Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VPUCM_RS14390 Genome accession   NZ_CP007004
Coordinates   3095803..3096819 (-) Length   338 a.a.
NCBI ID   WP_005496568.1    Uniprot ID   A0A3E1IL27
Organism   Vibrio parahaemolyticus UCM-V493     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3090803..3101819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPUCM_RS14365 (VPUCM_2848) aroK 3091663..3092181 (-) 519 WP_005381319.1 shikimate kinase AroK -
  VPUCM_RS14370 (VPUCM_2849) pilQ 3092370..3094118 (-) 1749 WP_025442303.1 type IV pilus secretin PilQ Machinery gene
  VPUCM_RS14375 (VPUCM_2850) pilP 3094150..3094665 (-) 516 WP_025442304.1 pilus assembly protein PilP Machinery gene
  VPUCM_RS14380 (VPUCM_2851) pilO 3094655..3095248 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  VPUCM_RS14385 (VPUCM_2852) pilN 3095241..3095819 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  VPUCM_RS14390 (VPUCM_2853) pilM 3095803..3096819 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  VPUCM_RS14395 (VPUCM_2854) - 3096962..3099532 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  VPUCM_RS14400 (VPUCM_2855) oxyR 3099589..3100497 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  VPUCM_RS14405 (VPUCM_2856) - 3100668..3101396 (+) 729 WP_025442305.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=115474 VPUCM_RS14390 WP_005496568.1 3095803..3096819(-) (pilM) [Vibrio parahaemolyticus UCM-V493]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=115474 VPUCM_RS14390 WP_005496568.1 3095803..3096819(-) (pilM) [Vibrio parahaemolyticus UCM-V493]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1IL27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment