Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N018_RS21270 Genome accession   NZ_CP007014
Coordinates   4803070..4804764 (+) Length   564 a.a.
NCBI ID   WP_025390693.1    Uniprot ID   -
Organism   Pseudomonas syringae CC1557     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4798070..4809764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N018_RS21235 (N018_21690) - 4798075..4799073 (-) 999 WP_025390686.1 ATP-grasp domain-containing protein -
  N018_RS21240 (N018_21695) - 4799194..4799739 (+) 546 WP_025390687.1 DUF1523 family protein -
  N018_RS21245 (N018_21700) - 4799787..4800194 (-) 408 WP_025390688.1 hypothetical protein -
  N018_RS21250 (N018_21705) - 4800191..4800883 (-) 693 WP_038401509.1 DUF433 domain-containing protein -
  N018_RS21255 (N018_21710) gstA 4801121..4801741 (-) 621 WP_025390690.1 glutathione transferase GstA -
  N018_RS21260 (N018_21715) - 4801897..4802256 (+) 360 WP_025390691.1 winged helix-turn-helix transcriptional regulator -
  N018_RS26845 - 4802440..4802844 (-) 405 WP_025390692.1 pilin -
  N018_RS21270 (N018_21720) pilB 4803070..4804764 (+) 1695 WP_025390693.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N018_RS21275 (N018_21725) pilC 4804767..4805984 (+) 1218 WP_025390694.1 type II secretion system F family protein Machinery gene
  N018_RS21280 (N018_21730) pilD 4805986..4806858 (+) 873 WP_025390695.1 prepilin peptidase Machinery gene
  N018_RS21285 (N018_21735) coaE 4806855..4807478 (+) 624 WP_025390696.1 dephospho-CoA kinase -
  N018_RS21290 (N018_21740) yacG 4807475..4807684 (+) 210 WP_025390697.1 DNA gyrase inhibitor YacG -
  N018_RS21295 (N018_21745) - 4807936..4808262 (-) 327 WP_025390698.1 hypothetical protein -
  N018_RS21300 - 4808458..4809085 (+) 628 Protein_4252 DUF1780 domain-containing protein -
  N018_RS21305 (N018_21760) - 4809082..4809615 (+) 534 WP_024645222.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62499.81 Da        Isoelectric Point: 6.3854

>NTDB_id=115728 N018_RS21270 WP_025390693.1 4803070..4804764(+) (pilB) [Pseudomonas syringae CC1557]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIKEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=115728 N018_RS21270 WP_025390693.1 4803070..4804764(+) (pilB) [Pseudomonas syringae CC1557]
ATGACTGATGCTGTCCTCACTGGCCTGGCCAAGCAATTGGTCCTCGCCGAGCTACTTACTGAACCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTCAGCTATCTGGTTCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGATCAGTTTGGTGTGCCGTTCATGGACCTCGCCAGTCTGGACAAGGAAAGCCAGCCCAAGGGGCTA
GTGAGCGAAAAGCTGGTTCGACAGCATCACGCTCTCCCACTCTGGCGACGCGGCAATAAGCTGTTCATCGCCATATCCGA
CCCTACCAACCATCAGGCAGTGACTGATATTCAGTTCAGCACCGGCCTGAATACCGAAGCTATTCTGGTCGAAGATGACA
AGCTGACTACGGCAATAGATCGCTTCTTTGACAGCGATAGCGGTCTGGGCAATCTGGAAGATGTTGATCTGGGTCTGGAT
ATTGAACCAGCTGATGGCAAGGAAACGTCTCTCGCTACGCAGAACGACGCAGACGATGCCCCTGTGGTGCGCTTCGTCAA
CAAGATGTTGATGGATGCTATCCGGCTAGGCTCTTCCGACCTGCATTTTGAGCCTTACGAGAAGATCTTCCGCGTGCGCT
TGCGTACCGACGGCATCCTGCATGAAGTCGCCAGACCGCCAATTCACCTGGCGAACCGAATTGCTGCACGCCTCAAGGTA
ATGGCCAGCCTCGACATCTCGGAACGACGTAAACCGCAAGACGGTCGGGTCAAGTTGCGGGTATCCAAAACAAAATCAAT
CGACTTTCGGATGAACACCCTGCCTACGCTATGGGGTGAGAAGATCGTGATGCGGATTCTCGACCCGACCAGCGCTCAGA
TGGGCATCGATGCTCTGGGCTACGAGCCAGAACAAAAAGCACTGTATATGGAGGCGCTGAAACAGCCGCAAGGCATGATT
CTTGTGACCGGCCCGACGGGTTCAGGCAAGACCGTTTCCCTGTATACCGGTCTGAATATTCTCAACACCGTAGACATCAA
TATTTCCACAGCTGAAGACCCGGTGGAGATCAATCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAAGGACTGG
ACTTTTCCCAAGCGTTGCGCGCGTTCCTTCGTCAAGATCCCGACGTTATCATGGTGGGTGAGATTCGAGACTTGGAAACA
GCCGAAATCGCCATCAAGGCGTCGCAGACCGGCCATATGGTGCTGTCCACCCTGCACACTAACAGCGCGGCAGAAACCTT
GACGCGACTGCATCATATGGGTGTCGCAGCATTCAACATTGCGACAGCAATCAACCTGATTATTGCGCAAAGACTTGCAC
GCAAATTATGCAGCCACTGCAAGAAAGAACTCGACATTCCTCGCGAGACACTGATTAAAGAAGGCTTCCCCGAAGCGAAG
ATCGGTACGTTCAAGATCTATGGCCCGGTAGGCTGCGAGCATTGCAACGGTGGCTACAGAGGCCGGGTCGGCATTTACGA
GGTGGTCAAGAAAACAGCGGAGCTGGAGCGCATCATCATGGAAGAGGGCAACTCGTTGGAGATTTCCAGGCAAATGCGCA
AGGATGGCTTCAATGACCTGCGTACCTCAGGCCTGTCAAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.582
  pilB Acinetobacter baylyi ADP1

56.89

100

0.571
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio cholerae strain A1552

50.534

99.645

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.268

99.113

0.498
  pilB Vibrio campbellii strain DS40M4

49.117

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.221

99.645

0.481
  pilF Thermus thermophilus HB27

38.869

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

93.262

0.363

Multiple sequence alignment