615..2273315(+) (pilB) [Pseudomonas lini isolate Pseudomonas lini CIRM-BIA2854]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTACTGGCCGAGCTGATCACTGACCAAAGTGCGCAACAGGC
GTATCAGCAAGCCCAACGCAATCGAATTTCGCTGGTCAGTTATCTGGTGCAAAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCCTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAATTGCCTGGACAAGGACACCCAACCCCGAGGGCTG
GTCAGTGAGAAGCTGATCCGTCAGCACCACGCCCTGCCCCTCTGGCGACGTGGTAACAAGCTGTTCGTGGGGATTTCAGA
CCCGACCAATCATCAAGCCATCAACGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATCCTGGTGGAGGAAGACA
AACTCAGCGACGCCATCGAAAAATTCTTTGACAACCACTCCACCGGCCTGGAGGACATGGCCGATGTCGACCTCGACGGG
CTGGACATCGAGTCGGTCGATGAGCAGAAACAGGACCCCATTGCAGGGCAAGATACCGATGACGCGCCCGTGGTTCGCTT
CGTCCACAAGATGCTACTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTATGAAAAAATCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGGGAGGTCGCCAGGCCGCCGACGCAACTGGCCAACCGCATTGCTTCCCGACTG
AAGGTCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGAATCAAGATGCATCTGTCCAAAAGCAA
ATCCATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAGGTGGTAATCCGGATCCTCGACCCTTCCAGTG
CACAAATGGGCATCGACGCCCTCGGCTACGAACCCGAGCAAAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGA
ATGATCCTGGTAACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTCTATACCGGGTTGAATATCCTCAACACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTAGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAGGCAAG
GACTGGATTTCGCCCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAAATCCGCGATCTC
GAAACCGCCGAAATCGCCATCAAGGCTGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCTTGCGCAACATGGGGATTCAGGGATTCAACATCGCGACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGTCATTGCAAAAAGCCCATCGACATTCCCCGCGAGGCGTTACTCAAAGAAGGCTTTCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGATCAATGCAACAACGGTTACAAAGGGCGAGTAGGGAT
TTATGAAGTGGTAAAGAACACCCCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACATCCAGA
TGCGTAAGGACGGCTTCAACGACCTGCGTACTTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.865

99.647

0.587
  pilB Acinetobacter baylyi ADP1

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.929

99.823

0.558
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.273

97.173

0.479
  pilF Thermus thermophilus HB27

36.46

99.823

0.364

Multiple sequence alignment