Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLMBN_RS13320 Genome accession   NZ_OY760626
Coordinates   2825803..2827461 (+) Length   552 a.a.
NCBI ID   WP_411354443.1    Uniprot ID   -
Organism   MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2820803..2832461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBN_RS13305 (U2939_RS13255) purH 2821685..2823277 (+) 1593 WP_321407212.1 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase -
  ACLMBN_RS13310 (U2939_RS13260) purD 2823642..2824928 (+) 1287 WP_321407215.1 phosphoribosylamine--glycine ligase -
  ACLMBN_RS13315 (U2939_RS13265) - 2825225..2825680 (+) 456 WP_321407217.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACLMBN_RS13320 (U2939_RS13270) pilB 2825803..2827461 (+) 1659 WP_411354443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLMBN_RS13325 (U2939_RS13275) pilC 2827480..2828742 (+) 1263 WP_321407222.1 type II secretion system F family protein Machinery gene
  ACLMBN_RS13330 (U2939_RS13280) pilD 2828757..2829632 (+) 876 WP_321407223.1 A24 family peptidase Machinery gene
  ACLMBN_RS13335 (U2939_RS13285) coaE 2829632..2830249 (+) 618 WP_321407225.1 dephospho-CoA kinase -
  ACLMBN_RS13340 (U2939_RS13290) yacG 2830298..2830516 (+) 219 WP_012728762.1 DNA gyrase inhibitor YacG -
  ACLMBN_RS13345 (U2939_RS13295) mutT 2830513..2830911 (-) 399 WP_321407229.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 61269.12 Da        Isoelectric Point: 5.1193

>NTDB_id=1161520 ACLMBN_RS13320 WP_411354443.1 2825803..2827461(+) (pilB) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
MIQHDQSVSLQEKARNEKKTLVTYLIDNNIISSSQLAELCELEYGIPLLDLDAFELSEIPHKYLNAKLIEKHHALPIYTQ
GNTLYIALSDPTNITALEDFGFNFNLHTEALLVDERKLQNAISRILEPTEDDFSKSMSDDHIGDIEVSDMDEARLQESLE
TNSDDDAPIVKYINKVLLDAVRRGASDLHFEPYEKKYRIRFRIDGILYEIATPPVNLANRFSARLKVMSRLDIAERRLPQ
DGRIKLRLAKNRSVDLRVSTLPTMWGEKVVLRILDSSAASLNIDMLGFDDKQKQLYLDTLARPQGMILVTGPTGSGKTVS
LYTGLNILNTSELNISTAEDPIEINLAGINQVQVNPKAGLTFANALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHL
VLSTLHTNSAAETLTRLLNMGVPAFNVASSVTLIIAQRLARRLCPHCKKPHQIPEHELLGMGYTPDEVTAGITLYKPVGC
EECSNGYKGRVGIYEMLPMSENIANLIMEGGNSLQIAETAVNEGMVTLYRSGLEKARAGVTSLAEVNRVTCI

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=1161520 ACLMBN_RS13320 WP_411354443.1 2825803..2827461(+) (pilB) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
ATCATTCAGCATGATCAGTCCGTGTCGTTACAGGAAAAAGCCCGTAACGAAAAAAAAACGCTGGTCACTTATCTGATTGA
TAACAATATTATCAGCAGCAGCCAGTTGGCGGAGCTCTGTGAGCTGGAATATGGTATCCCGCTGCTGGATCTGGATGCCT
TTGAACTCAGCGAGATCCCGCACAAATATCTCAATGCCAAACTAATCGAAAAACACCACGCCTTACCGATTTATACCCAG
GGTAACACCCTCTACATCGCACTATCTGACCCGACCAATATCACCGCGTTGGAAGATTTTGGCTTCAACTTCAACCTGCA
CACCGAAGCCCTGCTGGTGGATGAGCGTAAACTGCAAAATGCGATATCCCGCATTCTGGAACCCACCGAGGATGACTTTT
CCAAATCGATGTCGGATGACCATATCGGTGATATTGAAGTTAGCGATATGGATGAGGCCAGGCTGCAGGAATCACTTGAA
ACTAACAGTGACGATGATGCGCCGATTGTTAAATATATCAACAAGGTGCTGCTCGATGCGGTGCGCCGCGGCGCTTCTGA
CTTGCATTTCGAGCCGTATGAGAAAAAATACCGTATCCGCTTTCGTATCGACGGTATTTTATATGAAATCGCCACCCCGC
CGGTCAATCTGGCCAACCGTTTCTCGGCCCGCCTCAAGGTGATGTCACGTCTGGATATCGCCGAGCGCCGCTTACCGCAG
GATGGCCGTATAAAACTACGTCTGGCAAAAAACCGCTCCGTTGATCTGCGTGTCAGCACCCTGCCCACTATGTGGGGCGA
AAAAGTGGTACTGCGTATCCTCGATTCTTCTGCCGCCAGCCTGAACATCGATATGCTCGGCTTCGATGACAAGCAGAAAC
AGCTTTATCTTGATACCCTTGCCCGGCCGCAGGGTATGATCCTGGTCACCGGCCCGACCGGTTCCGGTAAAACCGTATCG
CTCTATACCGGCCTGAATATTCTTAATACCAGTGAGCTGAATATTTCCACCGCGGAAGACCCGATAGAGATCAACCTGGC
CGGGATTAATCAGGTGCAGGTCAACCCCAAAGCCGGCCTCACCTTTGCCAACGCCCTGCGCTCTTTCCTGCGTCAGGATC
CGGATGTCGTGATGGTCGGCGAAATCCGTGACCTTGAAACCGCGGAAATCGCCATTAAAGCGGCACAAACCGGCCACTTG
GTGTTATCCACCCTGCACACCAATTCCGCCGCCGAAACCCTGACCCGCTTGCTGAACATGGGCGTGCCGGCCTTTAACGT
CGCCTCATCGGTAACCCTGATCATCGCACAGCGGCTGGCCCGTCGCCTCTGTCCGCACTGTAAAAAGCCGCATCAGATCC
CGGAGCATGAGCTGTTGGGGATGGGCTATACTCCGGATGAAGTCACCGCCGGCATCACACTCTACAAACCAGTGGGTTGT
GAGGAATGTTCCAACGGCTATAAAGGCCGCGTCGGCATTTATGAAATGCTGCCGATGTCTGAAAACATCGCCAATCTGAT
CATGGAAGGCGGTAACTCATTACAAATCGCCGAAACCGCAGTCAATGAAGGCATGGTGACGCTCTACCGTTCCGGTCTGG
AGAAAGCACGGGCCGGCGTCACCAGTCTGGCTGAAGTCAACCGCGTGACCTGCATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-127)

Type II/IV secretion system protein (T2SSE)

(175-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.577

100

0.569
  pilB Acinetobacter baylyi ADP1

57.486

98.007

0.563
  pilB Vibrio cholerae strain A1552

57.116

96.739

0.553
  pilB Legionella pneumophila strain ERS1305867

54.25

100

0.543
  pilB Vibrio parahaemolyticus RIMD 2210633

53.226

100

0.538
  pilB Vibrio campbellii strain DS40M4

52.338

100

0.527
  pilF Neisseria gonorrhoeae MS11

48.776

96.196

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

39.858

100

0.406
  pilF Thermus thermophilus HB27

38.321

99.275

0.38

Multiple sequence alignment