Detailed information NTG0115730

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N018_RS21280
• Genome accession: NZ_CP007014
• Coordinates: 4805986..4806858 (+)
• Length: 290 a.a.
• NCBI ID: WP_025390695.1
• Uniprot ID: W0N092
• Organism: Pseudomonas syringae CC1557
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4800986..4811858

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N018_RS21255 (N018_21710)	gstA	4801121..4801741 (-)	621	WP_025390690.1	glutathione transferase GstA	-
N018_RS21260 (N018_21715)	-	4801897..4802256 (+)	360	WP_025390691.1	winged helix-turn-helix transcriptional regulator	-
N018_RS26845	-	4802440..4802844 (-)	405	WP_025390692.1	pilin	-
N018_RS21270 (N018_21720)	pilB	4803070..4804764 (+)	1695	WP_025390693.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N018_RS21275 (N018_21725)	pilC	4804767..4805984 (+)	1218	WP_025390694.1	type II secretion system F family protein	Machinery gene
N018_RS21280 (N018_21730)	pilD	4805986..4806858 (+)	873	WP_025390695.1	prepilin peptidase	Machinery gene
N018_RS21285 (N018_21735)	coaE	4806855..4807478 (+)	624	WP_025390696.1	dephospho-CoA kinase	-
N018_RS21290 (N018_21740)	yacG	4807475..4807684 (+)	210	WP_025390697.1	DNA gyrase inhibitor YacG	-
N018_RS21295 (N018_21745)	-	4807936..4808262 (-)	327	WP_025390698.1	hypothetical protein	-
N018_RS21300	-	4808458..4809085 (+)	628	Protein_4252	DUF1780 domain-containing protein	-
N018_RS21305 (N018_21760)	-	4809082..4809615 (+)	534	WP_024645222.1	MOSC domain-containing protein	-
N018_RS21310 (N018_21765)	-	4809832..4811130 (+)	1299	WP_025390699.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31980.11 Da
• Isoelectric Point: 7.6444

>NTDB_id=115730 N018_RS21280 WP_025390695.1 4805986..4806858(+) (pilD) [Pseudomonas syringae CC1557]
MPLLDLLASSPLAFVTTCCILGLIVGSFLNVVVYRLPIMMERDWKTQSREMLGLPAEPDQPVFNLTLPHSSCPHCAHKIR
PWENLPIISYLLLRGKCSHCQAPISKRYPLVELTCAVLSAYIAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNSLGLFTSLSDALWGAVAGYLTLWSVYWLFKLVTGKEGMGHGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=115730 N018_RS21280 WP_025390695.1 4805986..4806858(+) (pilD) [Pseudomonas syringae CC1557]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATACTAGGCCTCATCGTCGGAAG
CTTCCTCAACGTTGTGGTTTACCGCCTCCCAATAATGATGGAGCGCGACTGGAAAACCCAATCCCGTGAAATGCTCGGTC
TCCCCGCCGAACCGGACCAACCCGTCTTCAACCTGACCCTCCCCCACTCCAGTTGCCCGCACTGCGCGCACAAGATCCGC
CCGTGGGAAAACCTGCCGATCATCAGCTATCTGCTGTTGCGCGGGAAATGCTCGCACTGCCAAGCCCCCATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTACATCGCGTGGCACTTCGGCTTCGGCTGGCAAGCGGCGG
CAATGCTAGTGCTGAGCTGGGGCCTGTTGGCGATGAGTCTGATTGATGCTGATCACCAACTACTGCCTGACTCGCTGGTG
CTGCCGCTGCTATGGCTGGGCCTGATCGTTAACTCGCTCGGGCTGTTCACCTCGCTGAGCGATGCGCTGTGGGGCGCGGT
GGCGGGTTATCTGACGCTGTGGTCGGTGTATTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCCATGGCGATT
TCAAGCTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGCTGTCATCGCTGGTGGGC
GCGGTATTGGGAGTGATCATGATGCGTGTGCGCCGGGTTGAAAGCGGCACGCCGATCCCCTTTGGCCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: W0N092

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.417	97.586	0.531
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	51.46	94.483	0.486
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Neisseria gonorrhoeae MS11	52.239	92.414	0.483