Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N018_RS21275 Genome accession   NZ_CP007014
Coordinates   4804767..4805984 (+) Length   405 a.a.
NCBI ID   WP_025390694.1    Uniprot ID   W0MW84
Organism   Pseudomonas syringae CC1557     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4799767..4810984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N018_RS21245 (N018_21700) - 4799787..4800194 (-) 408 WP_025390688.1 hypothetical protein -
  N018_RS21250 (N018_21705) - 4800191..4800883 (-) 693 WP_038401509.1 DUF433 domain-containing protein -
  N018_RS21255 (N018_21710) gstA 4801121..4801741 (-) 621 WP_025390690.1 glutathione transferase GstA -
  N018_RS21260 (N018_21715) - 4801897..4802256 (+) 360 WP_025390691.1 winged helix-turn-helix transcriptional regulator -
  N018_RS26845 - 4802440..4802844 (-) 405 WP_025390692.1 pilin -
  N018_RS21270 (N018_21720) pilB 4803070..4804764 (+) 1695 WP_025390693.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N018_RS21275 (N018_21725) pilC 4804767..4805984 (+) 1218 WP_025390694.1 type II secretion system F family protein Machinery gene
  N018_RS21280 (N018_21730) pilD 4805986..4806858 (+) 873 WP_025390695.1 prepilin peptidase Machinery gene
  N018_RS21285 (N018_21735) coaE 4806855..4807478 (+) 624 WP_025390696.1 dephospho-CoA kinase -
  N018_RS21290 (N018_21740) yacG 4807475..4807684 (+) 210 WP_025390697.1 DNA gyrase inhibitor YacG -
  N018_RS21295 (N018_21745) - 4807936..4808262 (-) 327 WP_025390698.1 hypothetical protein -
  N018_RS21300 - 4808458..4809085 (+) 628 Protein_4252 DUF1780 domain-containing protein -
  N018_RS21305 (N018_21760) - 4809082..4809615 (+) 534 WP_024645222.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44122.19 Da        Isoelectric Point: 10.1956

>NTDB_id=115729 N018_RS21275 WP_025390694.1 4804767..4805984(+) (pilC) [Pseudomonas syringae CC1557]
MASKAAKVIVYTWEGVDKKGTKTSGELSGHNLALIKAQLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFARQMATMMK
AGVPLLQSFDIIGEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAIIVSGILLIKVVPQFQTVFASFGAQLPAFTLMVIGLSDIVQKWWLAIVALFFVSFFMFK
RAYKQSQKFRDSLDRLLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVIKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=115729 N018_RS21275 WP_025390694.1 4804767..4805984(+) (pilC) [Pseudomonas syringae CC1557]
ATGGCCAGCAAGGCAGCAAAAGTCATCGTTTACACGTGGGAAGGTGTCGACAAGAAAGGTACTAAAACCAGTGGGGAGCT
GAGTGGCCATAACTTGGCTCTTATAAAGGCCCAGCTGCGCAAGCAAGGCATCAACCCAACCAAGGTACGCAAAAAATCTG
TTTCGATCTTCGGCAAGGGAAAAAAAATCAAGCCGCTGGACATCGCGTTTTTCGCTCGACAGATGGCAACAATGATGAAG
GCTGGCGTGCCTCTGCTCCAATCATTCGACATCATTGGTGAAGGCGCCGAGAACCCAAATATGCGTGCATTGGTAGGCTC
GCTAAAGCAGGAAGTTTCGGCAGGTAATAGTTTTGCGACGGCTCTGCGACAAAAACCGGAATATTTCGATGACCTGTTTT
GTAATCTGGTAGATGCTGGCGAGCAAGCTGGCGCACTTGAAAGCCTGCTGGATCGCGTTGCTAGTTACAAGGAAAAAACT
GAAAAGCTAAAAGCTAAAATCAAAAAAGCCATGACATACCCTATTGCTGTTTTGATCGTAGCAATTATTGTGTCAGGAAT
TTTGCTCATCAAGGTAGTGCCACAGTTCCAGACGGTCTTCGCCAGCTTCGGCGCTCAGCTACCTGCATTTACTTTGATGG
TGATTGGCCTATCGGACATTGTACAAAAATGGTGGTTGGCCATCGTCGCACTATTTTTCGTTAGCTTTTTTATGTTCAAA
CGCGCGTACAAACAGTCTCAAAAGTTTCGAGACAGCCTCGACCGACTTCTGCTGAAAGTGCCCATCATCGGGCCATTAAT
TTTCAAATCTTCGGTGGCACGCTACGCTCGCACGCTTGCAACAACCTTTGCAGCTGGCGTCCCGCTTGTAGAGGCTTTGG
ACTCGGTCGCCGGTGCGACTGGCAATGTGGTATTCAAAAATGCGGTTATCAAGGTCAAGCAGGACGTCTCCACAGGCATG
CAATTGAATTTCTCGATGCGTTCAACAGGGGTCTTTCCGAGCTTGGCGATTCAGATGACCGCTATTGGCGAAGAGTCCGG
TGCCTTGGATACCATGCTGGACAAGGTCGCTACCTACTACGAGGACGAAGTCGATAACATGGTCGACAATCTGACCAGCC
TGATGGAACCGATGATCATGGCCTTTTTGGGCGTGATCGTCGGCGGGCTGGTCATTGCGATGTATCTACCTATCTTCCAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0MW84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

60.294

100

0.607
  pilC Acinetobacter baumannii D1279779

60.049

100

0.605
  pilC Legionella pneumophila strain ERS1305867

50.246

100

0.504
  pilG Neisseria gonorrhoeae MS11

43.325

98.025

0.425
  pilG Neisseria meningitidis 44/76-A

43.325

98.025

0.425
  pilC Vibrio campbellii strain DS40M4

42.015

100

0.422
  pilC Vibrio cholerae strain A1552

42.172

97.778

0.412
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment