Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6H33_RS23005 Genome accession   NZ_LT962481
Coordinates   5096364..5098058 (+) Length   564 a.a.
NCBI ID   WP_024652512.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae isolate CFBP2118     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5091364..5103058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H33_RS22975 (CFBP2118_04374) - 5092347..5092892 (+) 546 WP_003317774.1 DUF1523 family protein -
  C6H33_RS22980 (CFBP2118_04375) - 5092937..5093344 (-) 408 WP_024652517.1 hypothetical protein -
  C6H33_RS22985 (CFBP2118_04376) - 5093341..5094033 (-) 693 WP_032636592.1 DUF433 domain-containing protein -
  C6H33_RS22990 (CFBP2118_04377) gstA 5094275..5094895 (-) 621 WP_024652515.1 glutathione transferase GstA -
  C6H33_RS22995 (CFBP2118_04378) - 5095050..5095442 (+) 393 WP_103378520.1 winged helix-turn-helix transcriptional regulator -
  C6H33_RS23000 - 5095703..5096137 (-) 435 WP_103378519.1 pilin -
  C6H33_RS23005 (CFBP2118_04379) pilB 5096364..5098058 (+) 1695 WP_024652512.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6H33_RS23010 (CFBP2118_04380) pilC 5098061..5099278 (+) 1218 WP_024652511.1 type II secretion system F family protein Machinery gene
  C6H33_RS23015 (CFBP2118_04381) pilD 5099280..5100152 (+) 873 WP_103378518.1 prepilin peptidase Machinery gene
  C6H33_RS23020 (CFBP2118_04382) coaE 5100149..5100772 (+) 624 WP_004415092.1 dephospho-CoA kinase -
  C6H33_RS23025 (CFBP2118_04383) yacG 5100769..5100978 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  C6H33_RS23030 (CFBP2118_04384) - 5101105..5101566 (-) 462 WP_024652509.1 hypothetical protein -
  C6H33_RS23035 (CFBP2118_04385) - 5101693..5102322 (+) 630 WP_003345130.1 DUF1780 domain-containing protein -
  C6H33_RS23040 (CFBP2118_04386) - 5102319..5102852 (+) 534 WP_003317783.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62457.84 Da        Isoelectric Point: 6.4532

>NTDB_id=1148574 C6H33_RS23005 WP_024652512.1 5096364..5098058(+) (pilB) [Pseudomonas syringae pv. syringae isolate CFBP2118]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASLDKESLPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDTGLGNLEDVDLGLD
IEPADGKETSLAAQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIKEGFPEAK
IGTFKIYGPMGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1148574 C6H33_RS23005 WP_024652512.1 5096364..5098058(+) (pilB) [Pseudomonas syringae pv. syringae isolate CFBP2118]
ATGACTGATGCTGTCCTCACTGGCCTGGCCAAACAATTGGTCCTAGCCGAGCTACTTACTGAACCTGTTGCGCAGCAAGC
GTATCAGCAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGCACAAACTGGTCAAAAGCCTGACGCTGG
CAGAATTGGCCTCCGATCAATTCGGCGTGCCGTTCATGGACCTCGCCAGCTTGGACAAGGAAAGCCTGCCTAAAGGCCTG
GTGAGTGAAAAGCTGGTTCGACAGCATCACGCCCTGCCGCTGTGGCGACGCGGTAATAAGCTATTTATCGGGATTTCCGA
CCCTACGAATCATCAGGCAGTCACTGATATTCAGTTCAGCACTGGCCTGAACACCGAAGCGATTCTGGTAGAAGATGACA
AGCTGACCACTGCAATAGATCGCTTTTTTGACAGTGATACCGGTCTAGGCAATCTGGAAGATGTCGATCTGGGCCTGGAC
ATCGAGCCCGCGGATGGCAAGGAGACCTCGCTCGCTGCGCAGAACGACGCGGATGACGCTCCAGTGGTACGTTTCGTCAA
CAAAATGCTTATGGATGCCATCCGATTAGGATCATCCGACTTGCACTTTGAGCCCTACGAAAAGATCTTCCGCGTACGTT
TGCGTACCGATGGCATCCTGCACGAAGTCGCCAGACCGCCGATTCATTTGGCAAACCGTATTGCTGCGCGTCTGAAGGTC
ATGGCCAGCCTCGATATTTCGGAACGACGCAAACCGCAGGACGGAAGGGTAAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAATACCCTGCCCACATTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGCGCACAGA
TGGGCATCGATGCTCTGGGCTACGAGCCAGAACAAAAGGCGTTGTATCTGGAAGCGTTGAAGCAGCCGCAAGGTATGATT
CTTGTGACCGGCCCGACAGGCTCCGGCAAGACAGTGTCGCTGTATACCGGCCTGAATATTCTTAATACCGTGGACATCAA
CATATCCACAGCCGAAGACCCGGTAGAGATCAACCTGGAAGGTATCAATCAGGTCAACGTCAACCCGCGCCAAGGGCTGG
ACTTTTCTCAGGCATTGCGCGCGTTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATTCGCGACCTGGAAACC
GCCGAAATCGCTATTAAGGCGTCGCAAACCGGCCACATGGTGCTTTCAACGCTGCACACCAACAGCGCAGCAGAAACATT
GACTCGACTGCATCATATGGGCGTTGCAGCGTTCAACATTGCCACCGCAATAAACCTGATTATTGCGCAGCGACTGGCGC
GCAAGCTGTGTAGCCACTGCAAGAAAGAGCTCGATATTCCTCGCGAGACATTGATTAAAGAAGGCTTTCCAGAGGCGAAG
ATCGGTACGTTCAAGATCTATGGGCCGATGGGCTGCGAGCATTGCAATGGTGGTTATAGAGGCCGAGTCGGCATTTATGA
GGTGGTCAAGAAAACAGCGGAGCTGGAGCGCATTATCATGGAAGAAGGTAACTCACTTGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATCTGCGAACCTCGGGCCTGTCAAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.244

100

0.574
  pilB Acinetobacter baylyi ADP1

56.36

100

0.566
  pilB Legionella pneumophila strain ERS1305867

55.398

100

0.555
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.447

99.113

0.5
  pilB Vibrio campbellii strain DS40M4

49.293

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.221

99.645

0.481
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

93.262

0.362

Multiple sequence alignment