Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6H39_RS23470 Genome accession   NZ_LT963409
Coordinates   4941787..4943481 (+) Length   564 a.a.
NCBI ID   WP_060404276.1    Uniprot ID   A0AB35QV49
Organism   Pseudomonas syringae isolate CFBP3840     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4936787..4948481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H39_RS23445 (CFBP3840_04547) - 4937943..4938941 (-) 999 WP_054097660.1 ATP-grasp domain-containing protein -
  C6H39_RS23450 (CFBP3840_04548) - 4939061..4939612 (+) 552 WP_060403047.1 DUF1523 family protein -
  C6H39_RS23455 (CFBP3840_04549) - 4939660..4940247 (-) 588 WP_161938281.1 DUF433 domain-containing protein -
  C6H39_RS23460 - 4940516..4940839 (+) 324 Protein_4452 DUF1523 family protein -
  C6H39_RS23465 (CFBP3840_04550) - 4941135..4941563 (-) 429 WP_060404275.1 pilin -
  C6H39_RS23470 (CFBP3840_04551) pilB 4941787..4943481 (+) 1695 WP_060404276.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6H39_RS23475 (CFBP3840_04552) pilC 4943484..4944701 (+) 1218 WP_054995403.1 type II secretion system F family protein Machinery gene
  C6H39_RS23480 (CFBP3840_04553) pilD 4944703..4945575 (+) 873 WP_060404277.1 prepilin peptidase Machinery gene
  C6H39_RS23485 (CFBP3840_04554) coaE 4945572..4946195 (+) 624 WP_057422075.1 dephospho-CoA kinase -
  C6H39_RS23490 (CFBP3840_04555) yacG 4946192..4946401 (+) 210 WP_060404278.1 DNA gyrase inhibitor YacG -
  C6H39_RS23495 - 4946551..4946997 (-) 447 WP_099456531.1 hypothetical protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62452.71 Da        Isoelectric Point: 6.1627

>NTDB_id=1148880 C6H39_RS23470 WP_060404276.1 4941787..4943481(+) (pilB) [Pseudomonas syringae isolate CFBP3840]
MTDAVLTGLAKQLVLAELLTESVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDSDSALGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQVGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
IGTFKIYGPVGCEQCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1148880 C6H39_RS23470 WP_060404276.1 4941787..4943481(+) (pilB) [Pseudomonas syringae isolate CFBP3840]
ATGACTGATGCTGTCCTCACTGGCTTGGCCAAACAATTGGTCCTCGCCGAGCTACTTACTGAATCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAACTGGTCAAAAGTCTGACACTGG
CAGAGATGGCTTCCGACCAGTTTGGCGTGCCATTCATGGACCTCGCCAGTCTGGACAAAGAAAGCCAGCCTAAAGGCCTG
GTGAGTGAAAAGCTGGTTCGACAACATCACGCACTGCCGCTGTGGCGGCGCGGCAATAAACTATTTATTGGGATTTCGGA
CCCGACCAATCATCAGGCGGTGACTGATATTCAGTTCAGCACTGGCCTGAACACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACTATTGCTATAGATCGCTTTTTTGACAGTGATAGCGCTCTGGGTAATCTTGAAGATGTCGACCTGGGTCTGGAC
ATAGAACCGGCCGATGGCAAGGAAACCTCTCTAGCTACTCAAAATGACGCTGACGATGCTCCAGTAGTGCGATTCGTCAA
TAAAATGCTGATGGACGCTATCCGGCTTGGGTCATCCGACCTGCACTTTGAGCCCTACGAAAAGATCTTCCGCGTGCGGT
TGCGTACCGACGGTATCTTGCATGAAGTCGCCAGACCGCCCATACACTTGGCAAATCGTATTGCCGCGCGCCTGAAAGTA
ATGGCCAGTCTCGATATATCCGAGCGGCGCAAACCGCAGGACGGACGGGTCAAGTTAAGGGTGTCCAAAACCAAAGCCAT
CGATTTCCGTATGAATACCTTGCCTACGCTGTGGGGGGAGAAGATCGTGATGCGGATCCTCGATCCGACCAGTGCGCAGG
TGGGTATTGATGCTCTGGGCTACGAGCCAGAACAAAAAGCGCTGTACCTGGAGGCGCTGAAGCAGCCGCAAGGTATGATT
CTTGTAACCGGGCCCACGGGCTCGGGCAAAACGGTTTCCCTGTATACCGGCCTGAATATTCTCAACACTGTGGACATCAA
CATTTCCACGGCTGAAGACCCGGTAGAAATCAACCTGGAAGGCATTAATCAGGTCAACGTCAACCCGCGTCAGGGGCTGG
ACTTTTCTCAGGCTTTACGCGCATTCCTGCGTCAAGATCCAGACGTAATCATGGTCGGTGAGATACGAGACCTGGAAACC
GCCGAAATCGCCATCAAGGCATCGCAGACCGGCCATATGGTGCTATCCACACTGCACACCAACAGCGCTGCGGAAACGCT
GACACGACTGCATCATATGGGCGTTGCGGCGTTCAACATTGCTACAGCAATCAACCTGATTATTGCGCAGCGACTGGCAC
GTAAGCTGTGTAGCCACTGCAAGAAAGAACTGGATATTCCTCGCGAGACACTGATCCAGGAAGGCTTTCCGGAAGCAAAG
ATTGGCACTTTCAAAATCTATGGGCCAGTGGGATGTGAGCAGTGCAACGGGGGCTACAGGGGCAGGGTCGGTATTTACGA
AGTGGTCAAAAAAACACCTGAATTGGAGCGCATCATCATGGAAGAAGGCAACTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGATTTAATGACCTGCGAACCTCAGGGTTGTCCAAAGCCATGCAAGGCATCACCAGTCTTGAAGAAGTCAACCGC
GTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.582
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.575

100

0.557
  pilB Vibrio cholerae strain A1552

50.356

99.645

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.447

99.113

0.5
  pilB Vibrio campbellii strain DS40M4

49.293

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.128

99.468

0.479
  pilF Thermus thermophilus HB27

38.869

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.089

93.44

0.365

Multiple sequence alignment