Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   U9J37_RS13855 Genome accession   NZ_OY808997
Coordinates   2974413..2974814 (-) Length   133 a.a.
NCBI ID   WP_005475654.1    Uniprot ID   -
Organism   Vibrio sp. 16 isolate VDT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2969413..2979814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9J37_RS13835 (VDT1_2818) coaE 2969951..2970559 (-) 609 WP_322413840.1 dephospho-CoA kinase -
  U9J37_RS13840 (VDT1_2819) pilD 2970561..2971430 (-) 870 WP_039477926.1 prepilin peptidase Machinery gene
  U9J37_RS13845 (VDT1_2820) pilC 2971486..2972712 (-) 1227 WP_005475684.1 type II secretion system F family protein Machinery gene
  U9J37_RS13850 (VDT1_2821) pilB 2972725..2974413 (-) 1689 WP_043887354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U9J37_RS13855 (VDT1_2822) pilA 2974413..2974814 (-) 402 WP_005475654.1 type IV pilin protein Machinery gene
  U9J37_RS13860 (VDT1_2823) nadC 2975071..2975958 (-) 888 WP_005475682.1 carboxylating nicotinate-nucleotide diphosphorylase -
  U9J37_RS13865 (VDT1_2824) ampD 2976100..2976654 (+) 555 WP_005475679.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  U9J37_RS13870 (VDT1_2825) fldB 2976708..2977229 (-) 522 WP_005475668.1 flavodoxin FldB -
  U9J37_RS13875 (VDT1_2826) xerD 2977393..2978289 (+) 897 WP_005475659.1 site-specific tyrosine recombinase XerD -
  U9J37_RS13880 (VDT1_2827) - 2978318..2979061 (+) 744 WP_043887353.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 13214.12 Da        Isoelectric Point: 7.7791

>NTDB_id=1162000 U9J37_RS13855 WP_005475654.1 2974413..2974814(-) (pilA) [Vibrio sp. 16 isolate VDT1]
MKQTNNRKAKGFTLIELMIVVAVIGVLAAIAIPQYQKYVEKGALGSALATATALKTNYEDYLAVSGAAPASVSDIGAVSF
ALGTVSLDASGAIEVGITSGGGSGSSVKLARSVDGSWDCTISASAGVKINGCG

Nucleotide


Download         Length: 402 bp        

>NTDB_id=1162000 U9J37_RS13855 WP_005475654.1 2974413..2974814(-) (pilA) [Vibrio sp. 16 isolate VDT1]
ATGAAACAAACGAATAACCGTAAAGCAAAGGGTTTTACGCTGATTGAGTTGATGATAGTAGTGGCAGTTATCGGCGTATT
GGCGGCGATTGCCATCCCTCAGTACCAAAAATATGTGGAGAAAGGCGCATTAGGCTCAGCTCTAGCAACAGCGACCGCAC
TAAAAACAAACTATGAAGATTATTTAGCTGTAAGTGGCGCTGCTCCAGCGTCAGTTTCAGACATCGGCGCAGTTTCGTTT
GCACTAGGCACAGTATCTCTTGACGCTTCGGGAGCCATTGAAGTAGGTATAACATCAGGTGGTGGCTCTGGTAGTTCAGT
TAAACTAGCCAGATCGGTTGACGGTTCTTGGGACTGTACAATCAGTGCATCCGCAGGTGTCAAAATTAATGGATGCGGCT
AG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(45-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.26

100

0.519
  pilA Vibrio cholerae strain A1552

47.26

100

0.519
  pilA Vibrio cholerae C6706

47.26

100

0.519
  pilA Pseudomonas aeruginosa PAK

40.278

100

0.436
  pilA Acinetobacter baumannii strain A118

43.609

100

0.436
  pilA Vibrio parahaemolyticus RIMD 2210633

38.514

100

0.429
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

100

0.406
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.394

99.248

0.391

Multiple sequence alignment