Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLMBN_RS13325 Genome accession   NZ_OY760626
Coordinates   2827480..2828742 (+) Length   420 a.a.
NCBI ID   WP_321407222.1    Uniprot ID   -
Organism   MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2822480..2833742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBN_RS13310 (U2939_RS13260) purD 2823642..2824928 (+) 1287 WP_321407215.1 phosphoribosylamine--glycine ligase -
  ACLMBN_RS13315 (U2939_RS13265) - 2825225..2825680 (+) 456 WP_321407217.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACLMBN_RS13320 (U2939_RS13270) pilB 2825803..2827461 (+) 1659 WP_411354443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLMBN_RS13325 (U2939_RS13275) pilC 2827480..2828742 (+) 1263 WP_321407222.1 type II secretion system F family protein Machinery gene
  ACLMBN_RS13330 (U2939_RS13280) pilD 2828757..2829632 (+) 876 WP_321407223.1 A24 family peptidase Machinery gene
  ACLMBN_RS13335 (U2939_RS13285) coaE 2829632..2830249 (+) 618 WP_321407225.1 dephospho-CoA kinase -
  ACLMBN_RS13340 (U2939_RS13290) yacG 2830298..2830516 (+) 219 WP_012728762.1 DNA gyrase inhibitor YacG -
  ACLMBN_RS13345 (U2939_RS13295) mutT 2830513..2830911 (-) 399 WP_321407229.1 8-oxo-dGTP diphosphatase MutT -
  ACLMBN_RS13350 (U2939_RS13300) secA 2830980..2833703 (-) 2724 WP_321407231.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 46226.56 Da        Isoelectric Point: 9.8705

>NTDB_id=1161521 ACLMBN_RS13325 WP_321407222.1 2827480..2828742(+) (pilC) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
MAVAQQSSAAATANKPQPRVSQSFVWRGVNRRGQKVSGEMKGETLAQVKADLIRQGVVVQKISKHQESIVTRYTQTIKAM
DIAVLTRQIATMLTAGVPLVQTLRLLASSHEKRPMREMLAAICADIETGTPPSKALRQFPRYFDDLYCDLVASGEQSGAL
DAVFDRIAIYREKAEALKSKIKKAMFYPATVIFVALVVTAILLIFVIPEFDKIFKSFGAELPAFTRMIITLSRVVQTYWY
FVFGGLVALIFVYVHSYRRMESVRDATDKFILKLPIVGLILQKAALARYARTLSTTFAAGLPLIDGLIAAAGSSGNAVYR
HAIMNVRNEVMSGMQMNVAMRTTNVFPEMVIQMIMIGEESGSLDDMLSKVAGIYEQQVDDAVDALSSLIEPLLMVVLGIL
VGGMVIAMYLPIFKLGSVIH

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=1161521 ACLMBN_RS13325 WP_321407222.1 2827480..2828742(+) (pilC) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
ATGGCCGTGGCTCAACAATCCTCCGCCGCTGCAACGGCAAATAAACCGCAACCCAGAGTGTCACAGTCATTTGTCTGGCG
CGGTGTTAACCGCCGCGGTCAGAAAGTGTCCGGTGAAATGAAAGGTGAGACGCTGGCGCAGGTCAAGGCTGATCTGATCC
GTCAGGGGGTTGTGGTGCAGAAAATAAGCAAACACCAGGAATCCATTGTCACCCGCTACACGCAAACCATCAAAGCAATG
GATATTGCGGTATTAACCCGCCAGATTGCCACCATGCTGACTGCCGGTGTACCACTGGTGCAGACACTGCGCCTGCTCGC
CAGCAGCCATGAAAAGCGCCCGATGCGGGAAATGCTGGCCGCGATCTGCGCTGACATTGAAACCGGTACGCCGCCATCCA
AAGCCTTACGTCAGTTCCCGCGTTATTTTGATGATCTCTATTGTGATCTGGTGGCCTCCGGTGAACAGTCCGGCGCGCTG
GATGCGGTATTTGATCGGATTGCCATCTACCGGGAAAAAGCCGAGGCGCTGAAATCAAAAATCAAAAAAGCCATGTTTTA
TCCGGCAACCGTTATCTTTGTCGCCTTGGTGGTTACCGCGATTCTTTTGATCTTTGTAATCCCTGAATTTGATAAAATTT
TCAAAAGCTTCGGTGCTGAACTCCCCGCCTTTACCCGCATGATTATTACACTGTCACGTGTAGTGCAGACCTACTGGTAC
TTTGTTTTTGGTGGTCTGGTCGCCCTGATTTTTGTTTATGTTCACTCTTACCGGCGCATGGAATCCGTCCGTGATGCTAC
CGATAAGTTCATCCTGAAACTGCCGATTGTGGGTCTCATCCTGCAAAAAGCCGCGCTGGCCCGTTACGCCCGTACCCTTT
CCACCACCTTTGCTGCGGGTCTGCCGCTGATTGACGGCTTGATCGCCGCTGCAGGTTCCTCCGGCAATGCGGTATACCGT
CACGCCATCATGAATGTCCGTAACGAAGTAATGTCAGGCATGCAGATGAACGTCGCCATGCGCACCACCAACGTGTTTCC
GGAAATGGTCATCCAGATGATCATGATCGGTGAAGAATCAGGCTCGCTGGATGATATGCTTTCCAAAGTCGCCGGGATCT
ATGAGCAACAGGTAGATGATGCCGTCGATGCGCTCTCCAGCCTGATTGAACCGTTACTCATGGTGGTATTGGGTATTCTG
GTCGGTGGTATGGTTATCGCCATGTATCTGCCAATCTTTAAACTTGGCAGCGTTATTCATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(290-411)

Type II secretion system (T2SS), protein F (T2SSF)

(86-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

51.768

94.286

0.488
  pilC Acinetobacter baumannii D1279779

50.503

94.762

0.479
  pilC Acinetobacter baylyi ADP1

49.622

94.524

0.469
  pilC Legionella pneumophila strain ERS1305867

49.37

94.524

0.467
  pilC Vibrio cholerae strain A1552

48.148

96.429

0.464
  pilC Vibrio campbellii strain DS40M4

46.734

94.762

0.443
  pilG Neisseria meningitidis 44/76-A

39.599

95

0.376
  pilG Neisseria gonorrhoeae MS11

39.599

95

0.376

Multiple sequence alignment