Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C6H39_RS23475 Genome accession   NZ_LT963409
Coordinates   4943484..4944701 (+) Length   405 a.a.
NCBI ID   WP_054995403.1    Uniprot ID   A0A0P9PNU3
Organism   Pseudomonas syringae isolate CFBP3840     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4938484..4949701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H39_RS23450 (CFBP3840_04548) - 4939061..4939612 (+) 552 WP_060403047.1 DUF1523 family protein -
  C6H39_RS23455 (CFBP3840_04549) - 4939660..4940247 (-) 588 WP_161938281.1 DUF433 domain-containing protein -
  C6H39_RS23460 - 4940516..4940839 (+) 324 Protein_4452 DUF1523 family protein -
  C6H39_RS23465 (CFBP3840_04550) - 4941135..4941563 (-) 429 WP_060404275.1 pilin -
  C6H39_RS23470 (CFBP3840_04551) pilB 4941787..4943481 (+) 1695 WP_060404276.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6H39_RS23475 (CFBP3840_04552) pilC 4943484..4944701 (+) 1218 WP_054995403.1 type II secretion system F family protein Machinery gene
  C6H39_RS23480 (CFBP3840_04553) pilD 4944703..4945575 (+) 873 WP_060404277.1 prepilin peptidase Machinery gene
  C6H39_RS23485 (CFBP3840_04554) coaE 4945572..4946195 (+) 624 WP_057422075.1 dephospho-CoA kinase -
  C6H39_RS23490 (CFBP3840_04555) yacG 4946192..4946401 (+) 210 WP_060404278.1 DNA gyrase inhibitor YacG -
  C6H39_RS23495 - 4946551..4946997 (-) 447 WP_099456531.1 hypothetical protein -
  C6H39_RS23500 (CFBP3840_04556) - 4947101..4948573 (-) 1473 WP_060404279.1 tetratricopeptide repeat protein -
  C6H39_RS23505 (CFBP3840_04557) - 4948570..4948956 (-) 387 WP_223282982.1 hypothetical protein -
  C6H39_RS23510 (CFBP3840_04558) - 4949000..4949629 (+) 630 WP_002553210.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44034.98 Da        Isoelectric Point: 10.1950

>NTDB_id=1148881 C6H39_RS23475 WP_054995403.1 4943484..4944701(+) (pilC) [Pseudomonas syringae isolate CFBP3840]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKAVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIGEGAENPNMRALVSSLKQEVSAGNSFAMALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVIGLSEVVQKWWLAIVGLFFASVFIFK
RAYKRSQKFRDSLDRFLLKIPVIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVVVGGLVIAMYLPIFK
LGDVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1148881 C6H39_RS23475 WP_054995403.1 4943484..4944701(+) (pilC) [Pseudomonas syringae isolate CFBP3840]
ATGGCCAGCAAGGCAGTCAAAGTCATAGTTTACACGTGGGAAGGTGTTGACAAGAAAGGCACCAAAACCAGTGGTGAACT
GAGCGGCCATAACCTCGCACTCGTAAAGGCCCAACTGCGCAAGCAAGGCATCAATCCGACCAAGGTGCGCAAGAAGGCTG
TATCGATCTTCGGCAAGGGCAAGAAGATCAAACCGCTGGATATCGCGTTCTTTTCTCGACAGATGGCAACGATGATGAAA
GCCGGAGTTCCCTTATTACAATCGTTCGACATCATCGGTGAGGGTGCAGAAAATCCAAATATGCGGGCGCTGGTGAGCTC
ACTCAAACAGGAGGTATCAGCAGGCAACAGCTTTGCAATGGCTTTACGGCAGAAGCCTGAGTATTTCGATGATCTTTTTT
GCAATCTGGTGGATGCCGGCGAGCAAGCAGGAGCTCTGGAGAGTTTGCTGGATCGAGTAGCGAGCTACAAAGAAAAAACA
GAGAAGCTCAAAGCAAAAATAAAGAAAGCAATGACCTACCCTGCCGCTGTGTTAATCGTGGCGGTCATCGTATCTGGCAT
TCTATTAATCAAAGTAGTCCCGCAGTTTCAGTCAGTATTCGCAGGATTCGGCGCCGAGCTACCTACATTCACTTTGATGG
TCATAGGACTTTCAGAAGTAGTTCAGAAGTGGTGGCTGGCGATTGTAGGCTTGTTTTTCGCTAGCGTCTTCATATTTAAG
CGCGCGTATAAGCGATCACAAAAATTTCGAGACAGCCTAGATCGTTTTTTACTTAAAATACCCGTCATTGGGCCGCTGAT
TTTCAAATCCTCAGTGGCGCGATATGCCCGTACTCTTGCAACTACTTTTGCGGCTGGGGTTCCTCTAGTAGAGGCTCTCG
ACTCTGTTGCAGGAGCAACCGGCAATGTAGTTTTCAGAAATGCAGTTAATAAAGTTAAGCAAGATGTATCCACAGGCATG
CAACTCAATTTCTCAATGCGATCAACCGGAGTTTTCCCGAGCTTGGCAATTCAGATGACTGCGATCGGAGAGGAGTCCGG
TGCTCTGGACAACATGCTGGACAAGGTCGCGACTTACTATGAGGATGAAGTCGACAATATGGTTGATAGCCTGACCAGCC
TAATGGAGCCCATGATCATGGCCGTCCTTGGGGTAGTAGTTGGCGGTCTAGTAATTGCGATGTACTTGCCAATATTCAAA
CTCGGGGATGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9PNU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

51.724

100

0.519
  pilG Neisseria gonorrhoeae MS11

43.073

98.025

0.422
  pilG Neisseria meningitidis 44/76-A

42.821

98.025

0.42
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

42.569

98.025

0.417
  pilC Thermus thermophilus HB27

39.401

99.012

0.39

Multiple sequence alignment