Detailed information NTG1148576

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C6H33_RS23015
• Genome accession: NZ_LT962481
• Coordinates: 5099280..5100152 (+)
• Length: 290 a.a.
• NCBI ID: WP_103378518.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae isolate CFBP2118
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5094280..5105152

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H33_RS22995 (CFBP2118_04378)	-	5095050..5095442 (+)	393	WP_103378520.1	winged helix-turn-helix transcriptional regulator	-
C6H33_RS23000	-	5095703..5096137 (-)	435	WP_103378519.1	pilin	-
C6H33_RS23005 (CFBP2118_04379)	pilB	5096364..5098058 (+)	1695	WP_024652512.1	type IV-A pilus assembly ATPase PilB	Machinery gene
C6H33_RS23010 (CFBP2118_04380)	pilC	5098061..5099278 (+)	1218	WP_024652511.1	type II secretion system F family protein	Machinery gene
C6H33_RS23015 (CFBP2118_04381)	pilD	5099280..5100152 (+)	873	WP_103378518.1	prepilin peptidase	Machinery gene
C6H33_RS23020 (CFBP2118_04382)	coaE	5100149..5100772 (+)	624	WP_004415092.1	dephospho-CoA kinase	-
C6H33_RS23025 (CFBP2118_04383)	yacG	5100769..5100978 (+)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
C6H33_RS23030 (CFBP2118_04384)	-	5101105..5101566 (-)	462	WP_024652509.1	hypothetical protein	-
C6H33_RS23035 (CFBP2118_04385)	-	5101693..5102322 (+)	630	WP_003345130.1	DUF1780 domain-containing protein	-
C6H33_RS23040 (CFBP2118_04386)	-	5102319..5102852 (+)	534	WP_003317783.1	MOSC domain-containing protein	-
C6H33_RS23050 (CFBP2118_04387)	-	5103069..5104367 (+)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32004.17 Da
• Isoelectric Point: 8.1558

>NTDB_id=1148576 C6H33_RS23015 WP_103378518.1 5099280..5100152(+) (pilD) [Pseudomonas syringae pv. syringae isolate CFBP2118]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIH
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
ALLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1148576 C6H33_RS23015 WP_103378518.1 5099280..5100152(+) (pilD) [Pseudomonas syringae pv. syringae isolate CFBP2118]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTTTACCGCCTCCCGATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAATCTGAATCGCCCCCGTTCAAGCTGCCCGCATTGCGCACACAAAATCCAC
CCATGGGAAAACCTGCCGGTGATCAGTTATCTGCTGCTGCGCGGTAAATGTTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGTTGGTGGAGCTGACCTGCGCAGTGCTATCAGCCTACGTAGCCTGGCATTTCGGTTTCGGCTGGCAGGCGGCGG
CGATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGTTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTCACGTCGCTGAATGATGCGCTATGGGGTGCAGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGCTGCTCGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.004	97.586	0.517
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.19	94.483	0.493
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Neisseria gonorrhoeae MS11	50.746	92.414	0.469