Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VPUCM_RS14370 Genome accession   NZ_CP007004
Coordinates   3092370..3094118 (-) Length   582 a.a.
NCBI ID   WP_025442303.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus UCM-V493     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3087370..3099118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPUCM_RS14350 (VPUCM_2845) - 3088052..3088891 (-) 840 WP_021484674.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VPUCM_RS14355 (VPUCM_2846) - 3088961..3090478 (-) 1518 WP_025442301.1 SPOR domain-containing protein -
  VPUCM_RS14360 (VPUCM_2847) aroB 3090531..3091631 (-) 1101 WP_025442302.1 3-dehydroquinate synthase -
  VPUCM_RS14365 (VPUCM_2848) aroK 3091663..3092181 (-) 519 WP_005381319.1 shikimate kinase AroK -
  VPUCM_RS14370 (VPUCM_2849) pilQ 3092370..3094118 (-) 1749 WP_025442303.1 type IV pilus secretin PilQ Machinery gene
  VPUCM_RS14375 (VPUCM_2850) pilP 3094150..3094665 (-) 516 WP_025442304.1 pilus assembly protein PilP Machinery gene
  VPUCM_RS14380 (VPUCM_2851) pilO 3094655..3095248 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  VPUCM_RS14385 (VPUCM_2852) pilN 3095241..3095819 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  VPUCM_RS14390 (VPUCM_2853) pilM 3095803..3096819 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63782.91 Da        Isoelectric Point: 4.4619

>NTDB_id=115470 VPUCM_RS14370 WP_025442303.1 3092370..3094118(-) (pilQ) [Vibrio parahaemolyticus UCM-V493]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGSDGEGEEGGDGVGIDDFLNVNLAATNKSA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=115470 VPUCM_RS14370 WP_025442303.1 3092370..3094118(-) (pilQ) [Vibrio parahaemolyticus UCM-V493]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAACAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGACTCTGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAGCAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCTCCGACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGGGAACTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATTAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTAAAA
CAAGTACAAATTGAAGCCCGAATTGTCACCATCAATGAGGGGAACATGGATGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTTGCGACAATCGGCTTGTACGAAGGTGGTAGTGACG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTCCTTAATGTGAACTTAGCTGCAACTAATAAAAGTGCT
GCAAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTACAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCTGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGGCCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGCACCCAAGTTCTGGTGAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTCCAGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGTAAAAGTGAGTTGCTAATTTTCGTTACACCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.409

100

0.914
  pilQ Vibrio cholerae strain A1552

76.408

97.595

0.746
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.408

97.595

0.746
  pilQ Pseudomonas aeruginosa PAK

38.879

100

0.393
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.502

0.39
  pilQ Legionella pneumophila strain Lp02

41.273

94.502

0.39
  pilQ Acinetobacter baumannii D1279779

37.458

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.458

100

0.385
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment