Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PL963_RS04230 Genome accession   NZ_LT962480
Coordinates   906171..907388 (-) Length   405 a.a.
NCBI ID   WP_096104049.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae isolate CFBP4215     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 901171..912388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL963_RS04200 (CFBP4215_00793) - 902597..903130 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  PL963_RS04205 (CFBP4215_00794) - 903127..903756 (-) 630 WP_060400845.1 DUF1780 domain-containing protein -
  PL963_RS04210 (CFBP4215_00795) - 903883..904344 (+) 462 WP_024643094.1 hypothetical protein -
  PL963_RS04215 (CFBP4215_00796) yacG 904471..904680 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PL963_RS04220 (CFBP4215_00797) coaE 904677..905300 (-) 624 WP_004415092.1 dephospho-CoA kinase -
  PL963_RS04225 (CFBP4215_00798) pilD 905297..906169 (-) 873 WP_103714216.1 prepilin peptidase Machinery gene
  PL963_RS04230 (CFBP4215_00799) pilC 906171..907388 (-) 1218 WP_096104049.1 type II secretion system F family protein Machinery gene
  PL963_RS04235 (CFBP4215_00800) pilB 907391..909085 (-) 1695 WP_104683674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PL963_RS04240 - 909313..909747 (+) 435 WP_096104047.1 pilin -
  PL963_RS04245 (CFBP4215_00801) - 909971..910516 (-) 546 WP_010415546.1 DUF1523 family protein -
  PL963_RS04250 (CFBP4215_00802) - 910636..911634 (+) 999 WP_104683676.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44194.11 Da        Isoelectric Point: 10.1809

>NTDB_id=1148535 PL963_RS04230 WP_096104049.1 906171..907388(-) (pilC) [Pseudomonas syringae pv. syringae isolate CFBP4215]
MASKAVKVSIYTWEGLDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSISIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVVSLKQEVSAGNSFATALRQKPEHFDELFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPTAVLVVAVIVSGILLIKVVPQFQTVFSSFGADLPLFTLMVIALSEFVQQWWLAILGLLFGSFFLFK
RAYKQSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVHKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1148535 PL963_RS04230 WP_096104049.1 906171..907388(-) (pilC) [Pseudomonas syringae pv. syringae isolate CFBP4215]
ATGGCCAGCAAAGCAGTAAAAGTCAGCATTTATACTTGGGAAGGCCTGGACAAAAAGGGTGGAAAGCTCAGTGGAGAATT
GAGCGGCCACAACCCAGCGCTGATAAAAGCGCAGTTGCGCAAGCAGGGCGTCAACCCGACCAAAGTACGTAAGAAGTCAA
TTTCTATATTTGGAAAAGGTAAAAAAATCAAGCCACTGGACATAGCGTTCTTCTCCAGGCAGATGGCCACCATGATGAAA
GCCGGCGTACCTCTACTCCAATCGTTCGACATAATCAGCGAGGGTGCCGAGAACCCCAATATGCGAACGCTCGTGGTATC
GCTAAAGCAAGAAGTCTCAGCAGGGAACAGCTTCGCGACTGCCTTGCGCCAAAAGCCTGAACATTTTGATGAGCTGTTCT
GCAACCTCGTTGATGCAGGTGAACAGGCAGGCGCATTGGAAAGTCTTCTGGACAGGGTAGCAACGTATAAGGAAAAAACC
GAAAAGCTCAAGGCCAAGATCAAAAAAGCCATGACCTATCCTACTGCTGTTCTCGTGGTGGCGGTAATAGTTTCAGGTAT
TCTGCTAATCAAAGTGGTACCGCAATTTCAGACAGTATTTTCAAGTTTTGGTGCAGATTTACCTCTGTTTACACTGATGG
TGATTGCCCTTTCAGAGTTTGTGCAGCAGTGGTGGCTAGCTATTCTGGGACTGCTATTTGGAAGCTTTTTTCTGTTCAAA
CGGGCCTACAAACAATCTCAGAAATTTCGCGACAGCCTAGACAGGTTCTTGTTAAAAGTCCCAATCATAGGCCCGCTTAT
CTTTAAATCATCAGTAGCCCGCTATGCGCGCACTCTTGCCACGACTTTCGCTGCTGGCGTACCGCTGGTCGAGGCGCTGG
ACTCGGTGGCCGGGGCGACAGGGAATGTGGTGTTTCGAAATGCAGTTCATAAAGTAAAGCAGGATGTCTCCACCGGCATG
CAATTGAATTTCTCGATGCGCTCCACAGGTGTATTTCCAAGTTTAGCGATCCAGATGACAGCCATCGGTGAGGAATCTGG
CGCGCTCGACTCCATGCTCGATAAAGTTGCAACCTACTACGAAGATGAAGTCGATAACATGGTTGATAGCCTAACCAGCC
TGATGGAACCCATGATCATGGCTGTGCTGGGTGTCATCGTTGGCGGCCTTGTCATTGCGATGTACCTCCCCATTTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilC Vibrio campbellii strain DS40M4

42.015

100

0.422
  pilG Neisseria gonorrhoeae MS11

42.184

99.506

0.42
  pilG Neisseria meningitidis 44/76-A

41.935

99.506

0.417
  pilC Vibrio cholerae strain A1552

40.494

100

0.405
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment