Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C6H33_RS23010 Genome accession   NZ_LT962481
Coordinates   5098061..5099278 (+) Length   405 a.a.
NCBI ID   WP_024652511.1    Uniprot ID   A0A0N8SVH2
Organism   Pseudomonas syringae pv. syringae isolate CFBP2118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5093061..5104278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H33_RS22985 (CFBP2118_04376) - 5093341..5094033 (-) 693 WP_032636592.1 DUF433 domain-containing protein -
  C6H33_RS22990 (CFBP2118_04377) gstA 5094275..5094895 (-) 621 WP_024652515.1 glutathione transferase GstA -
  C6H33_RS22995 (CFBP2118_04378) - 5095050..5095442 (+) 393 WP_103378520.1 winged helix-turn-helix transcriptional regulator -
  C6H33_RS23000 - 5095703..5096137 (-) 435 WP_103378519.1 pilin -
  C6H33_RS23005 (CFBP2118_04379) pilB 5096364..5098058 (+) 1695 WP_024652512.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6H33_RS23010 (CFBP2118_04380) pilC 5098061..5099278 (+) 1218 WP_024652511.1 type II secretion system F family protein Machinery gene
  C6H33_RS23015 (CFBP2118_04381) pilD 5099280..5100152 (+) 873 WP_103378518.1 prepilin peptidase Machinery gene
  C6H33_RS23020 (CFBP2118_04382) coaE 5100149..5100772 (+) 624 WP_004415092.1 dephospho-CoA kinase -
  C6H33_RS23025 (CFBP2118_04383) yacG 5100769..5100978 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  C6H33_RS23030 (CFBP2118_04384) - 5101105..5101566 (-) 462 WP_024652509.1 hypothetical protein -
  C6H33_RS23035 (CFBP2118_04385) - 5101693..5102322 (+) 630 WP_003345130.1 DUF1780 domain-containing protein -
  C6H33_RS23040 (CFBP2118_04386) - 5102319..5102852 (+) 534 WP_003317783.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43992.98 Da        Isoelectric Point: 10.1848

>NTDB_id=1148575 C6H33_RS23010 WP_024652511.1 5098061..5099278(+) (pilC) [Pseudomonas syringae pv. syringae isolate CFBP2118]
MASKAVKVIVYTWEGVDKKGTKASGELSGHNMALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVSSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAIIVSGILLIKVVPQFQSVFAGFGADLPAFTLMVIGLSNIVQEWWLIIVGLLFAGFFLFK
RAYKKSQKFRDGLDRLLLKAPLIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFK
LGSIV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1148575 C6H33_RS23010 WP_024652511.1 5098061..5099278(+) (pilC) [Pseudomonas syringae pv. syringae isolate CFBP2118]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACGTGGGAAGGTGTGGACAAGAAGGGCACTAAAGCCAGTGGCGAACT
GAGCGGCCATAATATGGCACTCGTAAAAGCCCAATTGCGCAAGCAAGGCATCAACCCCACCAAGGTGCGCAAGAAGTCCG
CATCGATCTTCGGCAAAGGCAAGAAGATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTGCCGTTGCTGCAGTCGTTTGACATCATCAGCGAGGGCGCTGAAAACCCAAACATGCGTACACTTGTGAGCTC
GTTAAAGCAGGAAGTGTCGGCAGGCAACAGCTTTGCGACGGCGTTGCGACAGAAACCGGAATATTTCGATGACCTGTTTT
GCAATCTGGTAGATGCCGGCGAGCAAGCGGGGGCACTTGAAAGTCTTCTGGACCGGGTCGCTAGCTACAAGGAGAAGACC
GAAAAACTCAAAGCAAAAATCAAAAAAGCCATGACGTACCCAATCGCCGTGTTGATCGTAGCGATTATTGTGTCAGGTAT
TTTGTTGATCAAGGTGGTACCGCAGTTCCAATCCGTATTTGCTGGCTTTGGTGCTGACTTACCTGCGTTTACTCTGATGG
TTATTGGTCTATCAAACATTGTTCAGGAATGGTGGCTGATAATTGTCGGTTTATTGTTTGCAGGTTTTTTTTTATTTAAG
CGGGCCTACAAAAAATCTCAAAAATTTCGCGATGGCCTTGATCGTCTTTTGCTAAAAGCCCCCCTCATCGGGCCTCTTAT
TTTCAAATCTTCGGTAGCTCGCTATGCACGCACACTTGCTACGACTTTTGCCGCTGGTGTACCGCTTGTAGAGGCGTTGG
ACTCGGTTGCTGGAGCAACGGGCAATGTAGTGTTCAAAAATGCAGTCAACAAGGTCAAGCAAGACGTTTCCACAGGTATG
CAATTGAATTTCTCGATGCGATCTACGGGAGTATTCCCAAGCCTTGCGATCCAGATGACAGCCATTGGCGAAGAATCCGG
CGCCTTGGACACCATGCTCGACAAGGTAGCAACCTATTACGAGGATGAAGTCGATAACATGGTCGACAACCTGACCAGCC
TGATGGAACCGATGATCATGGCCTTTTTGGGAGTGATCGTCGGCGGCCTTGTCATTGCCATGTACCTCCCCATCTTCAAA
CTTGGAAGCATTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0N8SVH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Legionella pneumophila strain ERS1305867

52.217

100

0.523
  pilC Vibrio campbellii strain DS40M4

42.75

98.765

0.422
  pilG Neisseria gonorrhoeae MS11

42.714

98.272

0.42
  pilG Neisseria meningitidis 44/76-A

42.714

98.272

0.42
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment