Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OC536_RS02525 Genome accession   NZ_OW443150
Coordinates   534096..535784 (-) Length   562 a.a.
NCBI ID   WP_032476649.1    Uniprot ID   -
Organism   Vibrio cholerae strain CNRVC190247 isolate YE-NCPHL-18035-PI     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 529096..540784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC536_RS02500 (CNRVC190247_00507) yacG 530162..530359 (-) 198 WP_032476646.1 DNA gyrase inhibitor YacG -
  OC536_RS02505 (CNRVC190247_00508) zapD 530517..531257 (-) 741 WP_000207194.1 cell division protein ZapD -
  OC536_RS02510 (CNRVC190247_00509) coaE 531289..531897 (-) 609 WP_000011550.1 dephospho-CoA kinase -
  OC536_RS02515 (CNRVC190247_00510) pilD 531894..532769 (-) 876 WP_032476647.1 A24 family peptidase Machinery gene
  OC536_RS02520 (CNRVC190247_00511) pilC 532827..534053 (-) 1227 WP_032476648.1 type II secretion system F family protein Machinery gene
  OC536_RS02525 (CNRVC190247_00512) pilB 534096..535784 (-) 1689 WP_032476649.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OC536_RS02530 (CNRVC190247_00513) - 535791..536201 (-) 411 WP_032476650.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OC536_RS02535 (CNRVC190247_00514) nadC 536447..537337 (-) 891 WP_000665277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OC536_RS02540 (CNRVC190247_00515) ampD 537639..538184 (+) 546 WP_000567319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OC536_RS02545 (CNRVC190247_00516) fldB 538335..538853 (-) 519 WP_000690112.1 flavodoxin FldB -
  OC536_RS02550 (CNRVC190247_00517) xerD 538991..539899 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  OC536_RS02555 (CNRVC190247_00518) dsbC 539916..540668 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62034.08 Da        Isoelectric Point: 6.0856

>NTDB_id=1152338 OC536_RS02525 WP_032476649.1 534096..535784(-) (pilB) [Vibrio cholerae strain CNRVC190247 isolate YE-NCPHL-18035-PI]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGVFHAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1152338 OC536_RS02525 WP_032476649.1 534096..535784(-) (pilB) [Vibrio cholerae strain CNRVC190247 isolate YE-NCPHL-18035-PI]
ATGCTGACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGCGTTTTTCACGCCCAAGAGCTGACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTACCGATTGCCAAGCAAGGCAATTTACTGCTGCTGGCGGTCTCTGACCCCACCTTATT
GCAAGCCGAAGAAGAGTTTCGCTTTGCCACTGGGCTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGTTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATTTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCGCAAGACGGGCGAATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGCGTTTCAACACTCCCAACCTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCACAAGGGATGATT
TTAATGACTGGCCCCACCGGCAGTGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTCAAAGCCGCGCAAACCGGTCACTTAGTGCTTTCCACTCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCCTCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTATATGAAGCCAATGCGGCGGGATGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCGAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGTTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.466

100

0.995
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

51.619

93.416

0.482
  pilB Acinetobacter baylyi ADP1

50.484

91.993

0.464
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37

100

0.395
  pilF Thermus thermophilus HB27

40.111

96.263

0.386
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB Haemophilus influenzae Rd KW20

39.279

93.772

0.368

Multiple sequence alignment